[1]孙晓环,潘相文,王燕平,等.大豆与南瓜远缘嫁接接合部组织形态解剖学研究[J].大豆科学,2015,34(04):639-642.[doi:10.11861/j.issn.1000-9841.2015.04.0639]
　SUN Xiao-huan,PAN Xiang-wen,WANG Yan-ping,et al.Anatomical Study of Integration Sections for Distant Grafting between Soybean and Pumpkin[J].Soybean Science,2015,34(04):639-642.[doi:10.11861/j.issn.1000-9841.2015.04.0639]
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大豆与南瓜远缘嫁接接合部组织形态解剖学研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年04期 页码: 639-642 栏目: 出版日期: 2015-08-25
Title:
Anatomical Study of Integration Sections for Distant Grafting between Soybean and Pumpkin
作者:
孙晓环1; 潘相文2; 3; 王燕平1; 白艳凤1; 宗春美1; 齐玉鑫1; 张彦丽4; 任海祥1
1.黑龙江省农业科学院 牡丹江分院，黑龙江 牡丹江 157041；?
2.黑龙江省农业科学院 博士后科研工作站，黑龙江 牡丹江 157041；?
3.中国科学院 东北地理与农业生态研究所/大豆分子设计育种重点实验室，黑龙江 哈尔滨 150081；
?4.牡丹江师范学院 生物系，黑龙江 牡丹江 157012
Author(s):
SUN Xiao-huan1;  PAN Xiang-wen2; 3 ;  WANG Yan-ping1;  BAI Yan-feng1;  ZONG Chun-mei1;  QI Yu-xin1;  ZHANG Yan-li5;  REN Hai-xiang1
1. Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157041, China;?
2. Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Mudanjiang 157041, China;
3. Key Laboratory of Molecular Design Breeding of Soybean, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China;?
4. Biology Department, Mudanjiang Normal University, Mudanjiang 157012, China
关键词:
大豆; 远缘嫁接; 接合部组织; 石蜡切片; 解剖结构
Keywords:
Soybean;  Distant grafting;  Integration sections;  Paraffin sections;  Anatomical structure
DOI:
10.11861/j.issn.1000-9841.2015.04.0639
文献标志码:
A
摘要:
以大豆品种黑农48为接穗，黑籽南瓜为砧木进行嫁接，接穗切面长度设置了5个不同处理。通过嫁接部位组织解剖学分析，深入研究接穗切面长度对嫁接成活率的影响及组织变化规律，探索嫁接诱导植物变异的机理。结果表明：各处理间成活率差异显著，接穗切面长度为1.0 cm时成活率最高，达到80%，愈合的速度也最快；其次是1.3 cm。对1.0 cm接穗切面结合部不同时期的电镜动态观察表明，接穗与砧木的愈合过程可分为三个阶段：愈伤组织形成期（1～8 d）、维管组织分化期（9～12 d）、维管组织分化完成期（13～35 d）。研究结果可为大豆远缘嫁接提供理论和实践参考。
Abstract:
Dynamic changes of morphological anatomy of integration sections for distant grafting between soybean and pumpkin were analyzed to explore the mechanism of graft-induced plant variation.Soybean scions from soybean varieties Heinong 48 were grafted onto the stocks (black seed pumpkin) with five treatments for the section length of soybean scions so that the effects of scion section length on graft survival rate and the dynamic changes of morphological anatomy of integration sections were explored.The results showed that there were significant differences for the survival rate between the groups, the highest survival rate could reach 80% with the fastest healing speed when the scion section length was 1.0 cm, and 1.3 cm scion section length ranked the second. Electron microscopy scan results of integration sections for 1.0 cm scion section length at different periods after grafting showed that the healing process of scion and stock could be divided into three stages: Callus formation stage (1.8 d after grafting); Vascular tissue differentiation stage (9.12 d after grafting); Terminal stage of vascular tissue differentiation (13.35 d after grafting). Those results would provide theoretical and practical reference for soybean distant grafting.

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备注/Memo

基金项目：黑龙江省农业科技创新工程院级科研项目（2012QN013）。

第一作者简介：孙晓环(1983-)，女，硕士，助理研究员，主要从事大豆遗传育种研究。E-mail:xaozm@yeah.net。
通讯作者：潘相文（1972-），男，博士，高级工程师，主要从事大豆远缘嫁接与分子育种研究。E-mail:panxw52579@163.com。

更新日期/Last Update: 2015-09-01