YANG Li-juan,GU Di-zhou,LUAN Zhi-hui,et al.Effect of Calcium and Lead Treatment on Mitosis of Soybean Root Tip Cell[J].Soybean Science,2014,33(02):293-295.[doi:10.11861/j.issn.1000-9841.2014.02.0293]
钙、铅处理对大豆根尖细胞有丝分裂的影响
- Title:
- Effect of Calcium and Lead Treatment on Mitosis of Soybean Root Tip Cell
- 文章编号:
- 1000-9841(2014)02-0293-03
- Keywords:
- Calcium and lead treatment; Soybean; Cell mitosis
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以大豆(2n=40)品种黑河26为材料,研究了铅、钙及二者复合处理对大豆根尖细胞有丝分裂的影响。结果表明:铅、钙及其复合处理对大豆根尖细胞有丝分裂及染色体行为存在不同程度的影响。具体表现为:在0~100 mg.L-1范围内,随Pb(NO3)2浓度增大染色体畸变率、微核率递增,有丝分裂指数则降低;0.1~1.0 g.L-1浓度Ca(NO3)2有利于植物根尖有丝分裂的进行,但大于2.5 g.L-1则具有抑制作用;铅钙复合处理条件下,钙在0.1~1.0 g.L-1浓度范围会缓解铅的毒害,钙的浓度过高反而会加重铅的毒性效应。
- Abstract:
- In this paper,we studied the effect of Pb,Ca and their combined treatments on root cell mitosis of soybean(2n=40)cultivar Heihe 26.The results showed that with the concentration of Pb(NO3)2 (0-100 mg.L-1)increased the rate of chromosome aberration and micronucleus rate increased,and the mitotic index decreased.Lower concentration of Ca(NO3)2(0.1-1.0 g.L-1)could enhance the plant root tip mitosis index,but inhibited that at high concentrations(2.5 and 4.5 g.L-1).In the Pb(NO3)2 and Ca(NO3)2combined treatment,0.1-1.0 g.L-1Ca(NO3)2 could relieved the poison of 50mg.L-1Pb(NO3)2.
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备注/Memo
第一作者简介:杨丽娟(1965-),女,硕士,副教授,主要从事植物细胞生物学研究。E-mail:thpgjw@163.com。