[1]接伟光,于文杰,蔡柏岩.摩西管柄囊霉与连作大豆根腐病原菌尖孢镰刀菌的相互关系研究[J].大豆科学,2016,35(04):637-642.[doi:10.11861/j.issn.1000-9841.2016.04.0637]
　JIE Wei-guang,YU Wen-jie,CAI Bai-yan.Ｒesearch on the Ｒelationship Between Funneliformis mosseae and the Ｒoot ＲotPathogen Fusarium Oxysporum in the Continuous Cropping of Soybean[J].Soybean Science,2016,35(04):637-642.[doi:10.11861/j.issn.1000-9841.2016.04.0637]
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摩西管柄囊霉与连作大豆根腐病原菌尖孢镰刀菌的相互关系研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年04期 页码: 637-642 栏目: 出版日期: 2016-07-20
Title:
Ｒesearch on the Ｒelationship Between Funneliformis mosseae and the Ｒoot ＲotPathogen Fusarium Oxysporum in the Continuous Cropping of Soybean
作者:
接伟光1; 于文杰2; 蔡柏岩2
1. 黑龙江东方学院食品与环境工程学部，黑龙江哈尔滨150066; 2. 黑龙江大学生命科学学院/黑龙江省普通高等学校微生物重点实验室，黑龙江哈尔滨150080
Author(s):
JIE Wei-guang1; YU Wen-jie2; CAI Bai-yan2
( 1. Department of Food and Environment Engineering，East University of Heilongjiang，Harbin 150086，China; 2. Key Laboratory of Microbiology，Collegeof Life Sciences，Heilongjiang University，Harbin 150080，China)
关键词:
大豆;  摩西管柄囊霉;  尖孢镰刀菌;  连作
Keywords:
Soybean;  Funneliformis mosseae;  Fusarium oxysporum;  Continuous cropping
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.04.0637
文献标志码:
A
摘要:
以黑农44( 高脂肪品种) 大豆品种作为试验材料，以不同方式在连作大豆根际土壤中接种摩西管柄囊霉( Funneliformismosseae) 和大豆根腐病原菌尖孢镰刀菌( Fusarium oxysporum) 。采用传统形态学方法检测Fu. mosseae 和F. oxysporum侵染大豆植株根系情况。利用实时荧光定量PCＲ 技术分析Fu. mosseae 和F. oxysporum DNA 含量变化。结果表明: Fu. mosseae 和F. oxysporum 均能侵染大豆植株根系，但接种Fu. mosseae 的大豆植株根系及根际土壤样品中F. oxysporum DNA 含量显著降低，表明Fu. mosseae 对F. oxysporum 具有强烈的抑制作用。本研究为进一步研究AM 真菌克服大豆连作障碍提供了理论依据。
Abstract:
In this study，Heinong 44 ( a high-fat cultivar) soybean cultivar was selected as materials. Funneliformis mosseaeand the root rot pathogen Fusarium oxysporum were inoculated into the soybean continuous cropping soil in different treatments.The colonisation rates of Fu. mosseae and disease incidences of F. oxysporum were analyzed statistically，respectively.Quantitative PCＲ was applied to analyze the DNA content of Fu. mosseae and F. oxysporum. The results showed that both Fu.mosseae and F. oxysporum could infect the soybean roots. However，the DNA content of F. oxysporum clearly decreased insoybean root and rhizosphere soil samples after the inoculation with Fu. mosseae. It might indicate inhibitive effects of Fu.mosseae over F. oxysporum. It provides theoretical evidence on the AM fungi to overcome the obstacles of soybean continuouscropping.

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备注/Memo

基金项目: 黑龙江省普通高等学校青年学术骨干支持计划( 1254G049) 。第一作者简介: 接伟光( 1981-) ，男，博士，副教授，主要从事微生物生态学研究。E-mail: jieweiguang2007@126． com。通讯作者: 蔡柏岩( 1968-) ，男，博士，教授，主要从事生态学研究。E-mail: caibaiyan@126． com。

更新日期/Last Update: 2016-08-23