[1]韩英鹏,赵 雪,李修平,等.大豆种质对花叶病毒病和疫霉根腐病抗病性的SSR标记辅助鉴定[J].大豆科学,2014,33(01):27-30.[doi:10.11861/j.issn.1000-9841.2014.01.0027]
　HAN Yingpeng,ZHAO Xue,LI Xiuping,et al.SSR Identification of Soybean Cultivar with Resistance to Soybean Mosaic Virus and Phytophthpra Root Rot[J].Soybean Science,2014,33(01):27-30.[doi:10.11861/j.issn.1000-9841.2014.01.0027]
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大豆种质对花叶病毒病和疫霉根腐病抗病性的SSR标记辅助鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年01期 页码: 27-30 栏目: 出版日期: 2014-02-25
Title:
SSR Identification of Soybean Cultivar with Resistance to Soybean Mosaic Virus and Phytophthpra Root Rot
文章编号:
10009841（2014）01002704
作者:
韩英鹏; 赵 雪; 李修平; 滕卫丽; 李文滨
东北农业大学大豆生物学教育部重点实验室，东北农业大学农业部东北大豆生物学与遗传育种重点实验室，黑龙江 哈尔滨 150030
Author(s):
HAN Yingpeng; ZHAO Xue; LI Xiuping; TENG Weili; LI Wenbin
Key Laboratory of Soybean Biology in Chinese Ministry of Education,Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry,Northeast Agricultural University,Harbin 150030,China
关键词:
大豆花叶病毒病; 大豆疫霉根腐病; 抗病性; SSR标记鉴定
Keywords:
Soybean mosaic virus; Phytophthora root rot; Resistance; SSR identification
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.01.0027
文献标志码:
A
摘要:
通过与花叶病毒病（soybean mosaic virus,SMV）抗性相关的SSR标记Satt114对30份大豆种质进行分子辅助鉴定，共发现10个抗SMV的种质，经摩擦接种法表型验证其中9个种质表现为抗病，即分子辅助鉴定的准确性为90%。另外，利用与疫霉根腐病(phytophthora root rot，PRR)相关的SSR标记Satt325、Satt343、Satt428、Satt005、Satt600、Satt611、Satt689、Satt579、Satt252、Satt274对相同的30份大豆种质进行分子辅助鉴定，共在2，3，5，6，12，2份大豆种质上分别检测到0，1，2，3，4，5个抗PRR的QTL，并利用菌土法盆栽验证种质的抗病性，结果表明拥有0～1个抗PRR的QTL大豆种质的病害损失率平均为94%，拥有2～3个抗PRR的QTL大豆种质的病害损失率平均为77.82%，拥有4～5 个抗PRR的QTL大豆种质的病害损失率平均为36.70%。通过本研究筛选获得了1份既高抗SMV又高抗PRR的种质合0531。
Abstract:
One SSR(Satt114),associated with soybean mosaic virus(SMV),ten SSR markers(Satt325,Satt343,Satt428,Satt005,Satt600,Satt611,Satt689,Satt579,Satt252,Satt274),associated with phyphthora root rot(PRR),were used in this study.Ten cultivars resistance to SMV were identified through SSR marker Satt114,9 of which were validated by phenotypic evaluation，and the accurate rate of its marker assistant selection was 90%.In addition,2,3,5,6,12,and 2 cultivars with resistance to PRR was identified,which had 0,1,2,3,4,5 resistance locus,respectively.The disease loss rate of cultivar with 01,23,and 45 QTLs,was 94%,77.82% and 36.70%,respectively.H0531 was a cultivar with resistance to both SMV and PRR.

参考文献/References:

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备注/Memo

基金项目： 国家重点基础研究发展计划“973计划”前期项目（2012CB126311）；国家自然科学基金（31201227）；国家“十二五”科技支撑计划（2011BAD35B061）；现代农业产业技术体系项目（CARS04PS04）； 中国博士后项目（20110491024）；黑龙江省博士后项目(LBH11220，LBHTZ1210)；黑龙江省教育厅骨干教师项目（1252G014）；黑龙江省教育厅新世纪项目（1253NCET005）；教育部博士点项目（20122325120012）；东北农业大学博士后启动金项目（2012RCB11）。

第一作者简介： 韩英鹏(1978)，男，副教授，主要从事分子辅助育种研究工作。Email:hyp234286@aliyun.com。?
通讯作者： 李文滨（1958），男，教授， 主要从事大豆遗传育种和作物生物技术研究工作。Email:wenbinli@yahoo.com。

更新日期/Last Update: 2014-08-04