TAO Bo,WANG Huan,GAO Shi-jie.Study on Degradation Characteristics of Composite Fungi to Degrading Herbicide Chlorimuron-ethyl[J].Soybean Science,2015,34(05):855-860.[doi:10.11861/j.issn.1000-9841.2015.05.0855]
复合菌对大豆田土壤中氯嘧磺隆降解的研究
- Title:
- Study on Degradation Characteristics of Composite Fungi to Degrading Herbicide Chlorimuron-ethyl
- Keywords:
- Chlorimuron-ethyl; Soybean; HPLC; Microbial degradation; Composite multivariate
- 文献标志码:
- A
- 摘要:
- 采用高效液相色谱法,系统研究了不同降解菌及其多元混配在土壤中对氯嘧磺隆的降解作用。结果表明:真菌黑曲霉、青霉、F8和F31酿酒酵母在土壤中对氯嘧磺隆的降解速度存在显著差异,而且均能加快氯嘧磺隆的降解速度,其降解能力黑曲霉>F31>青霉>F8。黑曲霉分别和青霉、F8、F31混合后,各处理间差异显著,并且随着黑曲霉比例的增加,对土壤中除草剂的降解能力明显增强,其中F31和黑曲霉混合后效果最佳,降解率可达86.43%。对降解菌进行三元混配后发现,在二元复合的基础上添加少量青霉可进一步提高降解能力。
- Abstract:
- In this paper, a high performance liquid chromatography method was established for determination of the degradation rates of chlorimuron-ethyl.The biodegradation of herbicide chlorimuron-ethyl in the soil by different strains was researched systematically.The results showed that the degradation rates of herbicide chlorimuron-ethyl by Aspergillus niger, Penicillium, F8 and F31 were significantly different in the soil.The herbicide chlorimuron-ethyl could bedegraded effectively by all these four strains.The degradation ability was Aspergillus niger>F31> Penicillium>F8.The degradation rates of herbicide chlorimuron-ethyl by Aspergillus niger respectively mixed with Penicillium, F8 amd F31 were significantly different With the increase of proportion of Aspergillus niger, herbicide degradation ability was significantly enhanced. The F31 and Aspergillus nige blend was the best combination and the degradation rate reached 86.43%. On the basis of binary mixture, addition of a small amount of Penicillium could further improve the ability of degradation.
参考文献/References:
[1]邹月利,陶波.除草剂降解真菌对氯嘧磺隆的降解作用[J]土壤通报,2013(6):1445-1448(Zou Y L,Tao B. Research on the effects of different strains on the degradation of herbicide chlorimuron-ethyl[J].Chinese Journal of Soil Science,2013(6):1445-1448.)
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983~2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(05):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(05):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(05):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(05):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(05):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(05):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(05):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(05):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(05):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(05):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]宋艳宇,张浩,王岩,等.氯嘧磺隆在大豆植株和土壤中的残留动态研究[J].大豆科学,2007,26(04):634.[doi:10.3969/j.issn.1000-9841.2007.04.036]
SONG Yan-yu,ZHANG Hao,WANG Yan,et al.THE RESIDUE DYNAMIC OF CHLORIMURON-ETHYL IN SOYBEAN AND SOIL[J].Soybean Science,2007,26(05):634.[doi:10.3969/j.issn.1000-9841.2007.04.036]
备注/Memo
基金项目:国家自然科学基金(30971834)。