GAO Hua wei,MAN Qiang,WU Meng,et al.Screening of Soybean Germplasm with Good Seed Longevity[J].Soybean Science,2014,33(01):6-12.[doi:10.11861/j.issn.1000-9841.2014.01.0006]
耐储藏大豆种质资源的筛选
- Title:
- Screening of Soybean Germplasm with Good Seed Longevity
- 文章编号:
- 1000-9841（2014）01-0006-07
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 耐储藏大豆种质的筛选是大豆种子耐储藏性遗传改良的基础。通过测定种子发芽率和电导率等指标，对87份大豆种子的耐储藏性进行评价。结果共筛选出耐储藏大豆种质38份，包括24份地方种质和14份栽培种质。相关分析表明，大豆种子的耐储藏性与种皮色素沉积量正相关，与大豆籽粒大小负相关。但在这些耐储藏种质中，20份种质的种皮为黄色，3份种质的百粒重超过22 g；大豆种子的耐储藏性与电导率负相关，但并不显著，电导率只能作为评价大豆耐储藏性的参考指标。所筛选出的38份种质可直接用于大豆种子耐储藏性的遗传改良，为进一步研究大豆种子耐储藏性的遗传机理奠定材料基础。
- Abstract:
- Germplasm with good seed longevity underlie the genetic improvement for seed longevity in soybean.Thirty eight out of 87 germplasm with good longevity were screened according to the germination rate,total dissolved solids(TSD)and other indexes,including 24 landraces and 14 cultivars.The results showed that the seed longevity was positive related with the pigment content and negative related with the seed size.While among the germplasm with good longevity,20 ones bore yellow seed coats and three ones possessed big seed with 100 seed weight over 22 g.The relationship between seed longevity and TSD was negative but not significantly,therefore,TSD could be used as reference index to evaluate soybean seed longevity.These elite germplasm with good seed longevity could be directly used in breeding progress and they provide genetic materials for seed longevity researches.
参考文献/References:
[1]Dao P,Ram H H.大豆种子寿命的遗传[J].大豆科学，1999，18（4）：312-317.(Dao P,Ram H H.Genetics of seed longevity in soybean[J].Soybean Science,1999,18(4):312-317.
[2] 乔燕祥，周建萍，田齐建，等.大豆种子老化过程中生理特性变化的研究[J].植物遗传资源学报，2010，11（5）：616 620.（Qiao Y X,Zhou J P,Tian Q J,et al.Changing of physiological characteristics of soybean seeds in aging course[J].Journal of Plant Genetic Resources,2010,11（5）:616 620.）
[3]Shelar V R,Shaikh R S,Nikam A S.Soybean seed quality during storage:a review[J].Agricultural Reviews,2008,29:125 131.
[4]Emile J M,Mohamed E E,Elizabeth V,et al.Analysis of natural allelic variation of Arabidopsis seed germination and seed longevity traits between the accessions ?Landsberg erecta and Shakdara ,using a new recombinant inbred line population[J].Plant Physiology,2004,135:432 443.
[5]崔聪淑，卢新雄.八个大豆品种耐藏性初探[J].作物品种资源，1992（3）：40 41.(Cui C S,Lu X X.Preliminary research on seed longevity of eight soybean varieties[J].China seeds,1992(3):40 41.)
[6]任冲.大豆种子储藏特性及其相关衰老机理研究[D].中国科学院研究生院，2010.（Ren C.The research of soybean seed storage characteristics and related physiological mechanism[D].Graduate school of Chinese Academy of Sciences,2010.）
[7]刘春双.大豆在储藏期间的品质变化[J].中国油脂，2000，34(12)：6567.(Liu C S.Quality change of soybean during the period of storage[J].China Oils and Fats,2009,34(12):65-67.)
[8]Wien H C,Kueneman E A.Soybean seed deterioration in the tropics.II.Varietal differences and techniques for screening[J].Field Crops Research,1981,4:123-132.
[9]GB/T3543.4.农作物种子检验规程发芽试验[S].北京:中国标准出版社,1995.(GB/T3543.4.Rules for agricultural seeds testing Germination test[S].Beijing:China Standards Press,1995.)
[10]GB/T5520 2011.粮油检验 种子发芽试验[S].北京:中国标准出版社,2011.(Inspection of grain and oils Germination test of seeds[S].Beijing:China Standards Press,2011.)
[11]Rajjou L,Debeaujon I.Seed longevity:survival and maintenance of high germination ability of dry seeds[J].Comptes Rendus Biologies,2008,10:796-805.
[12]GB4404.2粮食作物种子第2部分:豆类[S].北京:中国标准出版社,2010.（GB4404.2.seed of food crops Part2:Legume.[S].Beijing:China Standards Press,2010.）
[13]徐本美，韩建国，浦心春.种苗评定与种子活力测定方法手册[M].北京:北京农业大学出版社，1993:101 128.（Xu B M,Han J G,Pu X C.Handbook of seedlings evaluation and seed vigour test methods[M].Beijing:Beijing Agricultural University Press,1993:101 128.）
[14]Cerabolini B,Ceriani R M,Caccianiga M,et al.Seed size,shape and persistence in soil:a test on Italian flora from Alps to Mediterranean coasts[J].Seed Science Research,2003,13:75 85.
[15]Edwards C J,Hartwig E E.Effect of seed size upon rate of germination in soybeans[J].Agronomy Journal,1971,63:429 450.
[16]肖伶俐，康玉凡，陶礼明，等.不同大豆品种芽用特性比较[J].大豆科学，2008，27(6)：955 959.(Xiao L L,Kang Y F,Tao L M,et al.Comparison analysis of characteristics of Soybean growing sprouts for different soybean varieties[J].Soybean Science,2008,27(6):955 959.)
[17]Starzinger E K,West S H,Hinson K.An observation on the relationship of soybean seed coat color to viability maintenance [J].Seed Science and Technology,1982,10:301-305.
[18]Tully R E,Musgrave M E,Leopold A C.The seed coat as a control of imbibitional chilling injury[J].Crop Science,1981,21:312 317.?
[19]Mohamed Yasseen Y,Barringer S A,Splittstoesser W E,et al.The role of seed coats in seed viability[J].The Biotanical Review,1994,60:426 439.
[20]周建萍，乔燕祥，穆志新，等.大豆种子老化过程中活力指标的研究[J].山西农业科学，2007，35(3)：33 35.(Zhou J P,Qiao Y X,Mu Z X,et al.Study on vitality index of soybean seed in aging process[J].Journal of Shanxi Agricultural Sciences,2007,35(3):33 35.)
[21]杨亚平，姜孝成，陈良碧，等.水稻种子老化的生理机制[J].湖南农业大学学报:自然科学版，2008，34(3):265 269．(Yang Y P,Jiang X C,Chen L B,et al.Study on physiological mechanism in aging of rice seeds[J].Journal of Hunan Agricultural University (Natural Sciences)2008,34(3):265 269.)
[22]颜启传.种子学[M].北京：中国农业出版社，2007.(Yan Q C.Spermology[M].Beijing:China Agriculture Press,2007.)
[23]张文明，郑文寅，任冲，等.电导法测定大豆种子活力的初步研究[J].种子，2003(2)：34 36，38.（Zhang W M,Zheng W Y,Ren C,et al.Study on testing method of seed vigor by electrical conductivity in soybean[J].Seed,2003(2)：34-36,38.）
[24]张洁，郭数进，马金虎.两个大豆品种在人工加速老化过程中种子发芽和活力的变化[J].山西农业大学学报（自然科学版），2009，29(3):198 201.(Zhang J,Guo S J,Ma J H.Changes of seeds’ germination and activity of two soybean varieties in artificially accelerated aging[J].Journal of Shanxi Agricultural University (Natural Science Edition),2009,29(3):198 201.)
[25]毕辛华，戴心维.种子学[M].北京:中国农业出版社，1993.(Bi X H,Dai X W.Spermology[M].Beijing:China Agriculture Press,1993.)
[26]Parrish D J,Leopold A C.On the mechanism of aging in soybean seeds[J].Plant Physiology,1978,61:365-368.
[27]刘明久，王铁固，陈士林，等.玉米种子人工老化过程中生理特性与种子活力的变化[J].核农学报，2008，22(4):510 513．(Liu M J,Wang T G,Chen S L,et al.Physioloycial and seed vigor changes of maize seeds during artificial aging course[J].Journal of Nuclear Agricultural Sciences,2008,22(4):510 513.)
[28]Moreano T B,Braccini A L,Scapim C A,et al.Changes in the effects of weathering and mechanical damage on soybean seed during storage[J].Seed Science and Technology,2011,39(3):604 611.
[29]汪自强，俞法明.不同收获期春大豆种子贮藏后的活力研究[J].大豆科学，2000，19(1)：31 34.（Wang Z Q,Yu F M.Study on seed vigor of spring soybean in various ripening stage after four year low temperature storage[J].Soybean Science,2000,19(1):3134.）
相似文献/References:
[1]王宏光,孙殿君,马忠强,等.大豆根瘤菌HD001的分离鉴定及结瘤能力检测[J].大豆科学,2014,33(03):379.[doi:10.11861/j.issn.1000-9841.2014.03.0379]
WANG Hong-guang,SUN Dian-jun,MA Zhong-qiang,et al.Isolation and Identification of Rhizobium HD001 and Its Nodulation Capacity Test in Soybean Germplasm[J].Soybean Science,2014,33(01):379.[doi:10.11861/j.issn.1000-9841.2014.03.0379]
[2]郭亚辉,许志刚,杨光.大豆品种对大豆细菌性斑疹病的抗性[J].大豆科学,2011,30(02):263.[doi:10.11861/j.issn.1000-9841.2011.02.0263]
GUO Ya-hui,XU Zhi-gang,YANG guang.Resistance of Soybean Varieties to Bacterial Pustule Spot[J].Soybean Science,2011,30(01):263.[doi:10.11861/j.issn.1000-9841.2011.02.0263]
[3]郭泰,刘忠堂,王志新,等.优良大豆种质合丰35的利用与效果分析[J].大豆科学,2007,26(06):902.[doi:10.3969/j.issn.1000-9841.2007.06.018]
GUO Tai,LIU Zhong-tang,WANG Zhi-xin,et al.UTILIZATION OF ELITE SOYBEAN GERMPLASM HEFENG 35[J].Soybean Science,2007,26(01):902.[doi:10.3969/j.issn.1000-9841.2007.06.018]
[4]周蓉,王贤智,张晓娟,等.大豆种质倒伏抗性评价方法研究[J].大豆科学,2007,26(04):484.[doi:10.3969/j.issn.1000-9841.2007.04.007]
ZHOU Rong,WANG Xian-zhi,ZHANG Xiao-juan,et al.EVALUATION METHED OF LODGING RESISTANCE IN SOYBEAN GERMPLASM[J].Soybean Science,2007,26(01):484.[doi:10.3969/j.issn.1000-9841.2007.04.007]
[5]高赛男,赵雪,李文滨,等.农家大豆种质对花叶病毒病N1和N3株系的抗性鉴定[J].大豆科学,2016,35(01):117.[doi:10.11861/j.issn.1000-9841.2016.01.0117]
GAO Sai-nan,ZHAO Xue,LI Wen-bin,et al.Identification of Resistance of Soybean Landraces to N1 and N3 Strains of Soybean Mosaic Virus[J].Soybean Science,2016,35(01):117.[doi:10.11861/j.issn.1000-9841.2016.01.0117]
[6]徐巧珍 张学江 江木兰 李志玉.不同类型大豆种质资源共生固氮特性的鉴定与评价[J].大豆科学,1997,16(03):210.[doi:10.11861/j.issn.1000-9841.1997.03.0210]
Xu QiaozhenZha ng Xuejia ngJiang MulanLi Zhiyu.IDENTIFICATION AND EVALUATION OF GERMPLASMS FROMDIFFERENT SOYBEAN TYPES FOR SYMBIOTIC N2-FIXATION CHARACTERS[J].Soybean Science,1997,16(01):210.[doi:10.11861/j.issn.1000-9841.1997.03.0210]
[7]杨德,盖钧镒,马育华.我国南方大豆地方品种农艺和品质性状的遗传参数分析[J].大豆科学,1990,9(01):9.[doi:10.11861/j.issn.1000-9841.1990.01.0009]
[J].Soybean Science,1990,9(01):9.[doi:10.11861/j.issn.1000-9841.1990.01.0009]
[8]刘维志,刘晔,段玉玺.黄、淮、海地区大豆种质资源对大豆孢囊线虫1号生理小种的抗性鉴定研究[J].大豆科学,1991,10(04):327.[doi:10.11861/j.issn.1000-9841.1991.04.0327]
[J].Soybean Science,1991,10(01):327.[doi:10.11861/j.issn.1000-9841.1991.04.0327]
备注/Memo
基金项目：国家自然科学基金（31071436）；转基因生物新品种培育重大专项（2009ZX08009 088B）。
第一作者简介：高华伟（1988~），男，在读硕士,主要从事植物分子遗传学研究。E.mail:gaohuawei1025@126.com。
刘立科(1974~)，男，博士，副教授，主要从事植物分子遗传学研究。E.mail:liulike@lcu.edu.cn。