[1]李悦,张宇航,李冬梅,等.大豆GmABCG40基因的功能预测及表达分析[J].大豆科学,2017,36(04):502-507.[doi:10.11861/j.issn.1000-9841.2017.04.0502]
　LI Yue,ZHANG Yu-hang,LI Dong-mei,et al.Functional Prediction and Expression Analysis of GmABCG40[J].Soybean Science,2017,36(04):502-507.[doi:10.11861/j.issn.1000-9841.2017.04.0502]
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大豆GmABCG40基因的功能预测及表达分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年04期 页码: 502-507 栏目: 出版日期: 2017-07-20
Title:
Functional Prediction and Expression Analysis of GmABCG40
作者:
李悦1; 张宇航1; 李冬梅1; 王涛1; 陈薇1; 王珣2; 李永光1; 李文滨1
（1.东北农业大学 农学院/大豆生物学教育部重点实验室，黑龙江 哈尔滨 150030； 2.黑龙江省农业科学院 生物技术研究所，黑龙江 哈尔滨 150030）
Author(s):
LI Yue1;  ZHANG Yu-hang1;  LI Dong-mei1;  WANG Tao1;  CHEN Wei1;  WANG Xun2;  LI Yong-guang1;  LI Wen-bin1
（Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Soybean Biology and Genetics Breeding of Chinese Agriculture Ministry/College of Agriculture of Northeast Agricultural University，Harbin 150030，China）
关键词:
大豆; ABCG转运蛋白; 表达分析; 功能; 生物信息学
Keywords:
Soybean;  ABCG transporters;  Expression analysis;  Function;  Bioinformatics.
DOI:
10.11861/j.issn.1000-9841.2017.04.0502
文献标志码:
A
摘要:
通过在大豆基因组数据库中检索拟南芥AtABCG40在大豆中的同源基因，获得了GmABCG40基因序列。通过对GmABCG40基因编码的氨基酸序列及启动子序列进行生物信息学分析，结果表明：GmABCG40基因CDS序列全长4 284 bp, 编码1 427个氨基酸。GmABCG40编码的蛋白为疏水性蛋白，具有多个N-糖基化位点、激酶磷酸化位点、N-豆蔻酰化位点、2个ATP/GTP结合位点基序A和1个速激肽家族信号。结构域分析表明GmABCG40含有2个核苷酸结合域与2个跨膜结构域，形成NBD1-TMD1-NBD2-TMD2结构，属于ABCG亚家族的成员。GmABCG40预测的启动子区域含有与激素、胁迫、光应答、胚乳表达和转录因子结合相关的顺式作用元件。系统进化分析表明GmABCG40与菜豆、红豆、木豆、百脉根等豆科植物亲缘关系较近。组织特异性表达分析结果显示GmDABCG40在叶片中表达量最低，在根中表达量最高，推测其可能参与根中ABA的转运过程。
Abstract:
GmABCG40 gene sequence was obtained by searching the homologous gene of AtABCG40 in the soybean genome database. Bioinformatics analysis of amino acid sequence encoded by GmABCG40 and promoter sequence showed that the full-length CDS sequence of GmABCG40 was 4 284 bp, encoding a 1 427 amino acids protein GmABCG40 was a hydrophobic protein, possessed multiple N-glycosylation sites,kinase phosphorylation sites, N-myristoylation sites, two ATP/AGP binding site motif A and one tachykinin family signal. Structure domains analysis indicated that GmABCG40 contained two nucleotide binding domains and two trans membrane domains, which constituted NBD1-TMD1-NBD2-TMD2 structure and belonging to the ABCG subfamily.Cis-elements associated with hormones, stresses, light responses, endosperm expression and transcription factors binding were existed in the predicted promoter region.Phylogenetic analysis results suggested that GmABCG40 had the highest genetic relationship with leguminous plants such as Phaseolus vulgaris, Vigna angularis, Cajanus cajan and Lotus japonicus.Results of tissue specific expression analysis revealed that expression level of GmABCG40 was lowest in leaves, however, highest in roots, it was speculated to participate in the ABA transport process in roots.

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备注/Memo

基金项目：黑龙江普通本科高等学校青年创新人才培养计划（UNPYSCT-2016005）；抗逆转基因大豆新品培育（2016ZX0804002）。

第一作者简介：李悦（1992-），女，硕士，主要从事作物遗传育种研究。E-mail:364544346@qq.com。
通讯作者：李文滨（1958-），男，教授，博导，主要从事作物遗传育种研究。E-mail:wenbinli@neau.edu.cn。

更新日期/Last Update: 2017-08-14