PDF下载 +分享

微信公众号：大豆科学

[1]孔令剑,朱倩,单玉姿,等.蔗糖对低磷胁迫条件下大豆苗期根系形态和物质积累的影响[J].大豆科学,2018,37(02):239-245.[doi:10.11861/j.issn.1000-9841.2018.02.023]
　KONG Ling-jian,ZHU Qian,SHAN Yu-zi,et al.Effect of Sucrose on Root Morphology and Substance Accumulation at Soybean Seedling Stage under Low Phosphorus Stress[J].Soybean Science,2018,37(02):239-245.[doi:10.11861/j.issn.1000-9841.2018.02.023]

点击复制

蔗糖对低磷胁迫条件下大豆苗期根系形态和物质积累的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷期数: 2018年02期页码: 239-245 栏目: 出版日期: 2018-03-20

Title:: Effect of Sucrose on Root Morphology and Substance Accumulation at Soybean Seedling Stage under Low Phosphorus Stress

作者:: 孔令剑; 朱倩; 单玉姿; 谢甫绨; 王海英; 张惠君; 赵明哲; 敖雪; (沈阳农业大学农学院，辽宁沈阳 110866)

Author(s):: KONG Ling-jian; ZHU Qian; SHAN Yu-zi; XIE Fu-ti; WANG Hai-ying ; ZHANG Hui-jun; ZHAO Ming-zhe; AO Xue; (College of Agronomy, Shenyang Agricultural University, Shenyang 110866，China)

关键词:: 大豆; 低磷胁迫; 根系; 蔗糖; 物质积累

Keywords:: Soybean; Low phosphorus stress; Root; Sucrose; Biomass accumulation

分类号:: S565.1

DOI:: 10.11861/j.issn.1000-9841.2018.02.023

文献标志码:: A

摘要:: 为了研究施加外源蔗糖对低磷胁迫下苗期大豆的影响，以两个不同磷效率大豆为试验材料，设置 3个磷浓度水平（0，0.05和0.50 mmol?L-1）和2个蔗糖浓度水平（0%和1%）处理，测定幼苗时期不同处理下大豆根系形态、蔗糖含量、磷积累和植株生物量的积累特征。结果显示：加入蔗糖后，磷高效品种的根尖数由平均高于磷低效大豆的28.5%升高至88.9%，蔗糖含量、根系磷积累以及根系生物量平均升高15.5%、7.2%和26.6%；而磷低效品种蔗糖含量、根系磷积累以及根系生物量平均升高18.4%、31.7%和50.6%。表明蔗糖处理对磷低效品种在生物量积累以及养分吸收方面影响更显著。

Abstract:: In order to study the effect of exogenous sucrose on the response mechanism of soybean seedling to low phosphorus stress, this study was conducted with two different phosphorus(P) efficient soybeans, three P concentrations (0, 0.05 and 0.50 mmol?L-1) and two sucrose levels (0% and 1%), to investigate the traits of root morphology, sucrose content, P accumulation and plant biomass at soybean seedling stage. The results showed: For the low P efficient soybean, sucrose application enlarged the increment in root tip numbers of the high P efficient soybean than the low P efficient soybean, from 28.5% to 88.9%, compared with no sucrose application. Sucrose application increased the sucrose content, root phosphorus accumulation and the root biomass averagely by 15.5%, 7.2% and 26.6%, respectively. For the low P efficient soybean, the sucrose content, root phosphorus accumulation and the root biomass increased 18.4%, 31.7%, and50.6%, respectively. This result indicated that sucrose application affacted more significantly on biomass accumulation and nutrient absorption for the low P efficient soybeans.

参考文献/References:

［1］敖雪,谢甫绨,张惠君,等. 磷素处理对不同磷效率基因型大豆根系性状的影响［J］. 大豆科学,2008,27(5):787-791. (Ao X, Xie F T, Zhang H J, et al. Effect of phosphorus on root traits of soybean cultivars with different phosphorus efficiencies［J］. Soybean Science,2008,27(5):787-791.)

［2］Tara S, Niels E N. Root traits as tools for creating phosphorus efficient crop varieties［J］. Plant and Soil, 2004,260:47-57.

［3］Laurence L, Judith W, Marjorie P, et al. Oxidative pentose phosphate pathway-dependent sugar sensing as a mechanismfor regulation of root ion transporters by photosynthesis［J］.Plant Physiology, 2008, 146(4): 2036-2053.

［4］Hammond J P, White P J. Sucrose transport in the phloem: Integrating root responses to phosphorus starvation［J］.Journal of Experimental Botany, 2008, 59(1): 93-95.

［5］曹敏建, 佟占昌,韩明祺,等.磷高效利用的大豆遗传资源的筛选与评价［J］.作物杂志, 2001(4):22-24. (Cao M J, Tong Z C, Han M Q, et al. Screening and evaluation of soybean genetic resources with high phosphorus efficiency［J］. Crops, 2001(4):22-24.)

［6］曹爱琴,严小龙.不同供磷条件下大豆根构型的适应性变化［J］.华南农业大学学报,2000,22(1):92 .(Cao A Q, Yan X L. Adaptation of soybean root architecture under different P conditions［J］.Journal of South China Agricultural University,2000,22(1):92.)

［7］Junqi L, Deborah A S, Bruna B, et al. Signaling of phosphorus deficiency-induced gene expression in white lupin requires sugar and phloem transport［J］. The Plant Journal, 2005, 41(2): 257-268.

［8］Lei M G, Liu Y D, Zhang B C, et al. Genetic and genomic evidence that sucrose is a global regulator of plant responses to phosphate starvation in Arabidopsis［J］. Plant Physiology,2011, 156(3): 1116-1130.

［9］Zhou K Q, Masumi Y, Mitsuru O, et al. Sugar signalling mediates cluster root formation and phosphorus starvation-induced gene expression in white lupin［J］. Journal of Experimental Botany, 2008, 59(10): 2749-2756.

［10］苏军,张武君,杜琳,等.磷胁迫下蔗糖对水稻苗期根适应性和磷酸转运蛋白基因表达的影响［J］.中国生态农业学报,2014, 22(11): 1334-1340.(Su J, Zhang W J, Du L, et al. Effects of sucrose on rice root adaptability and phosphate transporter gene expression under phosphorus stress［J］.Chinese Journal of Eco-Agriculture,2014, 22(11): 1334-1340.)?

［11］吴俊江,钟鹏,刘丽君,等.不同大豆基因型耐低磷能力的评价［J］.大豆科学, 2008,27(6):983-987. (Wu J J, Zhong P, Liu L J, et al. Evaluationon the low phosphorous tolerance of different soybean genotypes［J］. Soybean Science, 2008,27(6):983-987.)

［12］李志刚, 谢甫绨, 宋书宏.大豆高效利用磷素基因型的筛选［J］.中国农学通报, 2004, 20(5): 126-129.(Li Z G, Xie F T, Song S H. The selection of high phosphorus using efficient soybean genotype［J］. Chinese Agriculture Science Bulletin, 2004，20(5):126-129.)

［13］张志良.植物生理学实验指导［M］.北京: 高等教育出版社, 2005:128-129. (Zhang Z L. Experimental guidance of plant physiology［M］. Beijing: Higher Education Press, 2005:128-129.)

［14］徐青萍,罗超云,廖红,等.大豆不同品种对磷胁迫反应的研究［J］.大豆科学,2003,22(2): 108-114. (Xu Q P, Luo C Y,Liao H, et al. Study on the response of soybean varieties to P deficiency［J］. Soybean Science,2003,22(2): 108-114.)

［15］李庆逵. 现代磷肥研究的进展［J］.土壤学进展,1986(2): 1-7. (Li Q K. Progress in the research of modern phosphate fertilizer［J］. Progress in Soil Science, 1986(2): 1-7.)

［16］庞欣, 李春俭, 张福锁.部分根系供磷对小麦幼苗生长及同化物分配的影响［J］.作物学报,2006,32(6):719-724.(Pang X, Li C J, Zhang F S. Effect of partial-root supply of P nutrient on the growth and assimilate distribution of wheat seedlings［J］. Acta Agronnica Sinica, 2006,32(6):719-724.)

［17］Mohammad R B M, Wim V E. Sugars and plant innate immunity［J］.Journal of Experimental Botany,2012,63(11):3989-3998.

［18］Laurence L, Xavier G, Miguel C, et al. Regulation of root ion transporters by photosynthesis: Functional importance and relation with hexokinase［J］. The Plant Cell, 2003,15(9):2218-2232.

［19］Athikkattuvalasu S K, Deepa K V, Ajay J, et al. Phosphate starvation responses are mediated by sugar signaling in Arabidopsis［J］.Planta,2007,225(4): 907-918.

［20］马春梅, 郭海龙, 龚振平, 等.不同基因型大豆糖分积累规律的研究—(II)蔗糖含量积累规律研究［J］.作物杂志, 2011(1):25-29.(Ma C M, Guo H L, Gong Z P, et al. Sugar accumulation in soybean among different genotypes—(II)Study on the accumulation rule of sucrose content［J］. Crops, 2011(1):25-29.)

［21］马春梅, 郭海龙, 龚振平, 等.不同基因型大豆糖分积累规律的研究(I)—可溶性总糖含量积累规律的研究［J］.作物杂志, 2010(4):65-69. (Ma C M, Guo H L, Gong Z P, et al. Sugar accumulation among different soybean genotype (I) —Accumulationof total soluble sugar content［J］.Crops,2010(4):65-69.)

［22］张彦丽. 不同磷效率大豆基因型根形态构型对低磷胁迫的响应［J］.中国农学通报,2010, 26(14): 182-185. (Zhang Y L. Response of low-phosphorus stress on root morphology and architecture of soybean genotype with different phosphorus efficiency［J］. Chinese Agricultural Science Bulletin ,2010, 26(14): 182-185.)

［23］Zhu Y Y, Yan F, Christian Z, et al. A link between citrate and proton release by proteoid roots of White Lupin(Lupinus albus L.) grown under phosphorus-deficient conditions［J］. Plant and Cell Physiology, 2005,46(6): 892-901.

［24］李锋,潘晓华.植物适应缺磷胁迫的根系形态及生理特征研究进展［J］.中国农学通报, 2002, 18(5): 65-69. (Li F, Pan X H. The research development of morphological and physiological characteristics of plant root system under phosphorus deficiency［J］. Chinese Agricultural Science Bulletin,2002, 18(5): 65-69.)

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(02):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(02):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(02):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(02):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(02):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(02):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(02):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(02):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(02):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(02):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]董薇,练云,余永亮,等.大豆磷胁迫响应研究进展[J].大豆科学,2012,31(01):135.[doi:10.3969/j.issn.1000-9841.2012.01.031]
　DONG Wei,LIAN Yun,YU Yong-liang,et al.Advances in Low Phosphorus Stress on Soybean[J].Soybean Science,2012,31(02):135.[doi:10.3969/j.issn.1000-9841.2012.01.031]
[12]王树起,韩晓增,乔云发,等.缺磷胁迫对大豆根瘤生长和结瘤固氮的影响[J].大豆科学,2009,28(06):1000.[doi:10.11861/j.issn.1000-9841.2009.06.1000]
　WANG Shu-qi,HAN Xiao-zeng,QIAO Yun-fa,et al.Nodule Growth，Nodulation and Nitrogen Fixation in Soybean(Glycine Max L.）as Affected by P Deficiency Stress[J].Soybean Science,2009,28(02):1000.[doi:10.11861/j.issn.1000-9841.2009.06.1000]
[13]吴俊江,钟鹏,刘丽君,等.不同大豆基因型耐低磷能力的评价[J].大豆科学,2008,27(06):983.[doi:10.11861/j.issn.1000-9841.2008.06.0983]
　WU Jun-jiang,ZHONG Peng,LIU Li-jun,et al.Evaluation on the Low Phosphorous Tolerance of Different Soybean Genotypes[J].Soybean Science,2008,27(02):983.[doi:10.11861/j.issn.1000-9841.2008.06.0983]
[14]吴俊江,刘丽君,钟鹏,等.低磷胁迫对不同基因型大豆保护酶活性的影响[J].大豆科学,2008,27(03):437.[doi:10.11861/j.issn.1000-9841.2008.03.0437]
　WU Jun-jiang,LIU Li-jun,ZHONG Peng,et al.Effects of Low Phosphorus Stress on Activities of Cell Defense Enzymes of Different P-efficiency Soybean[J].Soybean Science,2008,27(02):437.[doi:10.11861/j.issn.1000-9841.2008.03.0437]
[15]姚敏磊,张璟曜,周汐,等.大豆响应低磷胁迫的数字基因表达谱分析[J].大豆科学,2016,35(02):213.[doi:10.11861/j.issn.1000-9841.2016.02.0213]
　YAO Min-lei,ZHANG Jing-yao,ZHOU Xi,et al.The Digital Gene Expression Profiling Analysis of Genes in Response to Low Phosphorus Stress in Soybean[J].Soybean Science,2016,35(02):213.[doi:10.11861/j.issn.1000-9841.2016.02.0213]
[16]孙崇源,崔瑞凡,王瑞阳,等.大豆ERF家族基因生物信息学分析及其对低磷胁迫的响应[J].大豆科学,2021,40(06):748.[doi:10.11861/j.issn.1000-9841.2021.06.0748]
　SUN Chong-yuan,CUI Rui-fan,WANG Rui-yang,et al.Bioinformatics Analysis on Soybean ERF Family Genes and Their Response to Low Phosphorus Stress[J].Soybean Science,2021,40(02):748.[doi:10.11861/j.issn.1000-9841.2021.06.0748]

备注/Memo

收稿日期：2018-01-26

基金项目：辽宁省高等学校优秀人才支持计划资助（LJQ2015097）；辽宁省自然科学基金重点项目（201701526）；国家重点研发计划（2017YFD0101306-04）。

第一作者简介：孔令剑（1993-），男，硕士，主要从事大豆产量营养生理研究。E-mail：18240418826@163.com。

通讯作者：敖雪（1979-），女，博士，副教授，主要从事作物营养逆境生理研究。E-mail：gebywka@sohu.com。

更新日期/Last Update: 2018-04-02