[1]魏思楠,朱晓峰,陈立杰,等.我国不同地区大豆主栽品种种子携带真菌研究[J].大豆科学,2014,33(06):890-895.[doi:10.11861/j.issn.1000-9841.2014.06.0890]
　WEI Si-nan,ZHU Xiao-feng,CHEN Li-jie,et al.Seed-Borne Fungi Detection in Different Soybean Cultivars[J].Soybean Science,2014,33(06):890-895.[doi:10.11861/j.issn.1000-9841.2014.06.0890]
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我国不同地区大豆主栽品种种子携带真菌研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年06期 页码: 890-895 栏目: 出版日期: 2014-12-25
Title:
Seed-Borne Fungi Detection in Different Soybean Cultivars
文章编号:
1000-9841.2014.06.0890
作者:
魏思楠; 朱晓峰; 陈立杰; 王媛媛; 段玉玺
沈阳农业大学，北方线虫研究所，辽宁 沈阳 110866
Author(s):
WEI Si-nan; ZHU Xiao-feng; CHEN Li-jie; WANG Yuan-yuan; DUAN Yu-xi
Shenyang Agricultural University,Nematology Institute of Northern China,Shenyang 110866,China
关键词:
大豆; 种子带菌; 种子质量检测
Keywords:
Soybean; Seed-borne fungi; Seed healthy testing
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.06.0890
文献标志码:
A
摘要:
采用组织分离法与形态鉴定相结合，对全国不同大豆产区的304个大豆主栽品种种子携带真菌情况进行了研究。结果表明：大豆种子携带的真菌主要有10个属，其中从大豆表面分离到的真菌中青霉属( Penicillium spp.)所占比例最大，其次是曲霉属( Aspergillus spp.)、镰刀属（Fusarium spp.）和根足霉属( Rhizopus spp.)，此外还有链格孢属(Alternaria spp.）、木霉属(Trichoderma spp.）、枝孢属(Cladosporium spp.)、尾孢属(Cercospora spp.）、赤霉属（Gibberella spp.）、疫霉属(Phytophthora spp.)；大豆种皮内携带的真菌优势种群为青霉属，其次为镰刀属，此外还有根足霉属、木霉属等；大豆种子内寄藏的真菌中青霉属占绝对优势，其次为镰刀属和曲霉属，此外还有链格孢属、根足霉属等。种外、种内携带的优势种群相互影响很大,呈正相关。
Abstract:
With the method combining the tissue separation culture and morphological identification,the seed-born fungi of the national 304 main cultivars of soybean from China was detected.The results showed that there were 10 genera of fungi were isolated from soybean seeds.The dominant population was Penicillium spp.,followed by Aspergillus spp.,Fusarium spp.and Rhizopus spp.In addition the other genera were also founded,such as Alternaria spp.,Trichoderma spp.,Cladosporium spp.,Cercospora spp.,Gibberella spp.,Rhizopus spp. and Phytophthora spp..The fungi was carried in the coat of soybean seed,the advantage fungi group was Penicillium spp.,the second was Fusrium spp.,then were Rhizopus spp.,Trichoderma spp. in addition.The fungi hiden in the inner of soybean seeds,Penicillium was dominant population,the second was Fusrium and Aspergillus,Alternaria and Rhizopus were in addition.The mutual influence was great between the dominant fungi population of different place in seed.They are positive correlation.

参考文献/References:

［1］宋培玲,李子钦.不同油菜品种种子带菌检测[J].中国油料作物学报,2011,33(2):193-196.(Song P L,Li Z Q.The bacteria detection of different cultivars oilseed rape seed[J].Chinese Journal of Oil Crop,2011,33(2):193-196.)

[2]方中达.植病研究方法[M].北京：农业出版社，1979.(Fang Z D.Method of plant pathology research[M].Beijing:Agricultural Press,1979.)
[3]刘西莉,李健强,朱春雨,等.种衣剂处理大豆种子的生物学效应[J].云南农业科技,2000(4):7-10.(Liu X L,Li J Q,Zhu C Y,et al.The biological effects of seed coating treatment on soybean seed[J].Yunnan Agricultural Science and Technology,2000(4):7-10.)
[4]刘西莉,李健强,朱春雨,等.不同水稻品种种子带菌检测及药剂消毒处理效果[J].中国农业大学学报,2000(5):42-47.(Liu X L,Li J Q,Zhu C Y,et al.Testing and chemical disinfection effect of different varieties rice seed[J].Journal of China Agricultural University,2000(5):42-47.)
[5]李健强,刘西莉,朱春雨,等.云南省玉米种子带菌检测及种衣处理的生物学效应[J].云南农业大学学报,2001,16(1):5.(Li J Q,Liu X L,Zhu C Y,et al.Yunnan province corn seed detection assays and biological effect garment processing[J].Journal of Yunnan Agricultural University,2001,16(1):5.)
[6]高晓梅,吕国忠.玉米种子携带真菌的多样性研究[J].菌物研究,2005(2):42-46.(Gao X M,Lyu G Z.The fungal diversity of fungi on corn seed[J].Fungal Research,2005(2):42-46.)
[7]孙君灵,宋晓轩,朱何琴.棉花枯、黄萎病种子带菌量的探讨[J].中国棉花,1998,25(6):11-12.(Sun J L,Song X X,Zhu H Q.Discussion of cotton blight and Verticillium wilt infected seed quantity[J].China Cotton,1998,25(6):11-12.)
[8]杨峻,刘西莉,王慧敏,等.五种豆科林草种子带菌检测及药剂消毒处理效果[J].种子,2002(1):20-21.(Yang J,Liu X L,Wang H M,et al.Detection bacteria and chemical disinfection effect of five kinds leguminous grass seed[J].Seed,2002(1):20-21.)
[9]Roy K W,Baird R E,Abney T S.A review of soybean(Glycine max)seed,pod,and flower mycofloras in North America,with methods and a key for identification of selected Fungi[J].Mycoputhologiu,2000,150:15-27.
[10]周肇蕙,严进.大豆疫病的种子检验技术[J].中国进出境动植检,1997（2）:30-31.（Zhou Z H,Yan J.Seed testing technology of soybean blight[J].China Entry and Exit Animal and Plant Quarantine,1997(2):30-31.）
[11]周肇蕙，严进.大豆疫病的检测研究[J].植物检疫,1996(5):257.(Zhou Z H,Yan J.Study on detection of Phytophthora- root rot of soybean[J].Plant Quarantine,1996(5):257.)

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备注/Memo

基金项目：国家现代农业产业技术体系岗位科学家专项（CARS-04-PS13）。

第一作者简介：魏思楠（1988-），女，硕士，主要从事植物病理学研究。E-mail:837575348@qq.com。

更新日期/Last Update: 2014-12-29