[1]郭娜,崔晓霞,赵晋铭,等.大豆疫霉根腐病相关miRNA的鉴定[J].大豆科学,2015,34(04):666-670.[doi:10.11861/j.issn.1000-9841.2015.04.0666]
　GUO Na,CUI Xiao-xia,ZHAO Jin-ming,et al.Identification of miRNA Resistant to Phytophthora Root Rot in Soybean[J].Soybean Science,2015,34(04):666-670.[doi:10.11861/j.issn.1000-9841.2015.04.0666]
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大豆疫霉根腐病相关miRNA的鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年04期 页码: 666-670 栏目: 出版日期: 2015-08-25
Title:
Identification of miRNA Resistant to Phytophthora Root Rot in Soybean
作者:
郭娜1; 崔晓霞1; 赵晋铭1; 闫强2; 武晓玲1; 窦道龙2; 邢邯1
1.南京农业大学 大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室/作物遗传与种质创新国家重点实验室，江苏 南京 210095；
?2.南京农业大学 植物保护学院，江苏 南京 210095
Author(s):
GUO Na1;  CUI Xiao-xia1;  ZHAO Jin-ming1; YAN Qiang2;  WU Xiao-ling1;  DOU Dao-long2;  XING Han1
1.Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China; 2.Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China
关键词:
栽培大豆; 保守miRNA; Northern blotting; 大豆疫霉根腐病
Keywords:
Glycine max; Conserved miRNAs; Northern blotting; Phytophthora root and stem rot
DOI:
10.11861/j.issn.1000-9841.2015.04.0666
文献标志码:
A
摘要:
microRNA (miRNA)是近年来在真核生物中发现的一类具有调控功能的非编码RNA，通过碱基互补在基因转录和转录后水平调控靶基因的表达，参与调控植物生长发育，并在响应多种生物及非生物胁迫中发挥重要作用。本文利用大豆疫霉菌2号生理小种（P6497）接种8个栽培大豆品种，通过Northern blotting技术，对大豆中的7个保守miRNAs进行检测。结果发现，有6个miRNAs在这8个大豆栽培品种中都有表达，但在接种病原菌前后没有明显的差异。使用高通量的小分子RNA芯片技术检测差异表达的miRNAs。利用抗性品种Williams 82和感病品种Williams分别接种P6497，获得部分与大豆疫霉菌侵染相关的miRNAs。结果显示，部分miRNAs可能参与调控大豆疫霉根腐病抗性。
Abstract:
The microRNAs (miRNAs), a type of short (20-24 nucleotides), non-coding RNA molecules, mediate repressive gene expression through RNA silencing at the transcriptional and post-transcriptional level, and play an important role in the regulation of plant growth and development, and defense and response to various abiotic and biotic stresses.Phytophthor a root and stem rot (PRR) of soybean caused by Phytophthora sojae is a destructive disease affecting soybean production regions throughout the world.However, the miRNAs-regulation networks and function in response to PRR are not clear in Glycine max. In this paper, seven conserved miRNAs were detected in eight cultivars of pre and post-inoculation by Northern blotting.The results showed that, six conserved miRNAs were expressed in eight cultivars in pre and post-inoculation, but there is no significant difference in pre-and post-inoculation Furthermore, we used microarray to detect miRNA expression pattern. The result showed that some miRNAs play very important roles in response to PRR in Glycin e max.

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备注/Memo

基金项目：南京农业大学青年科技创新基金（KJ2012001）；国家自然科学基金（31301340）；江苏省自然科学基金（BK20130684）；长江学者和创新团队发展计划（PCSIRT13073）。

第一作者简介：郭娜（1984-），女，博士，讲师，主要从事大豆抗病性研究。E-mail：guona@njau.edu.cn。
通讯作者：邢邯（1963-），男，教授，博导，主要从事大豆遗传和育种工作。E-mail：hanx@njau.edu.cn。

更新日期/Last Update: 2015-09-01