ZHANG Hai-ping,WANG Zhi,WU Shu-feng.Correlation Analysis of Root Exudates in Resistant and Susceptible Soybean and Resistance to SCN4[J].Soybean Science,2016,35(02):180-185.[doi:10.11861/j.issn.1000-9841.2016.02.0285]
大豆胞囊线虫4号生理小种不同抗性材料根系分泌物对其抗病性的影响
- Title:
- Correlation Analysis of Root Exudates in Resistant and Susceptible Soybean and Resistance to SCN4
- Keywords:
- Soybean; Soybean Cyst Nematode; Race 4; Root exudates; Amino acid.
- 文献标志码:
- A
- 摘要:
- 为研究大豆根系分泌物与大豆对胞囊线虫4号生理小种抗性的关系，以CBL黑豆（抗）和品7514（感）为试材，收集其不同处理的根系分泌物，测定根系分泌物对胞囊线虫4号生理小种卵孵化的影响和对二龄幼虫的趋化性，并测定了根系分泌物中氨基酸成分及含量，分析了其与大豆对胞囊线虫4号生理小种抗性之间的相关性。结果表明：CBL黑豆根系分泌物能抑制线虫卵的孵化，而品7514根系分泌物能刺激卵的孵化。品7514根系分泌物对二龄幼虫趋化性影响显著大于CBL黑豆。在供试材料大豆根系分泌物中共检测出17种氨基酸。无论接种与否，品7514根系分泌物中氨基酸总量高于CBL黑豆，是CBL黑豆根系分泌物中氨基酸总量的2倍多。与不接种对照相比，接种后CBL黑豆和品7514根系分泌物氨基酸总量都表现为增加，且品7514增幅大于CBL黑豆，品7514根系分泌物中氨基酸总量增加了29729 μg?g-1FW，而CBL黑豆增加了19115 μg?g-1FW。根据氨基酸增幅及变化规律，将氨基酸分为3类：第一类为苯丙氨酸，与大豆对胞囊线虫4号小种的抗性呈正相关；第二类包含3种氨基酸，分别为苏氨酸、丝氨酸和蛋氨酸，与抗性不相关；第三类包含其余13种氨基酸，与抗性呈负相关。
- Abstract:
- In order to reveal the relationship between soybean root exudates and the resistance to the race 4 of Soybean Cyst Nematode (SCN4), root exudates were collected from resistant cultivar (CBLheidou) and susceptible cultivar (Pin 75-14).The influence of root exudates on egg incubation of SCN4 and content of free amino acids in exudates were determined.The results indicated that root exudates had an important effect on the egg hatch of SCN4. Root exudates of Pin 75-14 stimulated egg incubation of SCN4. While root exudates of CBLheidou inhibited egg incubation of SCN4.Affinity of Pin 7514rootexudates on J2 of SCN4 was higher than that of CBLheidou. Seventeen amino acids were detected in root exudates.The total amino acids content in exudates of Pin 7514 was always higher than that in exudates of CBLheidou whether they were inoculated. After inoculated, the total amino acid content of exudates in CBLheidou and Pin 7514 were both increased.The total amino acid increase of exudates in Pin 7514 and CBLheidou were 29729 and 19115 μg?g-1FW, respectively. According to amino acid increment and change regularity, amino acids were divided into three groups.The first group included phenylalanine, which was positive to soybean resistance to SCN4; the second group included threonine, serine and methionine, which were not related with soybean resistance to SCN4; the third group included others thirteen amino acids, which were negative relationship with soybean resistance to SCN4.
参考文献/References:
［1］王敬强，程大新，宛煜嵩，等．应县小黑豆对大豆孢囊线虫4 号生理小种抗性的遗传分析［J］．中国农学通报，2001，17（6）：12-15．(Wang J Q，Cheng D X, Wan Y S, et al． Genetic analyzing of resistance to race 4 of soybean cyst nematode in soybean ZDD2226 ［J］. Chinese Agricultural Science Bulletin, 2001，17(6): 12-15)［2］卢为国, 盖钧镒, 李卫东. 黄淮地区大豆胞囊线虫生理小种的抽样调查与研究［J］.中国农业科学, 2006, 39(2):306-312．(Lu W G, Gai J Y, Li W D.Sampling survey and identification of races of soybean cyst nematode (Heterodera glycines Ichinohe) in Huang-Huai Valleys［J］ Scientia Agriuclura Sinica, 2006, 39(2):306-312.)［3］孙漫红, 刘杏忠, 缪作清. 大豆胞囊线虫病生物防治研究进展［J］.中国生物防治, 2000, 16(3): 136-141. (Sun M H, Liu X Z, Miu Z Q. Biological control of soybean cyst nematode ［J］. Chinese Journal of Biological Control, 2000, 16(3): 136-141.)［4］韩雪, 吴凤芝, 潘凯．根系分泌物与土传病害关系之研究综述［J］．植物保护科学，2006，22（2）：316-318．(Han X, Wu F Z, Pan K.Review on the relation between the root exudates and soil- spread disease［J］. Chinese Agricultural Science Bulletin, 2006, 22(2): 316-318.)［5］袁虹霞， 李洪连， 王烨，等.棉花不同抗性材料根系分泌物分析及其对黄萎病菌的影响［J］. 植物病理学报，2002, 32（2）：127-131.(Yuan H X, Li H L, Wang Y, et al. The root exudate of cotton cultivars with the different resistance and the effect on Verticillium dahliae ［J］.Acta Phytopathologica Sinica, 2002, 32(2): 127-131.)［6］陈捷胤，戴小枫.棉花对黄萎病的抗病机制研究进展［J］.分子植物育种, 2005, 3(3): 427-435.(Chen J Y, Dai X F.Research advance on the resistant mechanism of cotton against Verticillium wilt ［J］.Molecular Plant Breeding, 2005, 3(3): 427-435.)［7］张俊英，王敬国，许永利．大豆根系分泌物中氨基酸对根腐病菌生长的影响［J］．植物营养与肥料学报,2008，14（2）：308-315．(Zhang J Y, Wang J G, Xu Y L. Effect of amino acid from soybean root exudates on hyphal growth of pathogenic fungi of soybean root rot ［J］Plant Nutrition and Fertilizer Science, 2008, 14(2): 308-315)［8］Sikora E J, Noel G R.Hatch and emergence of Heterodera glycines in root leachate from resistant and susceptible soybean cultivars ［J］. Journal of Nematology, 1996, 28(4): 501-509［9］Caballero L G, Osmanski E A, Tefft P M. Factors influencing plant-induced egg hatching in Heterodera glycines ［J］ Journal of Nematology, 1986, 18: 636［10］刘晔，刘维志．大豆胞囊线虫在不同大豆材料根内的发育［J］．辽宁农业科学，1988(4):16-18．(Liu Y, Liu W Z. Development of soybean cyst nematode within roots soybean varienties ［J］. Liaoning Agricultural Sciences, 1988(4): 16-18.)［11］Schmitt D P, Riggs R D. Influence of selected plant species on hatching of eggs and development of juveniles of Heterodera glycines ［J］.Journal of Nematology, 1991, 23(1): 1-6［12］颜清上，陈品三，王连铮.大豆根渗出物对大豆孢囊线虫4号生理小种卵孵化的影响［J］．植物病理学报，1997, 27(3): 269-274. (Yan Q S, Chen P S, Wang L Z. The effect of soybean root diffusates on the hatching of Heterodera glycines race 4 ［J］. Acta Phytopathologica Sinica,1997, 27(3): 269-274.)［13］王雪，段玉玺，陈立杰，等．大豆根系分泌物氨基酸组份与抗大豆胞囊线虫的相关性研究［J］．沈阳农业大学学报，2008，39（6）：677-681. (Wang X, Duan Y X, Chen L J，et al. Correlation analysis of amino acids components in soybean cultivars root exudates and resistance to soybean cyst nematode［J］. Journal of Shenyang Agricultural University, 2008, 39(6): 677-681.)［14］颜清上，王连铮，陈品三．中国小黑豆抗源对大豆孢囊线虫4号生理小种抗病的生化反应［J］．作物学报，1997，23(5):529-537.(Yan Q S, Wang L Z, Chen P S. Biochemical responses of resistance to race 4 of Heterodera glycines in Chinese black soybean ［J］. Acta Agronomica Sinica, 1997, 23(5): 529-537.) ［15］卢为国, 李金英，文自翔．一种大豆胞囊线虫抗性鉴定方法: 中国，CN 102246662 B［P］ 2011-11-23 (Lu W G, Li J Y, Wen Z X A method of soybean cyst nematode resistance identification: China, CN 102246662 B［P］.2011-11-23)［16］Tefft P, Mbone L W. Plant induced hatching of eggs of soybean cyst nematode Heterodera glycines ［J］. Journal Nematology, 1985, 17(3): 275-279．［17］孙漫红，刘杏忠．淡紫拟青霉发酵滤液对大豆胞囊线虫趋化性的影响［J］．植物病理学报，2004，34（4）：376-379．(Sun M H, Liu X Z. Effects of Paecilomyces lilacinus M214 fermentation filtrate on the affinity between soybean cyst nematode and soybean root ［J］ Acta Phytopathologica Sinica, 2004, 34(4): 376-379.)［18］吴海燕，段玉玺，李秀侠，等．SCN侵染对不同大豆材料根系氨基酸/低份子肽类分泌物的影响［J］．植物病理学报，2007，37（6）：616-622．(Wu H Y, Duan Y X, Li X X, et al. Amino acids / low molecular weight peptides in root exudation of different soybean cultivars resistance to Heterodera glycines［J］.Acta Phytopathologica Sinica, 2007，37(6): 616-622.)［19］蒋玉蓉，房卫平，祝水金，等．陆地棉植株组织结构和生化代谢与黄萎病抗性的关系［J］．作物学报，2005，31（3）：337-341．(Jiang Y R, Fang W P, Zhu S J, et al. Relationship of verticillium wilt resistance with plant anatomical structure and biochemical metabolism in upland cotton［J］.Acta Agronomic Sinica, 2005, 31(3): 337-341.)［20］潘凯, 吴凤芝.枯萎病不同抗性黄瓜根系分泌物氨基酸组分与抗病的相关性［J］. 生态学报, 2007, 27(5): 1945-1950. (Pan K, Wu F Z .Correlation analysis of amino acids component Fusarium wilt resistance ［J］.Acta Ecological Sinica, 2007, 27(5): 1945-1950.)［21］吴玉香, 沈晓佳, 房卫平, 等. 陆地棉根系分泌物对黄萎病菌生长发育的影响［J］.棉花学报, 2007, 19 (4): 286-290. (Wu Y X, Shen X J, Fang W P.The effects of cotton root exudates on growth and development of Verticillium dahliae［J］. Cotton Science, 2007, 19 (4): 286-290.)［22］马俊彦，杨汝德，敖利刚．植物苯丙氨酸解氨酶的生物学研究进展［J］．现代食品科技，2007，23（7）：71-74．(Ma J Y, Yang R D, Ao L G.Progress in biological research of phenylanlanine ammonialyase［J］.Modern Food Science and Technology, 2007, 23(7): 71-74.)［23］张海平，王志，李原萍．兴县灰皮支黑豆抗大豆胞囊线虫4号生理小种的生化机制研究［J］. 大豆科学，2012，31（5）：796-800.(Zhang H P, Wang Z, Li Y P. Biochemical mechanism of Xingxianhuipizhi resistant to race 4 of soybean cyst ［J］. Soybean Science, 2012, 31(5): 796-800.)
相似文献/References:
[1]李 凯,刘志涛,李海朝,等.国家大豆区域试验品种对SMV和SCN的抗性分析[J].大豆科学,2013,32(05):670.[doi:10.11861/j.issn.1000-9841.2013.05.0670]
LI Kai,LIU Zhi-tao,LI Hai-chao,et al.Resistance to Soybean Mosaic Virus and Soybean Cyst Nematode of Soybean Cultivars from China National Soybean Uniform Trials[J].Soybean Science,2013,32(02):670.[doi:10.11861/j.issn.1000-9841.2013.05.0670]
[2]郑雅楠,陈乐,陈井生,等.休眠期大豆胞囊线虫体内关键酶活性变化[J].大豆科学,2013,32(04):526.[doi:10.11861/j.issn.1000-9841.2013.04.0526]
ZHENG Ya-nan,CHEN Le,CHEN Jing-sheng,et al.Changes of Related Enzyme Activities in Dormant Soybean Cyst Nematode,Heterodera glycines[J].Soybean Science,2013,32(02):526.[doi:10.11861/j.issn.1000-9841.2013.04.0526]
[3]李泽宇,李肖白,陈井生,等.大豆品种（系）抗大豆胞囊线虫14号生理小种的抗性鉴定研究[J].大豆科学,2014,33(03):408.[doi:10.11861/j.issn.1000-9841.2014.03.0408]
LI Ze-yu,LI Xiao-bai,CHEN Jing-sheng,et al.Identification of Soybean Varieties for Resistance to Soybean Cyst Nematode Races 14[J].Soybean Science,2014,33(02):408.[doi:10.11861/j.issn.1000-9841.2014.03.0408]
[4]袁翠平,赵洪锟,王玉民,等.利用SSR标记评价抗胞囊线虫野生大豆种质的遗传多样性[J].大豆科学,2014,33(02):147.[doi:10.11861/j.issn.1000-9841.2014.02.0147]
YUAN Cui-ping,ZHAO Hong-kun,WANG Yu-min,et al.Genetic Diversity of Wild Soybean(Glycine soja)Resistant Germplasms to Soybean Cyst Nematode Revealed by SSR Markers[J].Soybean Science,2014,33(02):147.[doi:10.11861/j.issn.1000-9841.2014.02.0147]
[5]李晓明,杜春梅,郑楠,等.芽孢杆菌BL-21、HNDF2对大豆胞囊线虫抑制效果的研究[J].大豆科学,2011,30(04):710.[doi:10.11861/j.issn.1000-9841.2011.04.0710]
LI Xiao-ming,DU Chun-mei,ZHENG Nan,et al.Inhibitory Effect of Bacillus BL-21,HNDF2 on Soybean Cyst Nematode[J].Soybean Science,2011,30(02):710.[doi:10.11861/j.issn.1000-9841.2011.04.0710]
[6]郑雅楠,王媛媛,陈井生,等.大豆胞囊线虫热激蛋白基因Hsp70的克隆与原核表达[J].大豆科学,2012,31(02):198.[doi:10.3969/j.issn.1000-9841.2012.02.008]
ZHENG Ya-nan,WANG Yuan-yuan,CHEN Jing-sheng,et al.Cloning and Prokaryotic Expression of Hsp70 from Soybean Cyst Nematode(Heterodera glycines)[J].Soybean Science,2012,31(02):198.[doi:10.3969/j.issn.1000-9841.2012.02.008]
[7]刘丹丹,段玉玺,陈立杰.酸类化合物对大豆胞囊线虫防效及寄主生长的影响[J].大豆科学,2012,31(02):278.[doi:10.3969/j.issn.1000-9841.2012.02.024]
LIU Dan-dan,DUAN Yu-xi,CHEN Li-jie.Effect of Acid Compound on Soybean Cyst Nematode and Host Growth[J].Soybean Science,2012,31(02):278.[doi:10.3969/j.issn.1000-9841.2012.02.024]
[8]胡 新,许艳丽,LI Shu-xian,等.利用抗感品种混种防治大豆胞囊线虫效果的研究[J].大豆科学,2012,31(03):449.[doi:10.3969/j.issn.1000-9841.2012.03.023]
HU Xin,XU Yan-li,LI Shu-xian,et al.Effect of Cultivar Mixture on Growth and Developoment of Soybean Inoculated with Soybean Cyst Nematode[J].Soybean Science,2012,31(02):449.[doi:10.3969/j.issn.1000-9841.2012.03.023]
[9]宋洁,许艳丽,姚钦,等.尿素对大豆胞囊线虫的抑制作用[J].大豆科学,2012,31(05):784.[doi:10.3969/j.issn.1000-9841.2012.05.020]
SONG Jie,XU Yan-li,YAO Qin,et al.Suppression of Urea on Soybean Cyst Nematode[J].Soybean Science,2012,31(02):784.[doi:10.3969/j.issn.1000-9841.2012.05.020]
[10]胡海波,韩英鹏,滕卫丽,等.重组自交系群体对大豆胞囊线虫3号生理小种抗性与主要农艺性状的相关分析[J].大豆科学,2012,31(05):793.[doi:10.3969/j.issn.1000-9841.2012.05.022]
HU Hai-bo,HAN Ying-peng,TENG Wei-li,et al.Correlation between Resistance to Soybean Cyst Nematode Race No.3 and Major Agronomic Traits of Recombinant Inbred Lines[J].Soybean Science,2012,31(02):793.[doi:10.3969/j.issn.1000-9841.2012.05.022]
备注/Memo
基金项目：山西省农业科学院育种基础项目（yyzjc1304）；山西省科技厅自然基金（2015011073）；农业部作物种质资源保护子项目（2015NWW030-14-21）；山西省农业科学院博士后项目（BHS-2015JJ-001）。第一作者简介：张海平（1978-），女，博士，副研究员，主要从事大豆种质资源研究。E-mail:nkyzhp@126.com。