[1]王 舒,韩岚岚,赵 寅,等.八字地老虎GOBP1基因的克隆、序列分析与原核表达[J].大豆科学,2013,32(04):467-472.[doi:10.11861/j.issn.1000-9841.2013.04.0467]
　WANG Shu,HAN Lan-lan,ZHAO Yin,et al.Molecular Cloning,Sequence Analysis and Prokaryotic Expression of General Odorant Binding Protein 1 Gene from Agrotis c-nigrum[J].Soybean Science,2013,32(04):467-472.[doi:10.11861/j.issn.1000-9841.2013.04.0467]
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八字地老虎GOBP1基因的克隆、序列分析与原核表达

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年04期 页码: 467-472 栏目: 出版日期: 2013-08-25
Title:
Molecular Cloning,Sequence Analysis and Prokaryotic Expression of General Odorant Binding Protein 1 Gene from Agrotis c-nigrum
作者:
?王 舒1; 韩岚岚1; 赵 寅2; 赵奎军1
?1.东北农业大学 农学院，黑龙江 哈尔滨 150030；2.黑龙江省富裕县农业技术推广中心，黑龙江 富裕县 161200
Author(s):
?WANG Shu1;  HAN Lan-lan1;  ZHAO Yin2;  ZHAO Kui-jun1
?1.College of Agriculture,Northeast Agricultural University,Harbin 150030,China;2.Fuyu County Agricultural Technology Extension Center,Fuyu County 150030,China
关键词:
大豆; 八字地老虎; 克隆; 原核表达; 序列分析
Keywords:
Soybean; Agrotis c-nigrum; General odorant binding protein 1; Cloning; Prokaryotic expression; Sequence analysis
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2013.04.0467
文献标志码:
A
摘要:
?GOBP1蛋白作为昆虫化学感受机制的重要蛋白，对于研究气味调控防治害虫有重要意义。采用RTPCR技术以八字地老虎的触角总RNA为模板进行克隆得到1条1 260 bp的八字地老虎GOBP1基因片段（GenBank登录号为JX978464），编码161个氨基酸残基，预测分子量约为18.6 kD，等电点5.14。生物信息学在线预测分析表明：八字地老虎GOBP1核苷酸序列与黄地老虎Agrotis segetum（登录号为DQ838577.1）同源性最高，为97%，与其他昆虫的同源性达80%以上。构建了重组表达质粒pET21-b/Acni GOBP1原核表达后检测到18.6 kD的外源蛋白，Western bolt检测该蛋白是目的蛋白。结果为深入研究GOBP1基因以及通过气味调控防治害虫提供了基础资料。
Abstract:
?General odorant binding protein 1(GOBP1) is the important protein of insect chemosensory mechanism,and it has great significance for researching odor control of pest.In this test,a 1 260 bp gene fragment was cloned by using the technology of RTPCR with template of Agrotis cnigrum tentacles RNA(GenBank Accession Number:JX978464),which encoded 161 amino acid residues with 18.6 kD predicted molecular weight and pI 5.14.Online bioinformatics analysis showed nucleotide sequence homology of Agrotis c-nigrum GOBP1 with Agrotis segetum(Accession Number:DQ838577.1)reached 97%,and the similarity with other insects was higher than 80%.Western bolt detection showed it was the target protein.These results provide the basic data for the pest bio-control with odor control and the research of GOBP1.

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备注/Memo

?基金项目：现代农业产业技术体系建设专项（CARS04）；公益性行业（农业）专项资助（201103002）。

更新日期/Last Update: 2014-02-17