[1]赵 艳,赵 丹,刘 博,等.大豆P39-1基因及其启动子表达方式的初步研究[J].大豆科学,2013,32(03):425-428.[doi:10.11861/j.issn.1000-9841.2013.03.0425]
　ZHAO Yan,ZHAO Dan,LIU Bo,et al.Preliminary Studies on the Expression Pattern of Soybean P39-1 Gene and Promoter[J].Soybean Science,2013,32(03):425-428.[doi:10.11861/j.issn.1000-9841.2013.03.0425]
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大豆P39-1基因及其启动子表达方式的初步研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年03期 页码: 425-428 栏目: 出版日期: 2013-06-25
Title:
Preliminary Studies on the Expression Pattern of Soybean P39-1 Gene and Promoter
作者:
赵 艳1; 赵 丹2; 刘 博1; 国春晖1
(1.齐齐哈尔大学 生命科学与农林学院，黑龙江 齐齐哈尔 161006；2.吉林市第七中学，吉林 吉林 132011)
Author(s):
ZHAO Yan1; ZHAO Dan2; LIU Bo1; GUO Chun-hui1
(1.College of Life Science and Agroforestry,Qiqihar University,Qiqihar 161006,China;2.Jilin City No.7 Middle School,Jilin 132011,China)
关键词:
大豆; P39-1基因; 启动子; 克隆
Keywords:
Soybean; P39-1 gene; Promoter; Cloning
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2013.03.0425
文献标志码:
A
摘要:
利用实时定量PCR方法，检测大豆P39-1基因在大豆各组织中的表达方式，结果显示该基因在大豆根、茎、叶中的表达活性低，而在花和种子中的相对表达活性极高。利用PCR方法，克隆大豆P39-1基因5′端上游2 000 bp序列，命名为P39-1-p。在线启动子预测分析表明P39-1-p序列中含有多种典型的种子特异表达元件和花特异表达的元件，如SEF1 motif、SEF3motif、SEF4 motif、E-box、G-box、AACACA、AACA、ACGT、CCAA；52-box、ntp303-box、GTGA、TACPyATbox，推测大豆P39-1-p启动子具有调控下游基因大量表达在花和种子中的特性。
Abstract:
The expression pattern of soybean P39-1 gene in soybean tissues was detected by real-time quantitative PCR(RTQ-PCR),and the result showed that it had lower activity in roots,stems and leaves,while higher activity in flowers and seeds.The 5’flanking upstream sequence of soybean P39-1 gene,named P39-1-p,was isolated from soybean genomic DNA by PCR method,and the length was 2 000 bp.Sequence analysis revealed that this fragment contained a series of motifs related to seed specific promoters and some pollenexpressed elements,such as SEF1 motif,SEF3motif,SEF4 motif,E-box,G-box,AACACA,AACA,ACGT,CCAA；52-box,ntp303-box,GTGA and TACPyATbox.It is inferred that P39-1-p promoter possesses the function driven downstream gene expression abundantly in soybean flowers and seeds.

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更新日期/Last Update: 2014-03-04