XU Peng-fei,WU Jun-jiang,Allen G.XUE,et al.Ultrastructure of Hypocotyls of Glycine soja Infected with Zoospores of Phytophthora sojae [J].Soybean Science,2012,31(03):462-465.[doi:10.3969/j.issn.1000-9841.2012.03.026]
大豆疫霉根腐病菌游动孢子侵染野生大豆下胚轴的透射电镜观察
- Title:
- Ultrastructure of Hypocotyls of Glycine soja Infected with Zoospores of Phytophthora sojae
- 文章编号:
- 1000-9841（2012）03-0462-04
- Keywords:
- Phytophthora sojae; Wild soybean; Ultrastructure
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 用大豆疫霉根腐病菌的游动孢子悬浮液处理抗感性不同的野生大豆下胚轴，接种后3～72 h，用透射电镜观察野生大豆与大豆疫霉菌的亲和性与非亲和性互作中细胞超微结构的变化。结果表明：接种后3～36 h，感病野生大豆随接种时间延长下胚轴细胞器结构破坏严重，而抗病野生大豆细胞结构基本完整；接种后48 h，抗病野生大豆的细胞壁物质累加，出现胞壁沉积物，而感病野生大豆细胞壁出现部分降解；接种后72 h，抗病野生大豆细胞质壁分离，但细胞器结构基本完整，感病野生大豆细胞器几乎无完整的结构。
- Abstract:
- The ultrastructures of hypocotyls of resistant and susceptible wild soybeans infected with zoospores of Phytophthora sojae from 3 to 72 h after inoculation were observed using transmission electron microscopy in this paper. The results showed that the cell structures of the susceptible wild soybean were seriously destroyed with the inoculation time from 3 to 36 h, but those of the resistant one were almost intact. The sediments on the cell wall of the resistant soybean could be seen at 48 h after inoculation, but no sediments could be seen for the susceptible one and its cell wall began to degrade to some extent. Although there was plasmolysis at 72 h after inoculation, the cell structures were almost intact for the resistant wild soybean. However, there were nearly no intact cell structures for the susceptible ones. The results could provide theoretical basis on cytology for clarification of pathogenic mechanism of P. sojae to wild soybeans.
参考文献/References:
[1]Schmitthenner A F.Problems and progress in control of
Phytophthora
?root rot of soybean[J].Plant Disease,1985,69(4):362-368.
[2]沈崇尧,苏彦纯.中国大豆疫霉菌的发现及初步研究[J].植物病理学报,1991,21(3):298.（Shen C Y,Su Y C.Discovery and preliminary studies of Phytophthora megaspermaon soybean in China[J].Acta Phytopathologica Sinica,1991,21(3):298.）
[3]韩晓增,何志鸿,张增敏.大豆主要病虫害防治技术[J].大豆通报,1998(6):5-6.（Han X Z,He Z H,Zhang Z M.The prevention technique of main disease and pests of soybean[J].Soybean Bulletin,1998(6):5-6.）
[4]朱振东,王晓鸣,常汝镇,等.黑龙江省大豆疫霉菌生理小种鉴定及大豆种质的抗性评价[J].中国农业科学,2000,33(1):62-67.（Zhu Z D,Wang X M,Chang R Z,et al.Identification of race of Phytophthora sojaeand reaction of soybean germplasm resources in Heilongjiang province[J].Scientia Agricultura Sinica,2000,33(1):62-67.） [5]Zhang S Z,Xu P F,Wu J J,et al.Races of?Phytophthora sojae?and their virulences on commonly grown soybean varieties in Heilongjiang,China[J].Plant Disease,2010,94:87-91.
[6]Enkerli K,Mims C W,Hahn M G.Ultrastructure of compatible and incompatible interactions of soybean roots infected with the plant pathogenic oomycete
Phytophthora sojae
[J]. Canadian Journal of Botany,1997,75(9):1493-1508.
[7]左豫虎,康振生,黄丽丽,等.大豆疫霉菌对大豆下胚轴侵染过程的细胞学研究[J].植物病理学报,2005,35(3):235-241.（Zuo Y H,Kang Z S,Huang L L,et al.Cytology on infection process of soybean hypocotyls by Phytophthora sojae[J].Acta Phytopathologica Sinica,2005,35(3):235-241.） [8]Beagle-Ristaino J E,Rissler J F.Histopathology of susceptible and resistant soybean roots inoculated with zoospores of Phytophthora megaspermaf.sp.glycinea[J].Phytopathology,1983,73:590-595.
[9]葛秀秀.大豆抗疫霉根腐病机制的初步研究[D].哈尔滨：东北农业大学，2001.（Ge X X.Study on resistance mechanism of soybeans infected by Phytophthora sojae[D]. Harbin：Northeast Agricultural University,2001.）
[10]Yang X B.Races of
Phytophthora sojea
in Iowa soybean fields[J].Plant Disease，1996,80:1418-1420.
[11]左豫虎,臧忠婧,刘惕若.影响大豆疫霉菌游动孢子产生的条件[J].植物病理学报,2001,31(3):241-245.（Zuo Y H,Zang Z J,Liu T R.Stusies on production condition of zoospores ofPhytophthora sojae[J].Acta Phytopathologica Sinica,2001,31(3):241-245.）
相似文献/References:
[1]高越,刘辉,陶波.抗草甘膦野生大豆筛选及其抗性生理机制研究[J].大豆科学,2013,32(01):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
GAO Yue,LIU Hui,TAO Bo.Screening and Physiological Mechanisms of Resistance to Glyphosate in Wild Soybeans(Glycine soja)[J].Soybean Science,2013,32(03):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
[2]王军卫,侯立江,李? 登,等.野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
WANG Jun-wei,HOU Li-jiang,LI Deng,et al.Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(03):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
[3]王军卫,侯立江,李 登,等. 野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.
WANG Jun-wei,HOU Li-jiang,LI Deng,et al. Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(03):596.
[4]王丽燕.硅对野生大豆幼苗耐盐性的影响及其机制研究[J].大豆科学,2013,32(05):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
WANG Li-yan.Effects of Silicon on Salt Tolerance of Glycine soja Seedlings and Its Mechanism[J].Soybean Science,2013,32(03):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
[5]陈丽丽,王明玖,何丽君,等.野生大豆ISSR体系的优化及其在远缘杂交后代鉴定中的利用[J].大豆科学,2013,32(04):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
CHEN Li-li,WANG Ming-jiu,HE Li-jun,et al.Optimization for ISSR Reaction System of Wild Soybean and Its Utilization in Distant Hybrid Identification[J].Soybean Science,2013,32(03):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
[6]郑世英,萧蓓蕾,金桂芳.NaCl胁迫对野生大豆和栽培大豆叶绿素及光合特性的影响[J].大豆科学,2013,32(04):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
ZHENG Shi-ying,XIAO Bei-lei,JIN Gui-fang.Effect of NaCl Stress on Chlorophyll Content and Photosynthetic Characteristics of Glycine soja and Glycine max[J].Soybean Science,2013,32(03):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
[7]徐艳平,胡翠美,张文会,等.干旱胁迫对野生大豆幼苗光合作用相关指标的影响[J].大豆科学,2013,32(03):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
XU Yan-ping,HU Cui-mei,ZHANG Wen-hui,et al.Effect of Simulated Drought Stress on Photosynthesis Related Indexes at Seedling Stage of Wild Soybeans[J].Soybean Science,2013,32(03):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
[8]胡卫静,何丽君,何劲莉,等.NaCl胁迫对野生与栽培大豆杂交后代株系生理指标的影响[J].大豆科学,2013,32(03):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
HU Wei-jing,HE Li-jun,HE Jin-li,et al.Effects of NaCl Stress on Physiological Characters of Soybean Hybrids from Glycine max × Glycine soja[J].Soybean Science,2013,32(03):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
[9]王 旻,梁 玉,王欣欣,等.即墨野生大豆主要成分及其营养价值分析[J].大豆科学,2013,32(03):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
WANG Min,LIANG Yu,WANG Xin-xin,et al.Assessment on Nutritional Compositions and Value of Jimo Wild Soybean[J].Soybean Science,2013,32(03):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
[10]程鹏,徐鹏飞,范素杰,等.野生大豆接种大豆疫霉根腐病菌后过氧化物酶（POD）活性变化[J].大豆科学,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
CHENG Peng,XU Peng-fei,FAN Su-jie,et al.Response of POD Activity in Glycine soja ?Inoculated by Phytophthora sojae[J].Soybean Science,2013,32(03):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
[11]孙晓慧,徐鹏飞,张淑珍.大豆疫霉根腐病菌游动孢子侵染野生大豆下胚轴的细胞学观察[J].大豆科学,2017,36(03):430.[doi:10.11861/j.issn.1000-9841.2017.03.0430]
SUN Xiao-hui,XU Peng-fei,ZHANG Shu-zhen.Cytological Observation on Infection Process of Hypocotyls of Wild Soybeans by Zoospore of Phytophthora sojae[J].Soybean Science,2017,36(03):430.[doi:10.11861/j.issn.1000-9841.2017.03.0430]
备注/Memo
基金项目：国家自然科学基金（309T1811，31071439，31171577，31110103001），教育部新世纪人才培养计划（NCET-09-164）；黑龙江省自然科学基金（C200814）；黑龙江省博士后养基金（ORB06-010）；黑龙江省教育厅科学技术研究项目（12511041）。