QIAO Ya-ke,YANG Xiao-qian,QIAO Xiao,et al.The Correlation of Drought-Resistance Evaluation between Agronomic Traits and Physiological Indexes of Wild and Cultivated Soybean[J].Soybean Science,2014,33(05):667-673.[doi:10.11861/j.issn.1000-9841.2014.05.0667]
大豆基于形态及生理指标的抗旱性评价及相关性分析
- Title:
- The Correlation of Drought-Resistance Evaluation between Agronomic Traits and Physiological Indexes of Wild and Cultivated Soybean
- 文章编号:
- 1000-9841.2014.05.0667
- Keywords:
- Soybean; Seedling stage; Flowering phase; Drought resistance; Agronomic traits; Physiological indexes; Correlation
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以野生、半野生和栽培大豆为供试材料,盆栽条件下于苗期和花期进行干旱胁迫处理,测定农艺性状及生理指标,利用隶属函数法对鉴定的指标进行综合分析,并对不同时期的形态和生理指标与抗旱性的相关性进行比较。结果表明:在苗期和花期干旱胁迫,不同类型大豆抗旱性鉴定结果表现基本相同。干旱胁迫下,形态指标与生理指标的综合隶属函数值呈极显著正相关,以二者评价大豆的抗旱性结果高度一致;形态指标中单株荚数、单株粒数和单株粒重与抗旱性的关系更为密切(r≥0.90);苗期和花期干旱胁迫大豆的生理反应不同,苗期与抗旱性关系密切的生理指标为可溶性糖含量和过氧化氢酶(CAT)活性(r=0.95),开花期与抗旱性关系密切的生理指标为可溶性糖含量和叶绿素含量(r≥0.92)。
- Abstract:
- Wild,semi-wild and cultivated soybeans were used as materials to study the evaluation system of drought resistance identification of soybean.The drought stress treatment was conducted at seedling and flowering stage under pot experiment condition.The method of subordinate function was used to comprehensive analyze the correlation of drought resistance and agronomic traits and physiological indexes.The result showed that the reaction of different types of soybeans were the same no matter in seedling or flowering stage.The comprehensive membership function values of morphological indexes and physiological indexes had significant positive correlation under the drought stress condition.The drought resistance results of soybeans were highly consistent assessed by the two indicator systems.Morphological indexes for pods per plant,plant grains and plant grain weight associated with drought resistance more closely among the agronomic traits.The physiological response of soybean to drought stress was different in seedling stage and flowering phase.The physiological indexes closely associated with drought resistance were the soluble sugar content and CAT activity in seedling stage,but it were the soluble sugar content and chlorophyll content in flowering phase.Soluble sugar content is the reliable physiological index of drought resistance of soybean.
参考文献/References:
[1]史宏,刘学义.野生大豆抗旱性鉴定及研究[J].大豆科学,2003,22(4):264-268.(Shi H,Liu X Y.Studies on the drought resistance of wild soybean germplasm[J].Soybean Science,2003,22(4):264-268.)
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983~2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(05):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(05):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(05):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(05):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(05):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(05):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(05):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(05):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(05):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(05):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]宋晓慧,滕占林,箫长亮,等.淹水胁迫对不同耐涝性大豆品种苗期根部形态及叶部生理指标的影响[J].大豆科学,2013,32(01):130.[doi:10.3969/j.issn.1000-9841.2013.01.030]
SONG Xiao-hui,TENG Zhan-lin,XIAO Chang-liang,et al.Effect of Waterlogging on Root Morphology and Foliar Physiological Indexes of Soybean Varieties[J].Soybean Science,2013,32(05):130.[doi:10.3969/j.issn.1000-9841.2013.01.030]
[12]宋晓慧,张智杰,李春光,等.淹水时间对不同耐涝性大豆品种苗期根部形态和叶部生理指标的影响[J].大豆科学,2014,33(01):70.[doi:10.11861/j.issn.1000-9841.2014.01.0070]
SONG Xiao-hui,ZHANG Zhi-jie,LI Chun-guang,et al.Effect of Waterlogging Time on Root Morphology and Foliar Physiological Indexes of Soybean Varieties[J].Soybean Science,2014,33(05):70.[doi:10.11861/j.issn.1000-9841.2014.01.0070]
[13]郝青南,王程,陈水莲,等.大豆苗期氮高效和氮敏感资源的筛选研究[J].大豆科学,2011,30(06):910.[doi:10.11861/j.issn.1000-9841.2011.06.0910]
HAO Qing-nan,WANG Cheng,CHEN Shui-lian,et al.Screening of Soybean Varieties with Different Nitrogen Efficiency at Seedling Stage[J].Soybean Science,2011,30(05):910.[doi:10.11861/j.issn.1000-9841.2011.06.0910]
[14]王 敏,杨万明,杜维俊.苗期大豆根系及地上部性状与耐旱性的关系[J].大豆科学,2012,31(03):399.[doi:10.3969/j.issn.1000-9841.2012.03.013]
WANG Min,YANG Wan-ming,DU Wei-jun.Root and Aboveground Characteristics at Seedling and Their Relationship with Drought Tolerance in Soybean[J].Soybean Science,2012,31(05):399.[doi:10.3969/j.issn.1000-9841.2012.03.013]
[15]臧紫薇,赵雪,李海燕,等.大豆种质资源苗期抗旱性评价[J].大豆科学,2016,35(06):964.[doi:10.11861/j.issn.1000-9841.2016.06.0964]
ZANG Zi-wei,ZHAO Xue,LI Hai-yan,et al.Evaluation of Drought Resistance of Soybean Germplasm in Seedling Stage[J].Soybean Science,2016,35(05):964.[doi:10.11861/j.issn.1000-9841.2016.06.0964]
[16]王应党,许孟歌,张雅娟,等.江淮大豆育种种质苗期耐旱性鉴定[J].大豆科学,2017,36(05):669.[doi:10.11861/j.issn.1000-9841.2017.05.0669]
WANG Ying-dang,XU Meng-ge,ZHANG Ya-juan,et al.Identification of Drought-tolerance of Soybean Germplasms from Yangtze and Huaihe River Valleys at Seedling Stage[J].Soybean Science,2017,36(05):669.[doi:10.11861/j.issn.1000-9841.2017.05.0669]
[17]牛远,杨修艳,戴存凤,等.大豆芽期和苗期耐盐性评价指标筛选[J].大豆科学,2018,37(02):215.[doi:10.11861/j.issn.1000-9841.2018.02.0215]
NIU Yuan,YANG Xiu-yan,DAI Cun-feng,et al.Related Indices Selection of Soybean Salt Tolerance at Germination and Seedling Stages[J].Soybean Science,2018,37(05):215.[doi:10.11861/j.issn.1000-9841.2018.02.0215]
[18]何松榆,秦彬,张明聪,等.水分胁迫下外源褪黑素对大豆苗期抗氧化特性和产量的影响[J].大豆科学,2019,38(03):407.[doi:10.11861/j.issn.1000-9841.2019.03.0407]
HE Song-yu,QIN Bin,ZHANG Ming-cong,et al.Effects of Exogenous Melatonin on Antioxidant Properties and Yield of Soybean Seedling Under Water Stress[J].Soybean Science,2019,38(05):407.[doi:10.11861/j.issn.1000-9841.2019.03.0407]
[19]曲芳,陈海涛,王洪飞,等.仿生包衣装置对大豆发芽与苗期植株性状的影响[J].大豆科学,2019,38(03):455.[doi:10.11861/j.issn.1000-9841.2019.03.0455]
QU Fang,CHEN Hai-tao,WANG Hong-fei,et al.Effects of Bionic Coating Device Treatment on Soybean Germination and Plant Characters at Seedling Stage[J].Soybean Science,2019,38(05):455.[doi:10.11861/j.issn.1000-9841.2019.03.0455]
[20]刘蓓,邱爽,何佳琦,等.8个大豆Dof转录因子的生物信息学分析及干旱诱导表达[J].大豆科学,2020,39(03):377.[doi:10.11861/j.issn.1000-9841.2020.03.0377]
LIU Bei,QIU Shuang,HE Jia-qi,et al.Bioinformatics Analysis and Expression of Eight Dof Transcription Factors in Soybean Under Drought Stress[J].Soybean Science,2020,39(05):377.[doi:10.11861/j.issn.1000-9841.2020.03.0377]
备注/Memo
基金项目:转基因生物新品种培育科技重大专项(20142X08004-004B);河北省科技支撑计划(11220107D);河北省自然科学基金(C2009000868)。