[1]舒英杰,周玉丽,时侠清,等.大豆种子发芽期耐旱性鉴定的适宜PEG-6000浓度筛选[J].大豆科学,2015,34(01):56-59.[doi:10.11861/j.issn.1000-9841.2015.01.0056]
　SHU Ying-jie,ZHOU Yu-li,SHI Xia-qing,et al.Screening of Appropriate PEG-6000 Concentration for the Identification of Soybean Drought Tolerance at Germination Stage[J].Soybean Science,2015,34(01):56-59.[doi:10.11861/j.issn.1000-9841.2015.01.0056]
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大豆种子发芽期耐旱性鉴定的适宜PEG-6000浓度筛选

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年01期 页码: 56-59 栏目: 出版日期: 2015-02-25
Title:
Screening of Appropriate PEG-6000 Concentration for the Identification of Soybean Drought Tolerance at Germination Stage
作者:
舒英杰; 周玉丽; 时侠清; 胡能兵; 邵庆勤; 杜军利
安徽科技学院 农学院，安徽 凤阳 233100
Author(s):
SHU Ying-jie;  ZHOU Yu-li; SHI Xia-qing; HU Neng-bing; SHAO Qing-qin; DU Jun-li
Agricultural College of Anhui Science and Technology University, Fengyang 233100, China
关键词:
大豆; PEG-6000; 干旱胁迫; 发芽期; 耐旱性
Keywords:
Soybean(Glycine max);  Polyethylene glycol (PEG-6000);  Drought stress; Germination stage; Drought tolerance
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.01.0056
文献标志码:
A
摘要:
以抗旱性不同的3个大豆品种（湘豆3号、Lee68和宁镇1号）为试材，研究了不同浓度（5%、10%、15%、20%、25%和30%）的PEG-6000模拟发芽期干旱胁迫对大豆种子发芽、子叶苗膜脂过氧化以及渗透调节物质含量的影响。结果表明：10%以下的PEG-6000能起引发作用，促进大豆种子的萌发，显著提高发芽势和发芽率；随着PEG-6000浓度的升高，大豆种子的发芽明显受抑制，当PEG-6000浓度大于25%时抑制更明显，发芽势、发芽率、发芽指数和简易活力指数显著下降；随着PEG-6000浓度的升高，大豆子叶苗中的丙二醛（MDA）、脯氨酸（Pro）、可溶蛋白和可溶性糖含量显著增加，PEG-6000浓度在15%~25%间增加幅度最大，PEG-6000浓度小于15%或大于25%时增加不明显。综合种子发芽、子叶苗的膜脂过氧化程度以及渗透调节物质含量的变化，可初步确定室内采用PEG-6000浸种法模拟干旱胁迫鉴定大豆发芽期耐旱性的适宜浓度为20%~25%。
Abstract:
Three soybean varieties（Ningzhen 1，Lee68 and Xiangdou 3）were used to study the effect of different PEG-6000 concentrations (5%, 10%, 15%, 20%, 25% and 30%)on germination of soybean seed, membrane lipid peroxidation and osmotic substances of soybean seedlings. Results showed that when the PEG-6000 concentration less than 10%，which can promote soybean seeds germination, improve the germination potential and germination rate significantly. When the PEG-6000 concentrations were more than 25%, with the increasement of PEG-6000 concentration,germination potential, germination rate, germination index and simple vigor index of soybean seed decreased significantly.The contents of malondialdehyde(MDA), proline (Pro), soluble protein and soluble sugar increased significantly with the PEG-6000 concentration increased, especially when the PEG-6000 concentration was between 15% and 25%.We can conclude preliminarily that the appropriate PEG-6000 concentration for the identification of soybean drought tolerance in seed germination stage was 20%-25% by synthesizing seed germination, membrane lipid peroxidation and the content change of osmotic substances.

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备注/Memo

基金项目：安徽省教育厅高等学校省级优秀青年人才基金（2012SQRL143）；国家自然科学基金（31101212）。

第一作者简介：舒英杰（1977-），男，博士，副教授，主要从事种子学与大豆遗传育种研究。E-mail：shuyj@ahstu.edu.cn。

更新日期/Last Update: 2015-04-12