[1]翟莹,张军,赵艳,等.大豆SbPRP1和SbPRP2基因在非生物胁迫下的表达及载体构建[J].大豆科学,2017,36(01):24-27.[doi:10.11861/j.issn.1000-9841.2017.01.0024]
　ZHAI Ying,ZHANG Jun,ZHAO Yan,et al.Expression and Vector Construction of SbPRP1 and SbPRP2 in Soybean Under Abiotic Stresses[J].Soybean Science,2017,36(01):24-27.[doi:10.11861/j.issn.1000-9841.2017.01.0024]
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大豆SbPRP1和SbPRP2基因在非生物胁迫下的表达及载体构建

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年01期 页码: 24-27 栏目: 出版日期: 2017-01-20
Title:
Expression and Vector Construction of SbPRP1 and SbPRP2 in Soybean Under Abiotic Stresses
作者:
翟莹1; 张军2; 赵艳1; 任巍巍1; 张闯1
1.齐齐哈尔大学 生命科学与农林学院，黑龙江 齐齐哈尔 161006； 2.黑龙江省兽医科学研究所，黑龙江 齐齐哈尔 161005
Author(s):
ZHAI Ying1; ZHANG Jun2; ZHAO Yan1; REN Wei-wei1; ZHANG Chuang1
(1.College of Life Science and Agro-Forestry, Qiqihar University, Qiqihar 161006, China; 2.Heilongjiang Institute of Veterinary Science, Qiqihar 161005, China)
关键词:
大豆; 脯氨酸富集蛋白; 表达分析; 载体构建; 抗逆性
Keywords:
Soybean;  Prolinerich protein;  Expression analysis;  Vector construction;  Stress resistance
DOI:
10.11861/j.issn.1000-9841.2017.01.0024
文献标志码:
A
摘要:
为研究大豆细胞壁脯氨酸富集蛋白基因在非生物胁迫下发挥的作用，利用实时荧光定量PCR，对大豆中的两个脯氨酸富集蛋白基因SbPRP1和SbPRP2在非生物胁迫下的表达情况进行检测。结果显示：SbPRP1在高盐和低温处理下表达量下降，分别在处理后1 和24 h达到最低值；在模拟干旱处理下表达量先下降后升高，处理后24 h达到最大值。SbPRP2在高盐、模拟干旱和低温处理下表达量均有所升高，分别在处理后24，1 和5 h达到最大值。表明SbPRP1和SbPRP2可能参与了大豆对不利环境的适应性调控。通过RT-PCR从大豆叶片中扩增SbPRP1和SbPRP2的基因全序列并构建到植物表达载体pRI101-AN上。
Abstract:
Plant proline-rich proteins (PRPs) are putative cell wall proteins, which are usually associated with abiotic stress. The expression of SbPRP1 and SbPRP2 under abiotic stress treatments in soybean was detected by real-time fluorescence quantitative PCR. The expression of SbPRP1 was decreased under salt and cold treatments, reaching the minimum expression at 1 and 24 h, respectively. While it increased firstly and thereafter declined under simulated drought treatment, reaching the maximum expression at 24 h. The expression of SbPRP2 was increased under salt, simulated drought and cold treatments, reaching the maximum expression at 24, 1 and 5 h, respectively. These results indicate that SbPRP1 and SbPRP2 may involve in the regulation of adapting to adverse environment in soybean. The SbPRP1 and SbPRP2 were amplified through RT-PCR from soybean leaves and constructed to plant expression vector of pRI101-AN.

参考文献/References:

［1］蒋炳军, 岳岩磊, 王彩洁, 等. 大豆分子育种研究进展［J］. 大豆科学, 2012, 31(4): 662-667. (Jiang B J, Yue Y L, Wang C J, et al. Recent advances in molecular breeding of soybean［J］. Soybean Science, 2012, 31(4): 662-667.)

［2］韩青, 陈瑞, 杨野, 等. 植物富含脯氨酸蛋白的研究进展［J］. 植物生理学报, 2015, 51(8): 1179-1184. (Han Q, Chen R, Yang Y, et al. Progress on plant proline-rich protein［J］. Plant Physiology Journal, 2015, 51 (8): 1179-1184.)
［3］翟莹, 张军, 陈阳, 等. 大豆GmPRP转基因拟南芥抗盐性鉴定［J］. 大豆科学, 2015, 34(2): 345-348. (Zhai Y, Zhang J, Chen Y, et al. Salt tolerance identification of soybean GmPRP- transgenic Arabidopsis［J］. Soybean Science, 2015, 34(2): 345-348.)
［4］Lawton M, Lamb C. Transcriptional activation of plant defense genes by fungal elicitor, wounding and infection［J］. Molecular and Cellular Biology, 1987, 7(1): 335-341.
［5］Showalter A, Butt A, Kim S. Molecular details of tomato extensin and glycine-rich protein gene expression［J］. Plant Molecular Biology, 1992, 19(2): 205-215.
［6］Didierjean L, Frendo P, Burkard G. Stress responses in maize: Sequence analysis of cDNAs encoding glycine-rich proteins［J］. Plant Molecular Biology, 1992, 18(4): 847-849.
［7］Didierjean L, Frendo P, Nasser W, et al. Heavy metal-responsive genes in maize: Identification and comparison of their expression upon various forms of abiotic stress［J］. Planta, 1996, 199(1): 1-8.
［8］Datta K, Schmidt A, Marcus A. Characterization of two soybean repetitive proline-rich proteins and a cognate cDNA from germinated axes［J］. The Plant Cell, 1989, 1: 945-952.?
［9］Kleis S, Tierney M. Isolation and characterization of a proline-rich cell wall protein from soybean seedling［J］. Plant Physiology, 1990, 94: 1897-1902.
［10］Bradley D, Kjellbom P, Lamb C. Elicitor-and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: A novel，rapid defense response［J］. Cell, 1992, 70(1): 21-30.
［11］Brisson L, Tenhaken R, Lamb C. Function of oxidative cross-Linking of cell wall structural proteins in plant disease resistance［J］. The Plant Cell, 1994, 6(12): 1703-1712.
［12］Priyanka B, Sekhar K, Sunita T, et al. Characterization of expressed sequence tags (ESTs) of pigeonpea (Cajanus cajan L.) and functional validation of selected genes for abiotic stress tolerance in Arabidopsis thaliana［J］. Molecular Genetics and Genomics, 2010, 283(3): 273-287.
［13］Gothandam K, Nalini E, Karthikeyan S, et al. OsPRP3, a flower specific proline-rich protein of rice, determines extracellular matrix structure of floral organs and its overexpression confers cold-tolerance［J］. Plant Molecular Biology, 2010, 72(1): 125-135.
［14］Zhang Y, Schl-ppi M. Cold responsive EARLI1 type HyPRPs improve freezing survival of yeast cells and form higher order complexes in plants［J］. Planta, 2007, 227(1): 233-243.
［15］Zhan X, Wang B, Li H, et al. Arabidopsis proline-rich protein important for development and abiotic stress tolerance is involved in microRNA biogenesis［J］. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(44): 18198-18203.
［16］吕高强, 吴向阳, 王心宇. 芝麻中一个富含脯氨酸新基因的克隆与特征分析［J］. 作物学报, 2015, 41(12): 1810-1818. (Lyu G Q, Wu X Y, Wang X Y. Cloning and characterization of a novel gene encoding proline-rich protein in sesame［J］. Acta Agronomica Sinica, 2015, 41(12): 1810-1818.)
［17］Hong J, Nagao R, Key J. Characterization and sequence analysis of a developmentally regulated putative cell wall protein gene isolated from soybean［J］. The Journal of Biological Chemistry, 1987, 262 (17): 8367-8376.
［18］Hong J, Nagao R, Key J. Characterization of a proline-rich cell wall protein gene family of soybean［J］. The Journal of Biological Chemistry, 1990, 265(5): 2470-2475.?
［19］Hong J, Nagao R, Key J. Developmentally regulated expression of soybean proline-rich cell wall protein genes［J］. The Plant Cell, 1989, 1: 937-943.?
［20］Wyatt R, Nagao R, Key J. Patterns of soybean prolinerich protein gene expression［J］. The Plant Cell, 1992, 4: 99-110.
［21］柴团耀, 张玉秀. 菜豆富含脯氨酸蛋白质基因在生物和非生物胁迫下的表达［J］. 植物学报, 1999, 41(l): 111-113. (Chai T Y, Zhang Y X. Gene expression analysis of a proline-rich protein from bean under biotic and abiotic stress［J］. Acta Botanica Sinica, 1999, 41(l): 111-113.)
［22］张德静, 秦丽霞, 李龙, 等. 异源表达棉花-GhPRP5-基因增强了拟南芥对盐和ABA的敏感性［J］. 作物学报, 2013, 39(3): 563-569. (Zhang D J, Qin L X, Li L, et al. Expression of cotton GhPRP5 gene in Arabidopsis enhances susceptibility to ABA and salt stresses［J］. Acta Agronomica Sinica, 2013, 39(3): 563-569.)
［23］Sheng J, Dovidio R, Mehdy M. Negative and positive regulation of a novel proline-rich protein mRNA by fungal elicitor and wounding［J］. The Plant Journal, 1991, 1(3): 345-354.
［24］杨君, 张艳, 王伟巧, 等. 海岛棉GbHyPRP1克隆及其转基因拟南芥抗黄萎病验证［J］. 植物遗传资源学报, 2015, 16(3): 594-602. (Yang J, Zhang Y, Wang W Q, et al. Cloning of GbHyPRP1 from Gossypium barbadense and validation of Verticillium wilt resistance in transgenic Arabidopsis［J］. Journal of Plant Genetic Resources, 2015, 16(3): 594-602.)
［25］Showalter A, Keppler B, Lichtenberg J, et al. A bioinformatics approach to the identification, classification, and analysis of hydroxyproline-rich glycoproteins［J］. Plant Physiology, 2010, 153(2): 485-513.
［26］Goodwin W, Pallas J, Jenkins G. Transcripts of a gene encoding a putative cell wall-plasma membrane linker protein are specifically cold-induced in Brassica napus［J］. Plant Molecular Biology, 1996, 31(4): 771-781.
［27］He C, Zhang J, Chen S. A soybean gene encoding a proline-rich protein is regulated by salicylic acid, an endogenous circadian rhythm and by various stresses［J］. Theoretical and Applied Genetics, 2002, 104(6-7): 1125-1131.

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备注/Memo

基金项目：国家自然科学基金（31301335）；黑龙江省教育厅科学技术研究(12541889)；黑龙省自然科学基金项目（C201458）；黑龙江省普通本科高等学校青年创新人才培养计划（UNPYSCT_2016090）。

第一作者简介：翟莹（1982-），女，博士，副教授，主要从事大豆分子育种研究。E-mail：fairy39809079@126.com。

更新日期/Last Update: 2017-03-14