[1]杨晓贺,顾鑫,于铭,等.三江平原主栽大豆品种对大豆疫霉根腐病的抗性分析[J].大豆科学,2016,35(02):291-294.[doi:10.11861/j.issn.1000-9841.2016.02.0291]
　YANG Xiao-he,GU Xin,YU Ming,et al.Resistance of Main Cultivars Against Phytophthora sojae Causing Soybean Root Rot in Sanjiang Plain Area[J].Soybean Science,2016,35(02):291-294.[doi:10.11861/j.issn.1000-9841.2016.02.0291]
点击复制

三江平原主栽大豆品种对大豆疫霉根腐病的抗性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年02期 页码: 291-294 栏目: 出版日期: 2016-03-20
Title:
Resistance of Main Cultivars Against Phytophthora sojae Causing Soybean Root Rot in Sanjiang Plain Area
作者:
杨晓贺1; 顾鑫1; 于铭2; 赵海红1; 姚亮亮1; 刘伟1; 申宏波3; 丁俊杰1
1.黑龙江省农业科学院 佳木斯分院/农业部佳木斯作物有害生物科学观测试验站，黑龙江 佳木斯 154007；
?2.黑龙江省绿色食品发展中心，黑龙江 哈尔滨 150090；
?3.黑龙江省农业职业技术学院，黑龙江 佳木斯 154007
Author(s):
YANG Xiao-he1;  GU Xin1;  YU Ming2;  ZHAO Hai-hong1;  YAO Liang-liang1;  LIU Wei1;  SHEN Hong-bo3;  DING Jun-jie1
1. Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences / Observation and Experiment Station of Crop Pests of Jiamusi, Ministry of Agriculture, Jiamusi 154007, China;?
2. Green Food Development Center of Heilongjiang Province, Harbin 150090, China;?
3. Heilongjiang Agricultural College of Vocational Technology, Jiamusi 154007, China.
关键词:
大豆根腐病; 大豆疫霉菌; 人工接种
Keywords:
Soybean root rot;  Phytophthora sojae;  Artificial inoculation
DOI:
10.11861/j.issn.1000-9841.2016.02.0291
文献标志码:
A
摘要:
为了明确三江平原地区主栽大豆品种对大豆疫霉根腐病的抗性，对合丰56、合丰55、合丰50等13个主栽品种进行了大豆疫霉菌1号小种的盆栽接种和大田接种抗性分析试验。结果表明：合丰55在盆栽接种鉴定中表现为抗病，死亡率为27.41%，与其它品种相比，除了垦丰16和绥农28外，差异极显著。其在大田接种鉴定中表现为抗病，死亡率为3.33%，与其它品种相比，除垦丰16外，差异显著；大田接种减产率为3.84%，与未接种对照相比，差异显著。垦丰16在盆栽接种鉴定中表现为抗病，死亡率为24.81%，与其它品种相比，除了合丰55和绥农28外，差异极显著；在大田接种鉴定中表现为抗病，死亡率为6.67%，与其它品种相比，除合丰55外，差异显著；大田接种减产率为5.19%，与未接种对照相比，差异显著。研究结果表明，合丰55和垦丰16具有良好的抗性，大田接种试验表现减产率低，是适合三江平原地区种植的抗大豆疫霉根腐病的优良品种。
Abstract:
In order to confirm the resistance of main cultivars against.Phytophthora sojae causing soybean root rot in Sanjiang plain area, identification of 13 main cultivars in Sanjiang plain to race 1 of Phytophthora sojae with pot and field test by artificial inoculation was done.The results showed that the resistance of Hefeng 55 was resistant in pot test with mortality for 27.41%, and the difference was extremely significant compared to other cultivars except for Kenfeng 16 and Suinong 28.The resistance of Hefeng 55 was resistant in field test with mortality for 3.33%, and the difference was significant compared to other cultivars except for Kenfeng 16. Decreasing rate of Hefeng 55 was 3.84% compared to the control check without inoculation, and the difference was significant. The resistance of Kenfeng 16 was resistant in pot test with mortality for 24.81%, and the difference was extremely significant compared to other cultivars except for Hefeng 55 and Suinong 28. The resistance of Kenfeng 16 was resistant in field with mortality for 6.67%, and the difference was significant compared to other cultivars except for Hefeng 55. Decreasing rate of Kenfeng 16 was 5.19% compared to the control check without inoculation, and the difference was significant. The results showed that Hefeng 55 and Kenfeng 16 were suitable to plant to control the soybean root rot causing by Phytophthora sojae in Sanjiang plain area because of their resistance were better and decreasing rates were lower.

参考文献/References:

［1］Schmitthenner A F. Problems and progress in control of Phytophthora-root rot of soybean［J］. Plant Disease, 1985, 69 (4): 362-368

［2］吕慧颖, 孔凡江, 许修宏, 等.东北三省大豆种质资源对大豆疫霉根腐病的抗性表现［J］. 中国油料作物学报, 2000, 23(4): 16-18.(Lyu H Y, Kong F J, Xu X H, et al.Preliminary studies on biological characteristics of Phytophthora Megasperma var sojiae［J］.Chinese Journal of Oil Crop Sciences, 2000, 23(4): 16-18.)
［3］沈崇尧, 苏彦纯. 中国大豆疫霉病菌的发现及初步研究［J］. 植物病理学报, 1991, 21(4): 298.(Shen C Y, Su Y C. Discovery and preliminary studies of Phytophthora megasperma on soybean in China［J］.Acta Phytopathologica Sinica,1991, 21(4): 298.)
［4］许修宏, 吕慧颖, 杨庆凯, 等.大豆疫霉根腐病抗源筛选［J］.大豆科学, 1999, 18(2): 147-150.(Xu X H, Lyu H Y, Yang Q K, et al.Screening of soybean germplasms for resistance to Photophthora sojae［J］. Soybean Science, 1999, 18(2): 147-150.)
［5］朱振东, 王晓鸣, 王化波, 等.蒙城大豆疫霉菌的鉴定及其生理小种［J］.植物病理学报, 2001, 31(3): 236-240.(Zhu Z D, Wang X M, Wang H B, et al. Identification and race of Phytophthora sojae isolates collected in MengCheng, Anhui province［J］. Acta Phytopathologica Sinica, 2001, 31(3): 236-240.)
［6］朱振东, 王化波, 王晓鸣, 等.中国大豆疫霉菌分布及毒力多样性研究［J］.中国农业科学, 2003, 36(7): 793-799.(Zhu Z D, Wang H B, Wang X M, et al. Distribution and virulence diversity of Phytophthora sojae in China［J］. Scientia Agricultura Sinica, 2003, 36(7): 793-799.)
［7］李宝英, 马淑梅.大豆根腐病病原菌种类及抗原筛选［J］.植物保护学报, 2000, 27(1): 91-92. (Li B Y, Ma S M. Pathogens of soybean root rot and screening of resistant sources［J］, Acta Phytophylacica Sinica, 2000, 27(1):91-92.)
［8］霍云龙, 朱振东, 李向华, 等. 抗大豆疫霉根腐病野生大豆资源的初步筛选［J］. 植物遗传资源学报, 2005, 6(2): 182-185.(Huo Y L, Zhu Z D, Li X H, et al. Preliminary screening for Phytophthora root rot resistance in wild soybean［J］. Journal Plant Genetic Resoures, 2005, 6(2): 182-185.)
［9］朱振东, 王晓鸣, 常汝镇, 等.黑龙江省大豆疫霉生理小种鉴定及大豆种质的抗性评价［J］. 中国农业科学, 2000, 33(1): 62-67.(Zhu Z D, Wang X M, Chang R Z, et al.Identification of race of Phytophthora sojae and reaction of soybean germplasm resoures in Heilongjiang province［J］. Scientia Agricultura Sinica, 2000, 33(1): 62-67.)
［10］马淑梅, 李宝英, 丁俊杰. 大豆疫霉根腐病抗病资源筛选及抗性遗传研究［J］. 大豆科学, 2001,20(3): 197-199.(Ma S M, Li B Y, Ding J J. Selections of soybean germplasms with resistance to Phytophthora- root rot and its use in rteeding for resistance［J］. Soybean Science, 2001, 20(3): 197-199.)
［11］顾鑫, 丁俊杰.大豆对主要病害多抗性种质资源鉴定［J］. 中国农学通报, 2010, 26(14): 256-260.(Gu X, Ding J J. The multi-resistant identification of soybean to main diseases［J］. Chinese Agricultural Science Bulletin, 2010, 26(14): 256-260.)
［12］申宏波, 文景芝, 苗兴芬, 等.黑龙江省大豆新品系双抗大豆灰斑病、疫霉病鉴定［J］. 大豆科学, 2007, 26(1):107-110.(Shen H B, Wen J Z, Miao X F, et al. Identification of resistance to both Cercospor sojina and Phytophthora- root rot of new soybean lines in Heilongjiang［J］.Soybean Science, 2007, 26(1):107-110.)
［13］李修平, 韩英鹏, 丁俊杰, 等. 与耐大豆疫霉根腐病相关的QTL分析［J］.大豆科学, 2008, 27(4): 572-575.(Li X P, Han Y P, Ding J J, et al.Mapping quantitative trait loci underling tolerance to Phytophthora-root rot in soybean［J］.Soybean Science, 2008, 27(4): 572-575.)
［14］马淑梅, 丁俊杰, 郑天琪, 等. 黑龙江省大豆疫霉根腐病生理小种鉴定结果［J］.大豆科学, 2005, 24 (4): 260-262.(Ma S M, Ding J J, Zheng T Q, et al. The identification of physiological races of Phytophthora megasperma［J］.Soybean Science, 2005, 24(4): 260-262.)

相似文献/References:

[1]韩祥东,花美娜,冯永君,等.拮抗大豆疫霉菌植物内生细菌的筛选与鉴定[J].大豆科学,2012,31(01):85.[doi:10.3969/j.issn.1000-9841.2012.01.019]
　HAN Xiang-dong,HUA Mei-na,FENG Yong-jun,et al.Isolation and Characterization of Endophytic Bacteria for Phytophthora sojae Inhibition from Soybean Plant[J].Soybean Science,2012,31(02):85.[doi:10.3969/j.issn.1000-9841.2012.01.019]
[2]徐鹏飞,吴俊江,Allen G.Xue,等.大豆疫霉根腐病菌游动孢子侵染野生大豆下胚轴的透射电镜观察[J].大豆科学,2012,31(03):462.[doi:10.3969/j.issn.1000-9841.2012.03.026]
　XU Peng-fei,WU Jun-jiang,Allen G.XUE,et al.Ultrastructure of Hypocotyls of Glycine soja Infected with Zoospores of Phytophthora sojae [J].Soybean Science,2012,31(02):462.[doi:10.3969/j.issn.1000-9841.2012.03.026]
[3]孙文秀.大豆疫霉菌多聚半乳糖醛酸酶pspg1基因的克隆及表达分析[J].大豆科学,2009,28(05):781.[doi:10.11861/j.issn.1000-9841.2009.05.0781]
　SUN Wen-xiu.Molecular Cloning and Expression Analysis of a Polygalac turonase pspg1 fromPhytophthora sojae[J].Soybean Science,2009,28(02):781.[doi:10.11861/j.issn.1000-9841.2009.05.0781]
[4]左豫虎,杨传平,郑耀,等.大豆疫霉菌诱导的β-1，3-葡聚糖酶与寄主抗病性的关系[J].大豆科学,2009,28(05):883.[doi:10.11861/j.issn.1000-9841.2009.05.0883]
　ZUO Yu-hu,YANG Chuan-ping,ZHENG Yao,et al.Relationship Between Induced β-1，3-Glucanase and Resistance of Soybean to Phytophthora Root Rot[J].Soybean Science,2009,28(02):883.[doi:10.11861/j.issn.1000-9841.2009.05.0883]
[5]孙石,赵晋铭,武晓玲,等.黄淮地区大豆种质对疫霉根腐病的抗性分析[J].大豆科学,2008,27(03):465.[doi:10.11861/j.issn.1000-9841.2008.03.0465]
　SUN Shi,ZHAO Jin-ming,WU Xiaoling,et al.Resistance of Soybean Germplasm to Phytohpthora in Huanhuai Valley[J].Soybean Science,2008,27(02):465.[doi:10.11861/j.issn.1000-9841.2008.03.0465]
[6]左豫虎,于红梅,宋玉华.紫外线对大豆疫霉菌的诱导变异作用[J].大豆科学,2008,27(03):490.[doi:10.11861/j.issn.1000-9841.2008.03.0490]
　ZUO Yu-hu,YU Hong-mei,SONG Yu-hua.Induced Mutation Effect of Phytophthora sojae by Ultraviolet Irradiating[J].Soybean Science,2008,27(02):490.[doi:10.11861/j.issn.1000-9841.2008.03.0490]
[7]刘春来,李新民,杨明秀.大豆疫霉菌检测鉴定方法[J].大豆科学,2008,27(03):527.[doi:10.11861/j.issn.1000-9841.2008.03.0527]
　LIU Chun-lai,LI Xin-min,YANG Ming-xiu.Methods for Detection and Identification of Phytophthora sojae[J].Soybean Science,2008,27(02):527.[doi:10.11861/j.issn.1000-9841.2008.03.0527]
[8]许艳丽,张红骥,张匀华,等.复配生防菌株防治大豆根腐病的研究[J].大豆科学,2008,27(02):270.[doi:10.11861/j.issn.1000-9841.2008.02.0270]
　XU Yan-li,ZHANG Hong-ji,ZHANG Yun-hua,et al.Combining Biocontrol Strains against Soybean Root Rot[J].Soybean Science,2008,27(02):270.[doi:10.11861/j.issn.1000-9841.2008.02.0270]
[9]侯巨梅,刘铜,左豫虎.大豆疫霉菌生物学性状的遗传与变异[J].大豆科学,2007,26(06):918.[doi:10.3969/j.issn.1000-9841.2007.06.021]
　HOU Ju-mei,LIU Tong,ZUO Yu-hu.INHERITANCE AND VARIATION OF BIOLOGICAL CHARACTERISTICS OF PHYTOPHTHORA SOJAE[J].Soybean Science,2007,26(02):918.[doi:10.3969/j.issn.1000-9841.2007.06.021]
[10]高同国,李术娜,张冬冬,等.大豆根腐病生防细菌优势菌株的筛选、鉴定及生防效果验证[J].大豆科学,2015,34(04):661.[doi:10.11861/j.issn.1000-9841.2015.04.0661]
　GAO Tong-guo,LI Shu-na,ZHANG Dong-dong,et al.Screening and Identification of Biocontrol Dominant Bacterium against to Soybean Root Rot[J].Soybean Science,2015,34(02):661.[doi:10.11861/j.issn.1000-9841.2015.04.0661]

备注/Memo

基金项目：公益性行业（农业）专项 (201303018)；黑龙江大豆产业振兴院士工作站资助项目 (2013DYGZ203-3)；黑龙江省自然科学基金面上项目 (C201449)；黑龙江省杰出青年科学基金（JC2015005）。

第一作者简介：杨晓贺（1981-），女，硕士，助理研究员，主要从事作物病虫害防治研究。E-mail：yangxiaohe_2000@163.com。通讯作者：丁俊杰（1974-），男，博士，研究员，主要从事大豆病害研究。E-mail：me999@126.com。

更新日期/Last Update: 2016-04-04