[1]厉 志,王曙明,刘 佳,等.广适性转bar基因大豆除草剂草丁膦筛选浓度的研究[J].大豆科学,2013,32(06):810-813.[doi:10.11861/j.issn.1000-9841.2013.06.0810]
　LI zhi,WANG Shu-ming,LIU Jia,et al.Study on Screening Concentration of Wide Adaptability Herbicide Resistant? bar Transgenic Soybean[J].Soybean Science,2013,32(06):810-813.[doi:10.11861/j.issn.1000-9841.2013.06.0810]
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广适性转bar基因大豆除草剂草丁膦筛选浓度的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年06期 页码: 810-813 栏目: 出版日期: 2013-12-25
Title:
Study on Screening Concentration of Wide Adaptability Herbicide Resistant? bar Transgenic Soybean
作者:
?厉 志1; 2; 王曙明1; 2; 刘 佳1; 孟凡梅1; 衣志刚1; 董志敏1; 2
?（1.吉林省农业科学院 大豆研究所，吉林 长春 130033； 2.国家大豆工程研究中心，吉林 长春 130033）
Author(s):
?LI zhi1; 2; WANG Shu-ming1; 2; LIU Jia1; MENG Fan-mei1; YI Zhi-gang1; DONG Zhi-min1; 2
?(1.Soybean Research Institute,Jilin Academy of Agricultural Sciences,Changchun 130033,China；2.National Engineering Research Center for Soybean,Changchun 130033,China）
关键词:
bar基因; 转基因大豆; 除草剂; 草丁膦; 筛选
Keywords:
bar gene; Transgenic soybean; Herbicide; Glufosinate; Screening
DOI:
10.11861/j.issn.1000-9841.2013.06.0810
文献标志码:
A
摘要:
?草丁膦作为转基因筛选剂具有高效、快速、简便等优点。为了提高筛选转基因大豆阳性植株的效率，以6个常用的转基因受体大豆品种为材料，在温室和田间，采用叶片涂抹和整株喷施两种方法，通过受体品种叶片颜色变化和致死浓度确定了转基因后代材料适宜筛选浓度，其中适宜涂抹浓度为100~150 mg·L-1，适宜喷施浓度为700~750 mg·L-1。以转bar基因T1代大豆植株进行验证，筛选浓度筛选准确率分别达到61.3%和73.9%。本研究为抗草丁膦转基因大豆后代材料的筛选提供了重要依据。
Abstract:
?Glufosinate proves to be effective and convenient in screening transgenic soybean plants.To improve the screening efficiency of transgenic positive plants,the glufosinate screening concentrations were determined by two methods(daubing and spraying)in the greenhouse and field,with six common soybean transgenic receptor varieties as materials.The suitable glufosinate screening concentrations for daubing and spraying were 100-150 and 700-750 mg·L-1,respectively,when determined by leaves color changes and mortality rate of soybean plants.Then the availability was tested by T1 plants of soybean transgenic lines with bar gene,the accuracy rate was 61.3% and 73.9%,respectively.The results would facilitate the screening of glufosinate resistant transgenic soybean progenies.

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备注/Memo

?转基因生物新品种培育重大专项（2013ZX08004-001-05；2014ZX08004-002B-003）。

更新日期/Last Update: 2014-04-04