[1]吕美云,刘紫英.豆豉中5株产纤溶酶菌的筛选与鉴定[J].大豆科学,2016,35(01):165-170,180.[doi:10.11861/j.issn.1000-9841.2016.01.0165]
　LYU Mei-yun,LIU Zi-ying.Isolation and Identification of Five Fibrinolytic Enzyme Producing Strains from Douchi（Chinese Traditional Food）[J].Soybean Science,2016,35(01):165-170,180.[doi:10.11861/j.issn.1000-9841.2016.01.0165]
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豆豉中5株产纤溶酶菌的筛选与鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年01期 页码: 165-170,180 栏目: 出版日期: 2016-01-20
Title:
Isolation and Identification of Five Fibrinolytic Enzyme Producing Strains from Douchi（Chinese Traditional Food）
作者:
吕美云; 刘紫英
宜春学院 化学与生物工程学院，江西 宜春 336000
Author(s):
LYU Mei-yun;  LIU Zi-ying
College of Chemistry and Bioengineering of Yichun University，Yichun 336000，China
关键词:
纤溶酶; 筛选; 16S rDNA; 鉴定; 枯草芽孢杆菌
Keywords:
Fibrinolytic enzyme; Screened; 16S rDNA; Identification; Bacillus subtilis
DOI:
10.11861/j.issn.1000-9841.2016.01.0165
文献标志码:
A
摘要:
采用酪蛋白平板初筛的方法，从全国14种纳豆和豆豉中分离到39株具有蛋白酶活力的菌株；又以猪血粉平板进行复筛，并采用纤维蛋白平板法测定纤溶酶活力，得到5株产纤溶酶能力较强菌株，分别命名为CQDC-03、CWND-03、NCDC-02、YJND-01和FJDC-02。对其进行16S rDNA序列分析，结果表明：CWND-03、CQDC-03和Bacillus subtilis相似性为99%，NCDC-02和Bacillus subtilis相似性为98%。因而CQDC-02、CWND-03和NCDC-02认定为Bacillus subtilis，YJND-01和FJDC-02是否为Bacillus subtilis的新种还有待进一步鉴定，但确定其为Bacillus属
Abstract:
Thirty-nine strains of protease-producing strains were isolated from 14 kinds of traditional food natto and Douchi by using casein-plate method and 5 strains of highest fibrinolytic enzyme producing strains were screened by both pig blood powder-plate and fibrin plate method. The 5 strains were named NCDC-2,CQDC-03,CWND-03,YJND-01 and FJDC-02,respectively 16S rDNA sequence analysis showed NCDC-02 was similary with.Bacillus subtilis by 98%, while CQDC-03 and CWND-03 were by 99%. Thus NCDC-2, CQDC-03, CWND-03 were identified as Bacillus subtilis Further research is needed to identify whether YJND-01 and FJDC-02 are the new species of Bacillus subtilis. But it is no doubt that it is kind of Bacillus-spp

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备注/Memo

基金项目：江西省宜春市社会发展类科技计划（JXYC2011KSA002）。

第一作者简介：吕美云（1963-），女，副教授，主要从事微生物学教学与研究。E-mail：lvmeiyun2005@163.com。

更新日期/Last Update: 2016-02-05