[1]李冬梅,张可欣,李文滨.大豆GmMP基因的功能预测及表达分析[J].大豆科学,2017,36(01):28-32.[doi:10.11861/j.issn.1000-9841.2017.01.0028]
　LI Dong-mei,ZHANG Ke-xin,LI Win-bin.Functional Prediction and Expression Analysis of GmMP in Soybean[J].Soybean Science,2017,36(01):28-32.[doi:10.11861/j.issn.1000-9841.2017.01.0028]
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大豆GmMP基因的功能预测及表达分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年01期 页码: 28-32 栏目: 出版日期: 2017-01-20
Title:
Functional Prediction and Expression Analysis of GmMP in Soybean
作者:
李冬梅; 张可欣; 李文滨
（东北农业大学 大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室，黑龙江 哈尔滨 150030）
Author(s):
LI Dong-mei;  ZHANG Ke-xin; LI Win-bin
Key Laboratory of Soybean Biology in Chinese Ministry of Education / Key Laboratory of Northeast Soybean Biology and Genetic Breeding Agriculture Ministry / Northeast Agricultural University, Harbin 150030, China
关键词:
大豆;  GmMP基因;  生长素;  生物信息学
Keywords:
Soybean;  GmMP gene;  Auxin;  Bioinformatics
DOI:
10.11861/j.issn.1000-9841.2017.01.0028
文献标志码:
A
摘要:
通过在大豆基因组数据库中检索拟南芥AtMP(ARF5)在大豆中的同源基因，获得了GmMP基因序列。对GmMP基因编码的氨基酸序列及启动子序列进行生物信息学分析，结果表明：GmMP基因CDS序列全长2 802 bp, 编码933个氨基酸。GmMP编码的蛋白为疏水性蛋白。结构域分析表明：GmMP含有B3和AUXIN RESPONSE FACTOR结构域，同时该基因是ARF家族的成员。GmMP预测的启动子区域含有与激素、胁迫、光应答、生物钟调控和转录因子结合相关的顺式作用元件。系统进化分析表明MP在豆科植物进化过程中比较保守。组织特异性表达分析结果显示GmMP在叶片中表达量最低，在茎尖中表达量最高，推测其可能参与生长素的代谢途径。
Abstract:
Abstract:GmMP gene sequence was obtained by searching the homologous gene of AtMP in the soybean genome database. Bioinformaties analysis of amino acid sequence encoded by GmMP and promoter sequences showed that the full-length CDS sequence was 2 802 bp, encoding 933 amino acids protein. Structure domains analysis indicated that GmMP contained B3 and AUXIN RESPONSE FACTOR domain, belong to B3 and AUXIN RESPONSE superfamily. Cis-elements associated with hormones, stresses, light responses, circadian rhythms and transcription factors binding were existed in the predicted promoter region. Phylogenetic analysis results suggested that MP was conserved in the leguminous plants. Results of tissue specific analysis revealed that expression level of GmMP was the lowest in leaves, the highest in SAM, it was speculated to participate in the auxin metabolic pathways.

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备注/Memo

基金项目：抗逆转基因大豆新品种培育专项(2016ZX08004-002)。

第一作者简介：李冬梅（1980-），女，硕士，实验师，主要从事大豆遗传转化、转基因检测等方面研究。E-mail：yy841026@163.com。
通讯作者： 李文滨（1958-），男，教授，博导，主要从事大豆生物技术研究。E-mail：wenbinli@yahoo.com。

更新日期/Last Update: 2017-03-14