[1]邓莹莹,赵双进,闫龙,等.定向选择对大豆ms1轮回群体遗传基础的影响[J].大豆科学,2015,34(04):548-554.[doi:10.11861/j.issn.1000-9841.2015.04.0548]
　DENG Ying-ying,ZHAO Shuang-jin,YAN Long,et al.Effect of Orthoselection on Agronomic Traits and Genetic Base of a Male Sterile Soybean Recurrent Population[J].Soybean Science,2015,34(04):548-554.[doi:10.11861/j.issn.1000-9841.2015.04.0548]
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定向选择对大豆ms1轮回群体遗传基础的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年04期 页码: 548-554 栏目: 出版日期: 2015-08-25
Title:
Effect of Orthoselection on Agronomic Traits and Genetic Base of a Male Sterile Soybean Recurrent Population
作者:
邓莹莹1; 赵双进2; 闫龙2; 杨春燕1; 刘兵强2; 邸锐2; 东方阳1; 张孟臣1; 2
1河北科技师范学院 生命科技学院，河北 昌黎 066600；
2.河北省农林科学院 粮油作物研究所/国家大豆改良中心石家庄分中心/农业部黄淮海大豆生物学与遗传育种重点实验室/河北省作物遗传育种重点实验室，河北 石家庄 050031
Author(s):
DENG Ying-ying1; ZHAO Shuang-jin2; YAN Long2; YANG Chun-yan1; LIU Bing-qiang2; DI Rui2; DONGFANG Yang1; ZHANG Meng-chen1; 2
1College of Biological Science and Technology，Hebei Normal University of Science Technology,Changli 066600,China;2.Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences/Shijiazhuang Branch of National Soybean Improvement Center/Huanghuaihai Key Laboratory of Biology and Genetic Improvement of Soybean,Ministry of Agriculture,Shijiazhuang 050035, China
关键词:
大豆; 轮回群体; 雄性不育; 定向选择; 遗传多样性
Keywords:
Soybean;  Recurrent population;  Male sterile;  Selection;  Genetic diversity
DOI:
10.11861/j.issn.1000-9841.2015.04.0548
文献标志码:
A
摘要:
在分析ms1轮回群体遗传多样性和群体内材料分组关系的基础上，探讨了定向选择对群体遗传基础的影响。从ms1轮回群体中随机抽取216份材料为原始群体，利用38个均匀分布于大豆全基因组的SSR标记，检测原始群体等位变异数目和遗传多样性指数。并分别根据成熟期、株高和百粒重对群体定向选择形成6个不同类型的定向选择群体，比较选择前后群体遗传多样性变化。结果表明：在原始群体中，共检测出216个等位变异，平均每个位点等位变异数5.68，平均多样性指数PIC为0.71。对原始群体进行熟期、株高和百粒重定向选择后，各定向选择群体平均每个位点等位变异数介于4.89~5.13，平均多样性指数PIC介于0.67~0.71。定向选择群体中有16个位点的等位变异频率发生了变化。对大豆ms1轮回群体定向改良，目标性状及相关农艺性状的表现值及群体分布频率随之发生改变，对群体遗传结构和遗传多样性指数的影响显著小于对目标性状及相关农艺性状的影响。
Abstract:
The use of male sterile 1 (ms1) in a recurrent selection scheme can facilitate the soybean ［Glycine max (L) Merr］breeding process and improve breeding efficiency.In this study, the effect of artificial selection on agronomic traits, genetic diversity, and genetic structure was analyzed using an ms1 recurrent population.Two hundred and sixteen individuals from a recurrent soybean population were used as a base population, and six improved populations derived from the base population were defined, based on maturity (early vs late-maturing), plant height (tall vs- short), and seed size (large vs small). Genetic diversity was assessed by allele number and polymorphism information content (PIC) using 38 simple sequence repeat (SSR) loci.The base population was compared with the improved populations to identify changes in agronomic traits and genetic diversity. Significant differences were found in growth duration, plant height, and 100-seed weight in different improved populations. A total of 216 alleles were detected in the base population, with an average of 5.68 and a mean PIC of 0.71 per SSR locus, while the total allele numbers ranged from 186 to 195 in the improved populations, with the average ranging from 4.89 to 5.13 and the mean PIC ranging from 0.67 to 0.71 per SSR locus.The allele frequencies of 16 loci varied among different populations. Improving the ms1 soybean recurrent groups, performance value of target traits and agronomic traits and population distribution of frequency changed as well, the impact of population genetic structure and genetic diversity index was significantly less than the target properties and agronomic traits.

参考文献/References:

［1］盖钧镒,赵团结,崔长林,等.中国1923-1995年育成的651个大豆品种的遗传基础［J］中国油料作物学报, 1998, 20(1)：17-23（Gai J Y, Zhao T J, Cui C L, et al.The genetic base for 651 soybean cultivars released during 1923-1995 in China［J］Chinese Journal of Oil Crop Sciences, 1998, 20(1)：17-23）

［2］Tanksley S D, McCouch S R. Seed banks and molecular maps:Unlocking genetic potential from the wild［J］.Science, 1997,227(22):1063-1066
［3］Hyten L, Song Q J, Zhu Y L. Impacts of genetic bottlenecks on soybean genome diversity［J］. PNAS, 2006, 103:16666-16671
［4］盖钧镒,赵团结.中国大豆育种的核心祖先亲本分析［J］.南京农业大学学报, 2001, 24 (2):20-23（Gai J Y, Zhao T J. The core ancestors of soybean cultivars in China［J］.Journal of Nanjing Agricultural University, 2001, 24 (2):20-23）
［5］Gizlice Ziya, Carter T E J R, Burton J W. Genetic base for north American public soybean cultivars released between 1947-1988［J］.Crop Science, 1994, 34: 1143-1551
［6］Colkert T R, Darrah L L, Zuber M S.Effect of recurrent selection for stalk crushing strength on agronomic characteristics and soluble solids in maize［J］.Crop Science, 1984, 24: 473-478
［7］高庆荣,孙兰珍,刘保申,等. K、V 型小麦雄性不育恢复系轮回选择的研究Ⅰ轮选群体的育性分离及性状表现［J］. 麦类作物学报, 2001, 21(1):21-24（Gao Q R, Sun L Z, Liu B S, et al.Study on recurrent selection for breeding restorer of K,V.cytoplasm male-sterility in wheat I. Performance of the fertility and its characters in recurrent selection population［J］ Journal of Triticeae Crops, 2001, 21 (1):21-24）
［8］吴长明,傅秀林,金京花.粳稻轮回选择育种方法的研究［J］. 吉林农业科学, 1994, 4(3):14-18（Wu C M, Fu X L, Jin J H. Study on breeding method of recurrent selection in japonica- rice［J］Jilin Agricultural Sciences, 1994, 4(3):14-18）
［9］Kenworthy W J, Brim C A Recurrent selection in soybeans I Seed yield［J］ Crop Science, 1979, 19: 315-318
［10］Miller J E, Fehr W R.Direct and indirect recurrent selection for protein in soybeans［J］ Crop Science, 1979, 19: 101-106
［11］Burton J W, Brim C A. Recurrent selection in soybeans Ⅲ Selection for increased percent oil in seeds［J］.Crop Science, 1981, 21: 31-34
［12］盖钧镒, Fehr W R.大豆产量轮回选择主要农艺性状的遗传响应［J］. 作物学报, 1985, 11(4):236-242（Gai J Y, Fehr W R. Genetic effects of main agronomy characteristics of soybean yield recurrent selection［J］Acta Agronomica Sinica, 1985, 11(4):236-242）
［13］宋启建,吴天侠,盖钧镒.熟期选择对大豆轮回群体质量、数量性状及不育率的影响［J］.中国农业科学, 1996, 29(3):49-54（Song Q J, Wu T X, Gai J Y. Effect of maturity selection in a soybean recurrent population on morphological and agronomic traits and ratio of male-sterile plants［J］. Scientia Agricultura Sinica, 1996, 29 (3):49-54）
［14］赵双进,张孟臣,蒋春志,等.大豆ms1轮回群体品质改良效应与分离特性研究［J］中国农业科学, 2006, 39(12):2422-2427.（Zhao S J, Zhang M C, Jiang C Z,et al. Study on quality improvement effect and separate character of soybean male sterile (ms1) recurrent selection population［J］Scientia Agricultura Sinica, 2006, 39(12):2422-2427）
［15］Song Q J, Marek L F, Shoemaker R C. A new integrated genetic linkage map of the soybean［J］. Theoritical and Applied Genetics, 2004, 109: 122-128
［16］盖钧镒. 植物种质群体遗传结构改变的测度［J］植物遗传资源学报, 2005, 6(1):1-8（Gai J Y. Indicators related to genetic structure changes of plant qermplasm population［J］Journal of Plant Genetic, 2005, 6(1):1-8）
［17］李英慧,刘燕,关荣霞 “十五”大豆创新种质和1963-1995年间育成品种的SSR遗传结构及遗传多样性分析［J］作物学报, 2007, 33 (10):1630-1636 (Genetic structure and diversity of both Enhanced germplasms developed during 10th fiveyear plan and modern cultivars released during 1963-1995 in China［J］.Acta Agronomica Sinica, 2007, 33 (10):1630-1636)
［18］杨春燕,闫龙,张孟臣.河北省大豆地方品种遗传基础［J］.植物遗传资源学报, 2009, 10(4):560-565 (Yang C Y, Yan L, Zhang M C. Genetic basic of landrace soybean in Hebei［J］ Journal of Plant Genetic, 2009, 10(4):560-565)
［19］黄素华,滕文涛,王玉娟,等.利用SSR标记分析玉米轮回选择群体的遗传多样性［J］遗传学报, 2004, 31(1):73-80 (Huang S H, Teng W T, Wang Y J,et al.Genetic diversity analysis of maize recurrent selection populations by SSR marker［J］. Acta Genetica Sinica, 2004, 31(1):73-80)
［20］Sumarno,Fehr W R.Response to recurrent selection for yield in soybeans［J］Crop Science, 1982,22(2):295-299
［21］朱成松,顾和平,盖钧镒.大豆产量轮回选择的初步研究［J］江苏农业科学, 1998(2):80-84(Zhu C S, Gu H P, Gai J Y. A Preliminary study on recurrent selection for yield in soybeans［J］.Jiangsu Agricultural Sciences,1998, 14(2):80-84)
［22］Lee S H, Bailey M A, Mian M A R, et al. Molecular markers associated with soybean plant height, lodging, and maturity across locations［J］.Crop Science,1996,36: 728-734
［23］Sebolt A M, Shoemaker R C, et al.Analysis of a quantitative trait locus allele from wild soybean that increases seed protein concentration in soybean［J］Crop Science, 2000, 40: 1438-1444
［24］Wang D G, Graef G L, Procopiuk A M, et al.Identification of putative QTL that underlie yield in interspecific soybean backcross populations［J］. Theoretical and Applied Genetics, 2004, 108: 458-467
［25］Specht J E, Chase K, Macrander M, et al. Soybean response to water: A QTL analysis of drought tolerance［J］. Crop Science, 2001, 41: 493-509
［26］Orf J H, Chase K, Jarvik T, et al.Genetics of soybean agronomic traits: I. Comparison of three related recombinant inbred populations［J］. Crop Science, 1999, 39: 1642-1651
［27］Maughan P J, Maroof M A S, Buss G R, et al.Molecular-marker analysis of seed-weight: genomic locations, gene action, and evidence for orthologous evolution among three legume species［J］. Theoretical Applied Genetics, 1996, 93: 574-579
［28］Hyten D L, Pantalone V R, Sams C E, et al.Seed quality QTL in a prominent soybean population［J］. Theoretical and Applied Genetics, 2004,109:552-561
［29］Panthee D R, Pantalone V R, West D R, et al. Quantitative trait loci for seed protein and oil concentration, and seed size in soybean［J］ Crop Scienc,2005,45(5):2015-2022.

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备注/Memo

基金项目：国家现代农业产业技术体系（CARS-004-PS06）；国家高技术发展研究计划“863计划”（2012AA101106）；国家“十二五”科技支撑计划(2011BAD35B06)；国家转基因生物新品种培育重大专项（2013ZX08004-001）；河北省科技支撑计划（14226309D）。

第一作者简介：邓莹莹（1985-），女，硕士，主要从事大豆分子数量遗传与育种研究。E-mail:dengyingyingyy@126.com。
通讯作者：张孟臣（1956-），男，研究员，主要从事大豆遗传育种研究。E-mail:mengchenzhang@hotmail.com。

更新日期/Last Update: 2015-08-31