[1]商璐,谭洪鹤,洪慧龙,等.抗草甘膦栽培大豆种质挖掘及抗性生理研究[J].大豆科学,2017,36(01):60-67.[doi:10.11861/j.issn.1000-9841.2017.01.0060]
　SHANG Lu,TAN Hong-he,HONG Hui-long,et al.Glyphosate Resistant Screening of Different Soybean Varieties and Its Resistance Mechanism[J].Soybean Science,2017,36(01):60-67.[doi:10.11861/j.issn.1000-9841.2017.01.0060]
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抗草甘膦栽培大豆种质挖掘及抗性生理研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年01期 页码: 60-67 栏目: 出版日期: 2017-01-20
Title:
Glyphosate Resistant Screening of Different Soybean Varieties and Its Resistance Mechanism
作者:
商璐; 谭洪鹤; 洪慧龙; 陶波; 高山; 隋冰
东北农业大学 农学院，黑龙江 哈尔滨 150030
Author(s):
SHANG Lu;  TAN Hong-he;  HONG Hui-long;  TAO Bo;  GAO Shan;  SUI Bing
College of Agriculture, Northeast Agricultural University, Harbin 150030, China
关键词:
栽培大豆; 草甘膦抗性; 莽草酸; 叶绿素; SOD
Keywords:
Cultivated soybean;  Glyphosate resistance;  Shikimic acid;  Chlorophyll;  SOD
DOI:
10.11861/j.issn.1000-9841.2017.01.0060
文献标志码:
A
摘要:
利用田间试验结合生物化学分析研究了不同的栽培大豆品系对草甘膦的抗性，试验结果显示：在草甘膦有效剂量为0.31～0.92 ·hm-2时，不同栽培大豆品种对草甘膦抗性存在明显差异，在草甘膦剂量为0.92 kg·hm-2时，石豆1号存活率为100%，表现了较高的抗性，8份材料的存活率为4%～48%，表现出一定的抗性；44份材料表现敏感，存活率为0。试验研究了抗性材料石豆1号、中抗材料Williams和敏感材料中黄35对草甘膦的生理生化反应的差异，结果表明：随着草甘膦处理剂量的增加和处理时间的延长，石豆1号莽草酸含量、叶绿素含量和超氧化物歧化酶（SOD）相对活性没有明显变化。而Williams和中黄35的莽草酸含量和SOD相对活性明显升高，叶绿素含量明显降低。结果表明草甘膦（浓度0.92 kg·hm-2）处理下，不同抗性栽培大豆叶片中莽草酸含量有明显差异，处理5～14 d时敏感材料叶片中莽草酸含量保持较高水平，因此栽培大豆叶片莽草酸含量可以作为判断其对草甘膦抗性高低的主要生理指标之一，而SOD的活性和叶绿素含量可以作为判断栽培大豆对草甘膦抗性的辅助生理指标。
Abstract:
In this paper, the resistance of different soybean cultivars to glyphosate was studied systematically by field experiment combined with biochemtcal analysis. The results showed that under the effective glyphosate dose of 0.31-0.92 kg·ha-1, there was a significant difference in glyphosate resistance among different soybean cultivars. When glyphosate dose was 0.92 kg·ha-1, the survival rate of Shidou 1 was 100%, showed high resistance, the survival rate of the other 8 materials was 4%-48%, showed certain resistance, 44 materials were sensitive, and the survival rate was 0. The physiological and biochemical reaction of different resistant soybean materials were as follows, with the increase of glyphosate treatment dose and the extension of treatment time, shikimic acid chlorophyll content and SOD activity of Shidou 1 didn’t change significantly. It showed that Shidou 1 had stronger resistance to glyphosate, while the shikimic acid and SOD of the other two kinds soybean materials increased significantly. This result indicated that the accumulation of shikimic acid in soybean can be used as a judgment of one of the main physiological indexes of resistance to glyphosate.

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备注/Memo

基金项目：转基因生物新品种培育重大专项（2016ZX08004001）。

第一作者简介：商璐（1990-），男，硕士，主要从事抗草甘膦大豆种质挖掘及抗性机制研究。E-mail：330662161@qq.com。
通讯作者：陶波（1963-），男，教授，主要从事抗除草剂作物基因的挖掘研究。E-mail：botaol@163.com。

更新日期/Last Update: 2017-03-14