[1]薛永国,刘鑫磊,曹旦,等.大豆体细胞胚诱导再生的影响因素和相关基因的研究进展[J].大豆科学,2018,37(03):464-470.[doi:10.11861/j.issn.1000-9841.2018.03.0464]
　XUE Yong-guo,LIU Xin-lei,CAO Dan,et al.Research Progress on Related Genes and Factors Influencing Somatic Embryo Induction and Regeneration from Immature Cotyledon of Soybean[J].Soybean Science,2018,37(03):464-470.[doi:10.11861/j.issn.1000-9841.2018.03.0464]
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大豆体细胞胚诱导再生的影响因素和相关基因的研究进展

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年03期 页码: 464-470 栏目: 出版日期: 2018-05-20
Title:
Research Progress on Related Genes and Factors Influencing Somatic Embryo Induction and Regeneration from Immature Cotyledon of Soybean
作者:
薛永国; 刘鑫磊; 曹旦; 栾晓燕
(黑龙江省农业科学院 大豆研究所,黑龙江 哈尔滨 150086)
Author(s):
XUE Yong-guo;  LIU Xin-lei;  CAO Dan;  LUAN Xiao-yan
(Soybean Research Institute,Heilongjiang Academy Agricultural of Sciences, Harbin 150086，China)
关键词:
大豆;  体细胞胚;  影响因素;  再生基因
Keywords:
Soybean;  Somatic embryo;  Influencing factors;  Regeneration gene
DOI:
10.11861/j.issn.1000-9841.2018.03.0464
文献标志码:
A
摘要:
大豆是世界上重要的油料和经济作物,也是种植面积最大的转基因作物。大豆体细胞胚再生途径的转基因方法至今仍没有较大突破,主要原因是体细胞胚诱导再生频率低且不稳定，同时受基因型影响明显，这些原因限制了其在不同大豆品种中的应用。文章对影响大豆体细胞胚频率的基因型、激素、pH及其它因素进行了梳理总结，从同源克隆、基因定位和重测序等方面对体细胞胚诱导再生的相关基因的研究进展情况进行了详细综述。并对大豆体细胞胚诱导再生的应用前景和存在问题进行了归纳分析，旨在为大豆体细胞胚诱导再生的进一步研究提供参考。
Abstract:
Soybean is the most important oil and cash crops and the transgenic crops with the largest planting area in the world. There is still no major breakthrough in the transgenic method of soybean somatic embryo regeneration. The low and unstable induction and regeneration frequency of somatic embryos is the main reason, and it is obviously influenced by genotypes. Those reasons limit its application in different soybean cultivars. This paper summarized the factors affecting the regeneration of somatic embryos of soybean. The genotypes, hormones, pH and other factors that influence the frequency of somatic embryogenesis in soybean were summarized. The research progress of somatic embryo induction and regeneration related genes was reviewed in detail from homologous cloning, gene mapping and resequencing. The application prospect and existing problems of somatic embryogenesis and regeneration in soybean were also summarized and analyzed aiming purpose is to provide directions and reference for further research on somatic embryogenesis and regeneration in soybean.

参考文献/References:

［1］王鹏飞, 刘丽君, 唐晓飞, 等. 适于体细胞胚发生的大豆基因型筛选 ［J］. 大豆科学,2013, 32(1): 43-45.(Wang P F, Liu L J, Tang X F, et al. Screening of soybean genotypes suitable for somatic embryogenesis［J］.Soybean Science,2013, 32 (1): 43-45.)
［2］Cheng T Y, Saka H, Voqui-Dinh T H. Plant regeneration from soybean cotyledonary node segments in culture［J］. Plant Science Letters, 1980, 19(2): 91-99.?
［3］Kartha K K, Pahl K, Leung N L, et al. Plant regeneration from meristems of grain legumes: Soybean, cow bean, peanut, chickpea,and bean［J］. Canadian Journal of Botany, 1981, 59: 1671-1679.
［4］Barw ale U B, Kerns H R, Widholm J M. Pant regeneration from callus cultures of several soybean genotypes via embryogenesis and organ genesis［J］. Planta, 1986,167: 473-481.
［5］Wright M S, Ward D V, Hinchee M A, et al. Regeneration of soybean (Glycine max L. Merr.) from cultured primary leaf tissue ［J］. Plant Cell Reports, 1987, 6: 83-89.
［6］Kim J, LaMotte C E, Hack E. Plant regeneration in vitro from primary leaf nodes of soybean (Glycine max) seedlings［J］. Plant Physiology, 1990, 136: 664-669.
［7］Finer J J, Nagasawa A. Development of an embryogenic suspension culture of soybean (Glycine max Merrill.)［J］. Plant Cell, Tissue and Organ Culture, 1988, 15(2): 125-136.
［8］侯文胜,林抗雪,陈普,等. 大豆规模化转基因技术体系的构建及其应用［J］. 中国农业科学,2014,47(21): 4198-4210.(Hou W S, Lin K X, Chen P, et al. Establishment and prospect of efficient transformation systems for soybean［J］. Scientia Agricultura Sinica, 2014,47(21): 4198-4210.)
［9］程琳静, 闫军辉, 钟云鹏, 等. 大豆高效体细胞胚诱导和增殖方法的研究［J］. 大豆科学, 2014,33(3): 305-310.(Cheng L J, Yan J H, Zhong Y P, et al. Research to the effective methods of soybean somatic embryo induction and proliferation［J］. Soybean Science, 2014,33(3): 305-310.)
［10］Lazzeri P A, Hildebrand D F, Collins G B. A procedure for plant regeneration from immature cotyledon tissue of soybean［J］. Plant Molecular Biology Reporter, 1985, 3(4): 160-168.?
［11］Ranch J P, Oglesby L, Zielinski A C, et al. Plant regeneration from embryo-derived tissue cultures of soybeans［J］. In Vitro Cellular & Developmental Biology, 1985, 21(11): 653-658.
［12］Finer J J, McMullen M D. Transformation of soybean via particle bombardment of embryogenic suspension culture tissue［J］. In Vitro Cellular & Developmental Biology-Plant, 1991, 27(4): 175-182.?
［13］Christianson M L, Warnick D A, Carlson P S. A morphogenetically competent soybean suspension culture［J］. Science, 1983, 222(4624): 632-635.
［14］刘艳芝,赵桂兰,刘莉,等. 大豆幼胚子叶诱导胚胎发生［J］. 吉林农业科学,1999(6):16-18.（Liu Y Z, Zhao G L, Liu L, et al. The induction of embryogenesis from immature cotyledon of soybean［J］. Journal of Jilin Agricultural Sciences, 1999(6):16-18.）
［15］张淑珍,徐鹏飞,林世锋,等. 大豆体细胞胚再生体系的研究进展及展望［J］. 大豆科学,2004,23(3):232-235.(Zhang S Z, Xu P F, Lin S F, et al. Recent advances and prospect on soybean somatic embryogenesis system［J］.Soybean Science, 2004,23(3):232-235.)
?［16］王萍,王罡,季静,等. 大豆体细胞胚胎发生与农杆菌介导的遗传转化［J］. 遗传,2004(5):695-700.(Wang P, Wang G, Ji J, et al. Studies of somatic embryogenesis and genetic transformation by Agrobacterium-mediated in soybean［J］. Hereditas, 2004(5):695-700.)
［17］李宁辉,徐鹏飞,范素杰,等.大豆品种合丰25体细胞胚发生条件的优化［J］.作物杂志,2011(4):23-27. (Li N H, Xu P F, Fan S J, et al. Optimizing of regeneration conditions of somatic embryo for soybean variety ‘Hefeng 25’［J］.Crops, 2011(4):23-27.)
［18］李海燕,朱延明,刘北东,等. 大豆幼胚子叶体细胞胚诱导主要影响因素的研究［J］. 作物学报,2002,28(6):852-856.(Li H Y, Zhu Y M, Liu B D, et al. Study on main factors influencing somatic embryo inducting from immature cotyledon of soybean (Glycine max L.) ［J］.Acta Agronomic Sinica, 2002,28(6):852-856.)
［19］van K, Hyun J J, Jang Y E, et al. Regeneration of plants from EMS-treated immature embryo cultures in soybean ［Glycine max (L.) Merr.］ ［J］. Journal of Crop Science Biotechnology, 2008, 11 (2): 119-126.
［20］周思君,尹光初,雷勃钧,等,从大豆幼胚诱导胚胎发生再生植株［J］.大豆科学,1989,8（1）:39-44.（Zhou S J, Yin G C, Lei B J, et al. From soybean young embryo induced embryogenesis regeneration plant［J］.Soybean Science, 1989,8（1）:39-44.）
［21］王萍,吴颖,杨武杰,等.大豆未成熟子叶体细胞胚胎发生及其相关因子的分析［J］.中国油料作物学报,2002,24(1):29-32.(Wang P, Wu Y, Yang W J, et al. Somatic embryogenesis from immature cotyledons of soybean and analysis of correlative factors［J］.Chinese Journal of Oil Crop Sciences, 2002,24(1):29-32.)
［22］曲桂芹,张贤泽,霍俊伟.大豆体细胞胚的成熟处理及植株再生［J］.东北农业大学学报, 2002 , 33 (1) : 82-89 .(Qu G Q, Zhang X Z, Huo J W. Maturation of soybean somatic embryos and the plant regeneration［J］.Journal of Northeast Agricultural University, 2002, 33(1): 82-89.)
［23］王晓春,刘尚前,季静,等.影响大豆体细胞胚萌发率的因素［J］.大豆科学, 2004, 23(2): 151-154.(Wang X C, Liu S Q, Ji J, et al. The affect factors on the germination frequency of somatic embryos of soybean ［J］.Soybean Science, 2004, 23(2): 151-154.)
［24］Chugh A, Khurana P. Gene expression during somatic embryogenesis［J］. Current Science, 2002, 83(6):715-723.
［25］Lotan T, Ohto M, Matsudaira Y K, et al. Arabidopsis LEAFYCOTYLEDON1 is sufficient to induce embryo development invegetative cells［J］. Cell, 1998, 93(6): 1195-1205.
［26］Zhang S, Wong L, Meng L, et al. Similarity of expression patterns of knotted1 and Zm LEC1 during somatic and zygotic embryo-genesis in maize (Zea mays L)［J］. Planta, 2002, 215(2):191-194.
［27］Yazawa K, Takahata K, Kamada H. Isolation of the gene encoding carrot leafy cotyledon1 and expression analysis during somatic and zygotic embryogenesis［J］. Plant Physiology and Biochemistry, 2004, 42(3): 215-223.
［28］Fambrini M, Durante C, Cionini G, et al. Characterization of LEAFY COTYLEDON1-LIKE gene in Helianthus annuus?and its relationship with zygotic and somatic embryogenesis［J］. Development Genes and Evolution, 2006, 216(5): 253-264.
［29］李爱芹,夏晗,王兴军, 等.花生LEC1基因的克隆及表达研究［J］.西北植物学报, 2009, 29(9): 1730-1735.(Li A Q,Xia H, Wang X J, et al. Cloning and expression analysis of peanut (Arachis hypogaea L.)［J］.Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(9): 1730-1735.
［30］Stone S L, Kwong L W, Yee K M, et al. LEAFY COTYLEDON2 encodes a B3 domain transcription factor that induces embryo development［J］. Proceedings of the National Academy of Sciences, 2001, 98(20): 11806-11811.?
［31］武小霞,王敏,陈庆山,等.大豆再生相关基因Gm LEC的克隆及生物信息学分析［J］.东北农业大学学报,2015,46(4): 1-9.(Wu X X, Wang M, Chen Q S, et al. Cloning and bioinformatics analysis of regeneration related gene GmLEC?in soybean［J］. Journal of Northeast Agricultural University, 2015,46(4): 1-9.)
［32］杨超. 大豆植株再生和遗传转化技术体系的研究［D］.南京:南京农业大学,2009.(Yang C. Study on technological system of plant regeneration and genetic transformation in soybean ［Glycine max (L.) Merr.］ ［D］.Nanjing:Nanjing Agricultural University,2009.)
［33］Thakare D, Tang W, Hill K, et al. The MADS-domain transcriptional regulator AGAMOUS-LIKE15 promotes somatic embryo development in Arabidopsis and soybean［J］. Plant Physiology, 2008, 146(4): 1663-1672.
［34］Zheng Q, Zheng Y, Ji H, et al. Gene regulation by the AGL15 transcription factor reveals hormone interactions in somatic embryogenesis［J］. Plant Physiology, 2016, 172(4): 2374-2387.
［35］Perry S E, Zheng Q, Zheng Y. Transcriptome analysis indicates that GmAGAMOUS-Like 15 may enhance somatic embryogenesis by promoting a dedifferentiated state［J］. Plant Signaling & Behavior, 2016, 11(7): e1197463.
［36］Li S N, Su A Y, Yu Y C, et al.Bioinformatics and expression analysis of regeneration related gene GmARF in soybean［J］. Genomics and Applied Biology, 2015, 34(10): 2187-2193.
［37］Zhang C, Wu X, Zhang B, et al. Functional analysis of the GmESR1?gene associated with soybean regeneration［J］. PloS One, 2017, 12(4): e0175656.
［38］Rose R J, Sheahan M B, Tiew T W Y. Connecting stress to development in the induction of somatic embryogenesis［M］// Aslam J, Srivastava P S, Sharma M P. Somatic embryogenesis and gene expression. New Delhi: Narosa Publishing House, 2013: 146-165.
39］Xing S, Quodt V, Chandler J, et al. SPL8 acts together with the brassinosteroid-signaling component BIM1 in controlling Arabidopsis thaliana?male fertility［J］. Plants, 2013, 2(3): 416-428.

［40］Sun J, Li J, Liu M, et al. Construction and analysis of a suppression subtractive hybridization library of regeneration-related genes in soybean［J］. Genetics and Molecular Research: GMR, 2015, 14(1): 763-773.
［41］Thibaud-Nissen F, Shealy R T, Khanna A, et al. Clustering of microarray data reveals transcript patterns associated with somatic embryogenesis in soybean［J］. Plant Physiology, 2003, 132(1): 118-136.
［42］Song X, Han Y, Teng W, et al. Identification of QTL underlying somatic embryogenesis capacity of immature embryos in soybean (Glycine max (L.) Merr.)［J］. Plant cell reports, 2010, 29(2): 125-131.
［43］Mookkan M, Nelson-Vasilchik K, Hague J, et al. Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2［J］. Plant Cell Reports, 2017, 36(9): 1477-1491.
［44］Horstman A, Li M, Heidmann I, et al. The BABY BOOM transcription factor activates the LEC1-ABI3-FUS3-LEC2 network to induce somatic embryogenesis［J］. Plant Physiology, 2017, 175(2): 848.
［45］Ge F, Huang X, Hu H, et al. Endogenous small interfering RNAs associated with maize embryonic callus formation［J］. PloS One, 2017, 12(7): e0180567.
［46］Liu Y, Zhang Z, Fu J, et al. Transcriptome analysis of maize immature embryos reveals the roles of cysteine in improving Agrobacterium infection efficiency［J］. Frontiers in Plant Science, 2017, 8: 1778.
［47］Stewart C N J, Adang M J, All J N, et al．Genetic transformation recovery and characterization of fertile soybean transgenic for a synthetic Bacillus thuringiens is cry1Ac?gene［J］．Plant Physiology,1996,112(1): 121-129．
［48］Santarem E R, Pelissier B, Finer J J. Effect of explant orientation, pH, solidifying agent andwounding on initiation of soybean somatic embryos［J］. In Vitro Cellular & Developmental Biology-Plant, 1997, 33(1): 13-19.
［49］Hadi M Z,McMullen M D,Finer J J．Transformation of 12 different plasmids into soybean via particle bombardment ［J］．Plant Cell Reports,1996,15(7) : 500-505．
［50］Maughan P J, Philip R, ChoM J, et al. Biolistic transformation, expression, and inheritance of bovine beta-casein in soybean (Glycine max) ［J］． In Vitro Cellular ＆ Developmental Biology-Plant, 1999,35(4) : 344-349．
［51］Aragao F J L, Sarokin L, Vianna G R, et al． Selection of transgenic meristematic cells utilizing a herbicidal molecule results in the recovery of fertile transgenic soybean［Glycine max(L.) Merrill］ plants at a high frequency［J］．Theoretical and Applied Genetics,2000,101: 1-6．
［52］Walker D R, Parrott W A. Effect of polyethylene glycol and sugar alcohols on soybean somatic embryo germination and conversion［J］. Plant Cell, Tissue and Organ Culture, 2001, 64(1): 55-62.
［53］Santarem E R, Pelissier B, Finer J J. Effect of explant orientation, pH, solidifying agent and wounding on initiation of soybean somatic embryos［J］. In Vitro Cellular & Developmental Biology-Plant, 1997, 33(1): 13-19.
［54］邱波，王志坤，孟凡立，等. 不同大豆基因型再生性及对农杆菌敏感性的研究［J］. 大豆科学，2011,30（5）：752-756.（Qiu B, Wang Z K, Meng F L, et al. Regeneration and sensitivity to Agrobacterium of different soybean genotypes ［J］. Soybean Science,2011,30（5）：752-756.）
［55］Sung Z R. Mutagenesis of cultured plant cells ［J］. Genetics, 1976, 84: 51-57.
［56］Tfuii J.The mutagenic effect of EMS on somatic mutation (Ml) and recessive mutation (M2) of soybean［J］. Environmental and Experimental Botany,1986,26(2): 191-195.
［57］Chen Y L, Liang H L, Ma X L, et al. An efficient rice mutagenesis system based on suspension-cultured cells ［J］. Journal of Integrative Plant Biology, 2013, 55(2): 122-130.
［58］Abe A, Kosugi S, Yoshida K, et al. Genome sequencing reveals agronomically important loci in rice using MutMap ［J］. Nature Biotechnology, 2012, 30(2):174-178.
［59］Hofmann N E, Raja R, Nelson R L, et al. Mutagenesis of embryogenic cultures of soybean and detecting polymorphisms using RAPD markers ［J］. Biologia Plantarum, 2004, 48(2): 173-177.
［60］Hiroki T, Muluneh T, Akira A, et al. MutMap accelerates breeding of a salt-tolerant rice cultivar ［J］. Nature Biotechnology, 2015, 33(5): 445-449.
［61］曲颖, 李文建, 周利斌, 等. 重离子辐射植物的诱变效应研究及应用［J］. 原子核物理评论, 2007, 24(4): 294-298. (Qu Y, Li W J, Zhou L B,et al. Research and application of mutagenic effects in plants irradiated by heavy ion beams［J］.Nuclear Physics Review, 2007, 24(4): 294-298.)
［62］周利斌, 李文建, 曲颖, 等. 重离子束辐照育种研究进展及发展趋势［J］. 原子核物理评论, 2008, 25(2): 165-170.(Zhou L B, Li W J, Qu Y , et al. Progress and tendency in heavy ion irradiation mutation breeding［J］. Nuclear Physics Review, 2007, 24(4): 294-298.)
［63］刘建光, 王永强, 赵贵元, 等. 重离子辐照诱变育种应用及其生物学效应研究进展［J］. 作物杂志, 2016, 3(3): 12-16. (Liu J G, Wang Y Q, Zhao G Y, et al. Advances of heavy ion beam application in plant breeding and their biological effect［J］.Crops, 2016, 3(3): 12-16.)
［64］杨陶丽薇,刘瑞媛,顾文婷,等. 碳离子辐照对甜高粱糖分与蔗糖代谢相关酶活力的影响［J］. 辐射研究与辐射工艺学报, 2015, 33(5): 44-51.（Yang-Tao L W ,Liu R Y, Gu W T, et al. Effects on the sugar components and related enzyme activity of sucrose metabolism by carbon ion irradiation in sweet sorghum ［J］.Journal of Radiation Research and Radiation Processing, 2015, 33(5): 44-51.）
［65］冯学金, 郭秀娟, 杨建春, 等. 诱变技术在亚麻育种中的应用［J］. 核农学报, 2017, 31(7): 1310-1316.(Feng X J, Guo X J, Yang J C ,et al. Application of mutation technology in flax breeding［J］.Journal of Nuclear Agricultural Sciences, 2017, 31(7): 1310-1316.)
［66］Ubonprasert B, Srinivers P, Lamseejan S, et al. Effects of gamma irradiation of soybean cotyledon explants on embryo induction and regeneration in a somatic embryo cycling system［J］. Journal of Breeding & Genetics,1998,30(1):19-24.
［67］余丽霞, 李文建, 杜艳, 等. 碳离子束辐照大豆当代诱变效应及褐皮突变体的初步研究［J］. 辐射研究与辐射工艺学报, 2014, 32(2): 53-59. (Yu L X, Li W J, Du Y, et al. Preliminary study on current mutagenic effects and brown seed mutant of soybean induced by carbon ion irradiation［J］. Journal of Radiation Research and Radiation Processing, 2014, 32(2): 53-59.)
［68］张秋英,余丽霞,李彦生,等.重离子束辐照大豆籽粒当代效应的初步研究［J］.大豆科学,2013,32(5):587-590.(Zhang Q Y, Yu L X, Li Y S, et al. Preliminary investigation of acceptable heavy ion beam irradiation dosage treated to soybean (Glycine max L.) seed［J］. Soybean Science, 2013,32(5):587-590.)
［69］曲桂芹,张贤泽,霍俊伟. 影响大豆体细胞胚诱导因素的研究［J］. 植物研究,2001(2):210-214,323.(Qu G Q, Zhang X Z, Huo J W. Studies on somatic studies on somatic embryos induction in soybean (Glycine Max L.)［J］.Bulletin of Botanical Research, 2001(2):210-214,323.)
［70］周思君,尹光初,雷勃钧,等. 大豆体细胞胚胎发生影响因素的研究［J］. 植物学通报,1992(2):38-43.(Zhou S J, Yin G C, Lei B J, et al. Studies on the influential factors of the somatic embryogenesis of soybean［J］. Chinese Bulletin of Botany, 1992(2):38-43.)
［71］吴超,武天龙.大豆未成熟子叶体细胞再生及相关农艺性状影响因子的分析［J］.大豆科学,2004,23(1):22-25.(Wu C,Wu T L. Somatic embryogenesis from immature cotyledons and analysis of correlative agronomic characters［J］.Soybean Science, 2004,23(1):22-25.)

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[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(03):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(03):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(03):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(03):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(03):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(03):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(03):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(03):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

收稿日期：2018-01-24

基金项目：哈尔滨市应用技术研究与开发项目(2017RAQYJ064)；黑龙江省农业科技创新工程(2017SJ011)。
第一作者简介：薛永国(1981-)，男，博士，助理研究员，主要从事大豆遗传育种研究。E-mail:xyg81@126.com。
通讯简介：栾晓燕(1964-)，女，硕士，研究员，主要从事大豆遗传育种研究。E-mail：luanxiaoyan1201@163.com。

更新日期/Last Update: 2018-06-08