[1]高赛男,赵雪,李文滨,等.农家大豆种质对花叶病毒病N1和N3株系的抗性鉴定[J].大豆科学,2016,35(01):117-123.[doi:10.11861/j.issn.1000-9841.2016.01.0117]
　GAO Sai-nan,ZHAO Xue,LI Wen-bin,et al.Identification of Resistance of Soybean Landraces to N1 and N3 Strains of Soybean Mosaic Virus[J].Soybean Science,2016,35(01):117-123.[doi:10.11861/j.issn.1000-9841.2016.01.0117]
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农家大豆种质对花叶病毒病N1和N3株系的抗性鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年01期 页码: 117-123 栏目: 出版日期: 2016-01-20
Title:
Identification of Resistance of Soybean Landraces to N1 and N3 Strains of Soybean Mosaic Virus
作者:
高赛男; 赵雪; 李文滨; 李海燕; 韩英鹏
东北农业大学 大豆生物学教育部重点实验室/农业部北方大豆生物学与遗传育种区域重点实验室，黑龙江 哈尔滨 150030
Author(s):
GAO Sai-nan; ZHAO Xue; LI Wen-bin; LI Hai-yan ; HAN Ying-peng
Chinese Education Ministry’s Key Laboratory of Soybean Biology/Key Laboratory of Northeastern Soybean Biology and Breeding Genetics of Chinese Agriculture Ministry, Northeast Agricultural University,Harbin 150030, China
关键词:
大豆种质; 花叶病毒病;  抗性鉴定; 农家品种
Keywords:
Germplasm; Soybean; Landrace; Resistance identification
DOI:
10.11861/j.issn.1000-9841.2016.01.0117
文献标志码:
A
摘要:
农家品种作为重要的种质资源，其抗病性鉴定对大豆遗传育种材料的选择至关重要。采用摩擦接种法对46份农家大豆种质进行N1和N3株系的抗性鉴定。结果表明：对N1株系表现高抗的农家种质为 6份,分别是铁荚子、天鹅蛋、青仁黑豆、黑豆、大青仁和冬豆； 对N3株系表现高抗的农家种质为6份,分别是铁荚子、天鹅蛋、青仁黑豆、黑豆、化眉豆和小白脐；对N1和N3株系均表现高抗的种质为4 份，分别是铁荚子、天鹅蛋、青仁黑豆、黑豆。这为SMV抗性育种奠定了材料基础。利用前期研究获得的与大豆花叶病毒病抗性基因相关的SSR标记Satt114，进行分子辅助鉴定， 46份农家种质通过Satt114分子辅助鉴定，共筛选出8份抗大豆花叶病毒病种质，分别是铁荚子、黑豆、天鹅蛋、大青仁、青仁黑豆、冬豆、丰地黄、小白脐，其中丰地黄和小白脐的鉴定结果与摩擦接种法的鉴定结果不符，需进一步试验鉴定。
Abstract:
As an important germplasm resource, landrace is very important for the material selection of soybean genetic breeding. In this paper, 46 landraces were friction inoculated with N1 and N3 strains to select SMV resistant germplasm. The results showed that there were six landraces resistant to SMV N1 including Tiejiazi, Tianedan, Qingrenheidou, Heidou, Daqingren and Dongdou. Six landraces were resistant to SMV N3, they were Tiejiazi, Tianedan, Qingrenheidou, Heidou, Huameidou and Xiaobaiqi. A total of 4 landraces were resistance to both SMV N1 and SMV N3, which laid the material foundation for breeding resistant cultivars. Molecular assisted identification utilizing SSR markers Satt 114 related with resistance gene obtained by previous studies.The results showed that a total of 8 landraces were resistant to SMV of which Fengdihuang and Xiaobaiqi were reverified through friction inoculation test and further test and identification should be done.

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备注/Memo

基金项目：黑龙江省普通高等学校新世纪优秀人才培养计划（1253-NCET-005）。

第一作者简介：高赛男(1989-)，女，硕士，主要从事分子辅助育种研究。E-mail：1043288953@qq.com。
通讯作者：韩英鹏（1978-），男，博士，教授，主要从事大豆遗传育种和分子生物技术研究。E-mail: hyp234286@aliyun.com。

更新日期/Last Update: 2016-02-05