[1]张春宝,林春晶,赵丽梅,等.分子标记研究野生大豆遗传多样性进展[J].大豆科学,2018,37(01):8-13.[doi:10.11861/j.issn.1000-9841.2018.01.0008]
　ZHANG Chun-bao,LIN Chun-jing,ZHAO Li-mei,et al.Advances in Evaluation of Genetic Diversity in Wild Soybean by Molecular Markers[J].Soybean Science,2018,37(01):8-13.[doi:10.11861/j.issn.1000-9841.2018.01.0008]
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分子标记研究野生大豆遗传多样性进展

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年01期 页码: 8-13 栏目: 出版日期: 2018-01-20
Title:
Advances in Evaluation of Genetic Diversity in Wild Soybean by Molecular Markers
作者:
张春宝 1; 林春晶 1; 赵丽梅 1; 董英山 1; 赵洪锟 2; 王玉民 1
（1.吉林省农业科学院 大豆研究所，吉林 长春 130033； 2.吉林省农业科学院 作物资源研究所，吉林 公主岭 136100）
Author(s):
ZHANG Chun-bao 1;  LIN Chun-jing 1;  ZHAO Li-mei 1;  DONG Ying-shan 1;  ZHAO Hong-kun 2;  WANG Yu-min 1
（1.Soybean Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033, China; 2.Crop Resources Research Institute, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China）
关键词:
野生大豆; 分子标记; 遗传多样性
Keywords:
Wild soybean;  Molecular marker;  Genetic Diversity
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2018.01.0008
文献标志码:
A
摘要:
野生大豆是栽培大豆的近缘野生种，是大豆遗传改良的重要基因源。野生大豆的遗传多样性研究对野生大豆资源的收集、保存、保护和利用具有重要意义。本文从种质库资源的多样性，不同国家野生大豆资源的多样性，野生大豆种群的多样性和野生大豆种群间的遗传分化几个方面，总结了利用RAPD、AFLP和SSR等DNA分子标记技术对野生大豆资源和种群进行遗传多样性研究的进展。
Abstract:
Wild soybean (Glycine soja) is the ancestor of cultivated soybean (Glycine max) and is an important gene pool for soybean genetic improvement. The evaluation of the genetic diversity of wild soybean is of great importance for the collection, preservation, conservation and utilization of wild soybean resources. This article reviewed the advances of using molecular markers, such as RAPD, AFLP and SSR markers, in evaluation the genetic diversity of wild soybean from 4 aspects, included the genetic diversity of wild soybean from the genebank, the genetic diversity of wild soybean from different countries, the genetic diversity of wild soybean populations, and the genetic differentiation between populations of wild soybean.

参考文献/References:

［1］ 丁振麟. 野生大豆与栽培大豆之遗传研究［J］. 中华农学会报, 1945(182): 4778. (Ding Z L. Genetic study on wild and cultivated soybean［J］. Journal of the agricultural association of china, 1945(182): 4778.)

［2］ 徐豹, 路琴华. 大豆生态研究Ⅰ中国不同纬度野生大豆的光温生态分析［J］. 大豆科学, 1983, 2(3): 155-168. (Xu B, Lu Q H. Soybean ecologyⅠeffect of photoperiod and day/night temperature on development of wild soybean in China［J］. Soybean Science, 1983, 2(3): 155-168.)
［3］ 赵洪锟, 庄炳昌, 王玉民, 等. 中国不同纬度野生大豆和栽培大豆AFLP分析［J］. 高技术通讯, 2000（7）:32-35. (Zhao H K, Zhuang B C, Wang Y M, et al. AFLP analysis of wild (G.soja) and cultivated soybean (G.max) in China［J］. High Technology Letters, 2000(7): 32-35.)
［4］ Li Z, Nelson R L. RAPD marker diversity among cultivated and wild soybean accessions from four Chinese provinces［J］. Crop Science, 2002, 42: 1737-1744.
［5］ An W, Zhao H K, Dong Y S, et al. Genetic diversity in annual wild soybean (Glycine soja Sieb. et. zucc.) and cultivated soybean (G.max.Merr) from different latitudes in China［J］. Pakistan Journal of Botany, 2009, 41(5): 2229-2242.
［6］ Lam H M, Xu X, Liu X, et al. Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection［J］. Nature Genetics, 2010, 42: 1053-1059.
［7］ 林红, 齐宁, 李向华, 等. 黑龙江省野生大豆资源考察研究［J］. 中国油料作物学报, 2006, 28(4): 427-430. (Lin H, Qi N, Li X H, et al. New progress on wild soybean survey in Heilongjiang province［J］. Chinese Journal of Oil Crop Sciences, 2006, 28 (4): 427-430.)
［8］ 李建东, 燕雪飞, 董思言, 等. 辽宁省野生大豆种质资源的SSR遗传多样性分析［J］. 大豆科学, 2010, 29(1): 28-32. (Li J D, Yan X F, Dong S Y, et al. Analysis of genetic diversity of Glycine soja?germplasm resources in Liaoning province［J］. Soybean Science, 2010, 29(1): 28-32.)
［9］ 王果, 胡正, 张保缺, 等. 山西省野生大豆资源遗传多样性分析［J］. 中国农业科学, 2008, 41(7): 2182-2190. (Wang G, Hu Z, Zhang B Q, et al. Genetic diversity analysis of Shanxi’s wild soybean (f Glycine soja )［J］. Chinese Agricultural Sciences, 2008, 41(7): 2182-2190.)
［10］陈辉, 张磊, 张文明, 等. 安徽省新收集野生大豆种质资源的SSR分析［J］. 中国农学通报, 2008, 24(3): 345-349. (Chen H, Zhang L, Zhang W M, et al. SSR analysis of newly collected wild soybean germplasms from Anhui province［J］. Chinese Agricultural Science Bulletin, 2008, 24(3): 345-349.)
［11］程春明, 杨存义, 马启彬, 等. 江西野生大豆遗传多样性分析［J］. 植物遗传资源学报, 2011, 12(6): 928-933, 94. (Cheng C M, Yang C Y, Ma Q B, et al. Genetic diversity analysis of wild soybean resources in Jiangxi［J］. Journal of Plant Genetic Resources, 2011, 12(6): 928-933, 94.)
［12］魏苗, 李建东, 燕雪飞, 等. 中国东北野生大豆SSR遗传多样性及亲缘关系分析［J］.大豆科学, 2011, 30(3): 388-392, 396. (Wei M, Li J D, Yan X F, et al. Analysis of genetic diversity and relationship of Glycine soja in Northeast China［J］. Soybean Science, 2011, 30(3): 388-392, 396.)
［13］刘亚男, 李向华, 王克晶. 国家基因库野生大豆微核心样本遗传变异性的SSR标记分析 ［J］. 植物遗传资源学报, 2009, 10(2): 211-217. (Liu Y N, Li X H, Wang K J. Analysis of the genetic variability for the mini-core collection of Chinese wild soybean ( Glycine soja ) collection in the national gene bank based on SSR markers［J］. Journal of Plant Genetic Resources, 2009, 10(2): 211-217.)
［14］文自翔, 赵团结, 丁艳来, 等. 中国栽培及野生大豆的遗传多样性、地理分化和演化关系研究［J］. 科学通报, 2009, 54(21): 3301-3310. (Wen Z X, Zhao T J, Ding Y L, et al. Genetic diversity, geographic differentiation and evolutionary relationship among ecotypes of Glycine max?and G. soja?in China［J］. Chinese Science Bulletin, 2009, 54(21):3301-3310.)
［15］Li X H, Wang K J, Jia J Z. Genetic diversity and differentiation of Chinese wild soybean germplasm (G. soja Sieb. & Zucc.) in geographical scale revealed by SSR markers［J］. Plant Breeding, 2009, 128: 658-664.
［16］董英山, 庄炳昌, 赵丽梅, 等. 中国野生大豆遗传多样性中心［J］.作物学报, 2000, 26(5): 521-527. (Dong Y S, Zhuang B C, Zhao L M, et al. The Genetic diversity centers of annual wild soybean in China ［J］. Acta Agronomica Sinica, 2000, 26(5): 521-527.)
［17］Wang K, Takahata Y. A preliminary comparative evaluation of genetic diversity between Chinese and Japanese wild soybean (G. soja) germplasm pools using SSR markers［J］. Genetic Resources and Crop Evolution, 2007, 54(1): 157-165.
［18］张小明, 刘丽君, 唐晓飞, 等. 中俄大豆种质遗传多样性分析［J］. 大豆科学, 2008, 27(1): 15-20. (Zhang X M, Liu L J, Tang X F, et al. Genetic diversity of soybean germplasm in Russia and China ［J］. Soybean Science, 2008, 27(1):15-20.)
［19］Lee J, Yu J, Hwang Y, et al. Genetic diversity of wild soybean (Glycine soja Sieb. and Zucc.) accessions from South Korea and other countries［J］. Crop Science, 2008, 48(2): 606-616.
［20］朴向民, 张圣珍, 许建, 等. 中国吉林省和韩国野生大豆的遗传多样性及遗传关系分析 ［J］. 大豆科学, 2009, 28(2): 181-185. (Piao X M, Zhang S Z, Xu J, et al. Genetic diversity of annual wild soybean (G. soja) between China Jilin province and Korean［J］. Soybean Science, 2009, 28(2): 181-185.)
［21］Huenneke L F. Ecological implications of genetic variation in plant populations//Falk D A, Holsinger K E. Genetics and conservation of rare plants［M］. New York: Oxford University Press, 1991: 31-44.
［22］周晓馥, 庄炳昌, 王玉民, 等. 利用RAPD与SSR技术进行野生大豆种群内分化的研究 ［J］.中国生态农业学报, 2002, 10(4): 6-9. (Zhou X F, Zhuang B C, Wang Y M, et al. Population differentiation of wild soybean based on the RAPD and SSR analysis［J］. Chinese Journal of Eco-Agriculture, 2002, 10(4):6-9.)
［23］孙晓环, 刘晓冬, 赵洪锟, 等. 吉林省龙井保护区野生大豆居群遗传多样性的研究［J］. 吉林农业科学, 2010, 35(2):1-4. (Sun X H, Liu X D, Zhao H K, et al. Diversity of a population of wild soybean (G. soja) growing in Longjing conserved region of Jilin Province ［J］. Journal of Jilin Agricultural Sciences, 2010, 35(2):1-4.)
［24］王丹, 乔亚科, 韩粉霞, 等. 河北东部沿海地区野生大豆SSR多样性分析［J］. 大豆科学, 2010, 29(4): 555-558. (Wang D, Qiao Y K, Han F X, et al. Genetic diversity of G. soja?in eastern coastal area of Hebei province［J］. Soybean Science, 2010, 29(4): 555-558.)
［25］赵青松, 陈振伦, 赵云云, 等. 广东5个野生大豆居群的遗传多样性分析［J］. 西北植物学报, 2013, 33(9): 1768-1774. (Zhao Q S, Chen Z L, Zhao Y Y, et al. Genetic diversity of 5 wild soybean populations from Guangdong［J］. Acta Botanica Boreali-Occidentalia Sinica, 2013, 33(9): 1768-1774.)
［26］府宇雷, 钱吉, 马玉虹，等. 不同尺度下野大豆种群的遗传分化［J］. 生态学报, 2002, 22(2): 176-184. (Fu Y L, Qian J, Ma Y H, et al. Genetic differentiation research on populations of wild soybeans in different scales［J］. Acta Ecologica Sinica, 2002, 22(2): 176-184.)
［27］府宇雷, 钱吉, 张美云, 等. 金华地区野生大豆小种群的分子生态学研究［J］. 复旦学报(自然科学版), 1999, 38(5): 584-586. (Fu Y L, Qian J, Zhang M Y, et al. Molecular-Ecology study on sub-population of wild soybeans within jinhua district［J］. Journal of Fudan University(Natural Science), 1999, 38(5): 584-586.)
［28］关荣霞, 刘秀敏, 常汝镇, 等. 辽宁新宾县原位保护区野生大豆(Glycine soja Sieb. & Zucc.)遗传多样性分析［J］. 高技术通讯, 2006, 16(1): 67-72. (Guan R X, Liu X M, Chang R Z, et al. Genetic diversity analysis of wild soybean (G. soja Sieb. & Zucc.) from in-situ conserved population in Xinbin county of Liaoning province［J］. Chinese High Technology Letters, 2006, 16(1): 67-72.)
［29］钱吉, 陈庠, 郑师章, 等. 不同纬度野生大豆种群间的遗传变异［J］. 复旦学报(自然科学版), 1998, 37(2):208-212. (Qian J, Chen Y, Zheng S Z, et al. Genetic variations among populations of wild soybeans from different latitude［J］. Journal of Fudan University (Natural Science), 1998, 37(2):208-212.)
［30］徐立恒, 李向华. SSR标记对野生大豆种群遗传结构的研究［J］. 大豆科学, 2011, 30(1): 41-45. (Xu L H, Li X H. Analysis on genetic structure of wild soybeans populations by SSR markers［J］. Soybean Science, 2011, 30(1):41-45.)
［31］He S, Wang Y, Volis S,et al. Genetic diversity and population structure: implications for conservation of wild soybean(G. soja Sieb. and Zucc.) based on nuclear and chloroplast microsatellite variation［J］. International Journal of Molecular Sciences, 2012, 13: 11262-12608.
［32］Schmutz J, Cannon S B, Schlueter J, et al. Genome sequence of the palaeopolyploid soybean ［J］. Nature, 2010, 463(7278): 178-183.
［33］KimM Y, Lee S, Van K, et al. Whole-genome sequencing and intensive analysis of the undomesticated soybean(G. soja Sieb. and Zucc.) genome［J］. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(51): 22032-22037.
［34］Li Y H, Zhou G, Ma J, et al. De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits［J］. Nature Biotechnology, 2014, 32(10): 1045-1052.
［35］Zhou Z K, Jiang Y, Wang Z, et al. Resequencing 302 wild and cultivated accessions identifies genes related to domestication and improvement in soybean［J］. Nature Biotechnology, 2015, 33(4): 408-414.
［36］Sun L, Miao Z, Cai C,et al. GmHs1-1, encoding a calcineurin-like protein, controls hard-seededness in soybean［J］. Nature Genetics, 2015, 47(8): 939-943.
［37］Ge L, Yu J, Wang H, et al. Increasing seed size and quality by manipulating BIG SEEDS1 in legume species［J］. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113(44): 12414-12419.
［38］Lu X, Xiong Q, Cheng T, et al. A PP2C-1 allele underlying a quantitative trait locus enhances soybean 100-seed weight［J］. Molecular Plant, 2017, 10(5): 670-684.

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备注/Memo

收稿日期：2017-07-17

基金项目：吉林省农业科技创新工程项目（CXGC2017JQ018，CXGC2017ZD014）；国家自然科学基金（31300282）；农业部野生资源保护与利用项目（09162130135252028）。
第一作者简介：张春宝（1980-），男，博士，副研究员，主要从事大豆遗传育种。E-mail:cbzhang@126.com。
通讯作者：赵洪锟（1974-），女，博士，研究员，主要从事野生大豆资源研究。E-mail：zhaohk99@126.com；
? ? ? ? ? ? ? ? ? ? ?王玉民（1968-），男，博士，研究员，主要从事大豆种质资源研究。E-mail：wangym@cjaas.com。

更新日期/Last Update: 2018-03-09