[1]刘宇原,付爱根,徐敏.大豆转座子的研究现状及应用前景[J].大豆科学,2016,35(03):512-518.[doi:10.11861/j.issn.1000-9841.2016.03.0512]
　LIU Yu-yuan,FU Ai-gen,XU Min.Ｒesearch Status and Application Prospect of Soybean Transposon[J].Soybean Science,2016,35(03):512-518.[doi:10.11861/j.issn.1000-9841.2016.03.0512]
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大豆转座子的研究现状及应用前景

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年03期 页码: 512-518 栏目: 出版日期: 2016-05-20
Title:
Ｒesearch Status and Application Prospect of Soybean Transposon
作者:
刘宇原; 付爱根; 徐敏
西北大学生命科学学院，陕西西安710069
Author(s):
LIU Yu-yuan; FU Ai-gen; XU Min
College of Life Science，Northwest University，Xi’an 710069，China
关键词:
大豆;  转座子;  突变体库;  功能基因组学
Keywords:
Soybean;  Transposon;  Mutant library;  Functional genomics
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.03.0512
文献标志码:
A
摘要:
大豆基因组中约有5 万基因，大部分基因的功能处于未知状态。转座子插入突变体库的应用可以为解码大豆基因组中未知基因的功能提供研究平台，同时，也可以为创建有育种应用价值的大豆突变体种质资源库提供基础。大豆基因组中50% ～ 60%的组分为转座子，其中具有活性的且结构完整的转座子只有Tgm9。大豆转座子突变体库目前主要还是利用外源转座子来建立，但是开发并改进内源转座子，增加其跳跃能力及稳定性，可以成为构建突变体库的一个新方向。本文主要介绍了大豆基因组中发现的各种转座子及其特点，并且重点讨论了外源转座子和大豆内源转座子在大豆突变体库创建中的应用现状及应用前景。
Abstract:
There are about 50 000 genes in the soybean genome，and the function of majority genes remains to be determined.A comprehensive insertion mutant library could provide a platform for the functional characterization of unknown genes in soybeangenome，and it could also contribute a collection of soybean germplasm resources with a variety of breeding values.Transposon works as a good tool for creating insertion mutants. In soybean，approximately 50%-60% of the genome are transposons，including retrotransposons and DNA transposons，while except DNA transposable elements Tgm9，all the elements areinactive. Many mutants were verified through transposon tagging experiment using T322，a soybean line containing activeTgm9. Although several mutant libraries were made using transposons from other species through gene transformation，developingnative elements and improving their activity and consistence shall provide a new method in soybean mutagenesis. In thispaper，we introduced transposons identified in soybean and the recent progress on applications of transposons in creating soybeaninsertion mutants for soybean functional genomics studies and germplasm renovation.

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备注/Memo

基金项目: 陕西省教育厅重点实验室科研计划项目( 13JS108) ; 陕西省科技统筹创新工程项目( 2014KTCL02-03) 。

第一作者简介: 刘宇原( 1990-) ，女，硕士，主要从事大豆转座子的改造与利用。E-mail: 15929802100@163. com。
通讯作者: 徐敏( 1973-) ，女，博士，副教授，主要从事大豆分子遗传研究。E-mail: xumin@ nwu. edu. cn。

更新日期/Last Update: 2016-07-24