[1]薛忠财,高辉远.反射光谱技术在监测大豆叶片光化学效率和水分状况中的应用[J].大豆科学,2016,35(03):436-441.[doi:10.11861/j.issn.1000-9841.2016.03.0436]
　XUE Zhong-cai,GAO Hui-yuan.Application of Spectral Ｒeflectance Technique on Monitoring the PhotochemicalEfficiency and Water Condition in Soybean Leaves[J].Soybean Science,2016,35(03):436-441.[doi:10.11861/j.issn.1000-9841.2016.03.0436]
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反射光谱技术在监测大豆叶片光化学效率和水分状况中的应用

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年03期 页码: 436-441 栏目: 出版日期: 2016-05-20
Title:
Application of Spectral Ｒeflectance Technique on Monitoring the PhotochemicalEfficiency and Water Condition in Soybean Leaves
作者:
薛忠财1; 2; 3; 高辉远1
1. 山东农业大学生命科学学院/作物生物学国家重点实验室，山东泰安271000;
2. 山东农业大学林学院，山东泰安271000;?
3. 清华大学核能与新能源技术研究院，北京100084
Author(s):
XUE Zhong-cai1; 2; 3; GAO Hui-yuan1
1. College of Life Science，Shandong Agricultural University /State Key Laboratory of Crop Biology，Tai’an 271018，China;?
2. Forestry School，ShandongAgricultural University，Tai’an 271018，China;?
3. Institute of Nuclear and New Energy Technology，Tsinghua University，Beijing 100084，China
关键词:
大豆;  光化学反射指数;  反射光谱;  水分指数;  失水;  叶绿素荧光
Keywords:
Soybean;  Photochemical reflectance index;  Spectral reflectance;  Water index;  Dehydration;  Chlorophyll fluorescence
DOI:
10.11861/j.issn.1000-9841.2016.03.0436
文献标志码:
A
摘要:
干旱胁迫在我国大豆主产区频繁发生，对大豆产量造成了严重影响。因此，为建立有效的旱情监测指标，采用反射光谱技术监测了野生大豆和栽培大豆离体叶片在失水过程中相对含水量和叶绿素荧光的变化。结果表明: 在失水过程中，野生大豆和栽培大豆离体叶片的相对含水量( ＲWC) 、水分指数( WI) 、光化学反射指数( PＲI) 、最大光化学效率( Fv /Fm) 和实际光化学效率( ФPSII) 都随失水时间的增加而降低，且WI、PＲI、Fv /Fm 和ФPSII均与ＲWC 呈显著相关，PＲI 可以很好地反映Fv /Fm 和ФPSII的变化。同时野生大豆和栽培大豆离体叶片在可见光波段( 500 ～ 750 nm)的反射率随着失水时间的增加而升高，在近红外区( 750 ～ 1 000 nm) 的反射率则呈现“升高－ 降低－ 升高”的趋势。总之，利用反射光谱技术可以快速、准确地反映大豆叶片在失水过程中光化学效率和水分状况的变化。
Abstract:
Drought，which occured frequently in the major soybean producing areas in our country，results in a serious reductionin soybean yield. To establish effectively drought monitoring indicators，we monitored the changes of relative water contentsand chlorophyll fluorescence in the detached leaves of wild and cultivated soybean species during dehydration by applyingspectral reflectance technique. The results showed that the relative water contents ( ＲWC) ，the water index ( WI) ，the photochemicalreflectance index ( PＲI) ，the maximum PSII quantum yield ( Fv /Fm) and the actual photochemical efficiency ofPSII ( ФPSII) in the detached leaves of both wild and cultivated soybean plants decreased with the increasing of dehydrationtime. And the ＲWC was significantly correlated with the WI，PＲI，Fv /Fm and ФPSII. In addition，the PＲI was well correlatedwith Fv /Fm and ФPSII. The leaf reflectance increased in visual range ( 500-670 nm) with the increasing of the dehydrationtime of both wild and cultivated soybean leaves，whereas the changes in near-infrared range ( 750 -1 000 nm) followed an‘increase-decrease-increase’trend. In conclusion，the changes of spectral reflectance were convenient and precise indicatorto reflect light use efficiency and water condition in the leaves of soybean during dehydration.

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备注/Memo

基金项目: 国家自然科学基金( 31370276) 。第一作者简介: 薛忠财( 1985-) ，男，博士，主要从事环境生态学研究。E-mail: zhongcaix2008@163. com。通讯作者: 高辉远( 1958-) ，男，博士，教授，主要从事生理生态学研究。E-mail: gaohy@ sdau. edu. cn。

更新日期/Last Update: 2016-06-28