[1]程伟,许媛王自力,孔杰杰,等.江淮大豆育种种质对细菌性斑点病S1菌株的抗性鉴定[J].大豆科学,2016,35(01):100-105.[doi:10.11861/j.issn.1000-9841.2016.01.0100]
　CHENG Wei,XU Yuan,WANG Zi-li,et al.Evaluation of Resistance to the S1 Strain of Pseudomonas syringae pv. glycinea in a Soybean Breeding Line Population from Yangtze and Huaihe River Valleys[J].Soybean Science,2016,35(01):100-105.[doi:10.11861/j.issn.1000-9841.2016.01.0100]
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江淮大豆育种种质对细菌性斑点病S1菌株的抗性鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年01期 页码: 100-105 栏目: 出版日期: 2016-01-20
Title:
Evaluation of Resistance to the S1 Strain of Pseudomonas syringae pv. glycinea in a Soybean Breeding Line Population from Yangtze and Huaihe River Valleys
作者:
程伟1; 许媛2王自力1; 孔杰杰1; 高学文2; 盖钧镒1; 赵团结1
1.南京农业大学 大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室（综合）/作物遗传与种质创新国家重点实验室，江苏 南京 210095；
2.南京农业大学 植物保护学院，江苏 南京 210095
Author(s):
CHENG Wei1;  XU Yuan2;  WANG Zi-li1;  KONG Jie-jie1;  GAO Xue-wen2;  GAI Jun-yi;  ZHAO Tuan-jie1
1.Soybean Research Institute /National Center for Soybean Improvement/ Key Laboratory of Biology and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement, Ministry of Agriculture /National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;?
2. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
关键词:
大豆; 新品系; 大豆细菌性斑点病; 抗性鉴定; 相关分析
Keywords:
Soybean; Breeding line;  Pseudomonas syringae pv. glycinea; Resistance evaluation;  Correlation analysis
DOI:
10.11861/j.issn.1000-9841.2016.01.0100
文献标志码:
A
摘要:
大豆细菌性斑点病是江淮地区大豆生产中常见病害，但大豆种质资源抗性水平及抗源鉴定工作较少。本研究采用对大豆叶片正反面高压喷雾的接种方法鉴定了江淮地区309份育成品种（系）及亲本材料对大豆细菌性斑点病生理小种S1的抗感反应。结果表明：供试材料抗性差异明显，分别鉴定出高抗和中抗材料61和68份，占总数的19.74%和22.1%，表现为感病和高感的材料共有180份，占总数的58.25%。适合淮北和淮南地区种植的140和169份品种（系）中，抗病材料（高抗+中抗）分别有68和61份，感病材料（感病+高感）分别有72和108份，江淮淮北地区抗病品种（系）的比例高于淮南地区。供试材料抗性反应等级与成熟期等性状存在相关性。同时还发掘出徐豆18、南农99.6等高抗品种，及具有高蛋白、高油特性的优质抗性种质材料。
Abstract:
Bacterial leaf blight is one of the common diseases in soybean production in the Yangtze and Huaihe River Valleys. However, the resistance performance of soybean germplasm in this region remains unclear. In the present study, the resistance levels of 309 selected soybean varieties or breeding lines to Pseudomonas syringae pv-glycinea S1 strain were evaluated by inoculating both sides of leaves through spray method in field condition.The results showed that there were significant resistance differences in the sample 61 (19.74%) and 68 (22.1%) varieties or breeding lines were identified with high and medium resistance levels respectively, a total of 180(58.25%) varieties(lines) of the sample were found with susceptible or highly susceptible level. In the sample, 68 and 72 varieties(lines) were identified as resistant and susceptible in northern Huaihe River region respectively, and 72 and 108 varieties(lines) were identified as resistant and susceptible types in southern Huaihe River region respectively. The ratio of resistance varieties(lines) to Pseudomonas syringae pv. glycinea from Northern Huaihe River regions was higher than that form the southern region, and significant correlation between the disease resistance grade and maturity was found. Some high-resistant varieties(lines) including Xudou 18 and Nannnong 99.6 and some high-resistant lines with high seed protein content or oil content were identified and could be used in the resistance breeding and prevention of Pseudomonas syringaepv.glycinea in soybean

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备注/Memo

基金项目：国家高技术研究发展计划（“863计划”）（2012AA101106）；国家自然科学基金（31271750）；国家公益性行业（农业）科研专项经费（201203026-4）；长江学者和创新团队发展计划（PCSIRT13073）；江苏省优势学科建设工程专项（PAPD）；江苏省现代作物生产协同创新中心项目（JCIC-MCP）；转基因生物新品种培育重大专项（2014ZX08004001-010）。

第一作者简介：程伟（1988-），男，硕士，主要从事大豆抗病研究。E-mail：chengwei124@sina.cn。 通讯作者：赵团结（1969-），男，教授，博导，主要从事大豆遗传育种研究。E-mail: tjzhao@njau.edu.cn。

更新日期/Last Update: 2016-01-29