[1]宁文峰,庞添,杨艳玲,等.大豆GmWRKY20基因表达特性研究[J].大豆科学,2016,35(05):748-753.[doi:10.11861/j.issn.1000-9841.2016.05.0748]
　NING Wen-feng,PANG Tian,YANG Yan-ling,et al.Expression Analysis of GmWRKY20 in Soybean[J].Soybean Science,2016,35(05):748-753.[doi:10.11861/j.issn.1000-9841.2016.05.0748]
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大豆GmWRKY20基因表达特性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年05期 页码: 748-753 栏目: 出版日期: 2016-09-20
Title:
Expression Analysis of GmWRKY20 in Soybean
作者:
宁文峰1; 庞添1; 杨艳玲1; 张超1; 2; 柏锡1
（1.东北农业大学 生命科学学院，黑龙江 哈尔滨 150030； 2.齐齐哈尔市园艺研究所，黑龙江 齐齐哈尔 161006）
Author(s):
NING Wen-feng1;  PANG Tian1;  YANG Yan-ling1;  ZHANG Chao1; 2;  BAI Xi1
College of Life Science, Northeast Agricultural University, Harbin 150030, China; 2.Qiqihar Horticultural Research Institute, Qiqihar 161006, China
关键词:
大豆; GmWRKY20基因; 表达分析; 非生物胁迫
Keywords:
soybean;  GmWRKY20 gene;  Expression analysis;  Abiotic stress
DOI:
10.11861/j.issn.1000-9841.2016.05.0748
文献标志码:
A
摘要:
GsWRKY20基因是从野生大豆中挖掘到的非生物胁迫相关基因，该基因可以提高转基因拟南芥与苜蓿的抗旱性，并使拟南芥提前开花。栽培大豆中的GmWRKY20是GsWRKY20基因的同源基因，序列相似度达到99%。利用实时荧光定量PCR分析栽培大豆中GmWRKY20基因表达特性，结果表明：GmWRKY20基因在不同栽培品种间的表达没有显著差异；仅在大豆发育的特定阶段上调表达，其上调表达与大豆开花的起始相关；主要表达部位在叶片；不受光周期影响，但受到多种非生物胁迫影响。
Abstract:
GsWRKY20 was identified from Glycine soja as an abiotic stress related gene in our previous studies. Overexpression of GsWRKY20 could enhance the drought tolerance of transgenic Arabidopsis or Alfalfa, and promote the flowering of transgenic Arabidopsis. GsWRKY20 encodes a WRKY.type transcription factor, with 99% identity to GmWRKY20 from Glycine max. In order to fully investigate the role and the possible molecular mechanism of GmWRKY20 in soybean, the expression of GmWRKY20 in cultivated soybean was fully studied by real-time RT-PCR. The results showed that the expression of GmWRKY20 did not show obvious differences among different soybean cultivars, and high expression of GmWRKY20 was observed before flowering, suggesting that the high expression of GmWRKY20 might play an important role in starting flowering. GmWRKY20 did not response to photoperiod but response to multiple abiotic stresses at transcript level.

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备注/Memo

基金项目：国家自然科学基金（31371544）；转基因生物新品种培育重大专项（2016ZX08004-002）。第一作者简介：宁文峰（1989-），男，硕士，主要从事植物学研究。E-mail：ningwenfeng1231@126.com。通讯作者：柏锡（1975-），男，副教授，主要从事植物分子生物学和逆境生理学研究。E-mail：baixi@neau.edu.cn。

更新日期/Last Update: 2016-09-25