[1]吴 冰,李喜焕,刘 翠,等.大豆MYB转录因子GmPHR1转化及功能研究[J].大豆科学,2013,32(03):302-305.[doi:10.11861/j.issn.1000-9841.2013.03.0302]
　WU Bing,LI Xi-huan,LIU Cui,et al.Genetic Transformation and Function Analysis of Transcription Factor GmPHR1 in Soybean[J].Soybean Science,2013,32(03):302-305.[doi:10.11861/j.issn.1000-9841.2013.03.0302]
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大豆MYB转录因子GmPHR1转化及功能研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年03期 页码: 302-305 栏目: 出版日期: 2013-06-25
Title:
Genetic Transformation and Function Analysis of Transcription Factor GmPHR1 in Soybean
作者:
?吴 冰; 李喜焕; 刘 翠; 王运杰; 李文龙; 常文锁; 张彩英
?河北农业大学/教育部华北作物种质资源研究与利用重点实验室，河北 保定 071001
Author(s):
?WU Bing; LI Xi-huan; LIU Cui; WANG Yun-jie; LI Wen-long; CHANG Wen-suo; ZHANG Cai-ying
?North China Key Laboratory of Crop Germplasm Resources,Education Ministry of China/Agricultural University of Hebei,Baoding 071001,China
关键词:
大豆; MYB转录因子; 载体构建; 农杆菌转化; 高效表达
Keywords:
Soybean; MYB transcription factor; Vector construction; Agrobacterium mediated transformation; Highly expressed
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2013.03.0302
文献标志码:
A
摘要:
?在克隆获得MYB转录因子GmPHR1基础上，构建基因超表达载体，采用农杆菌介导子叶节转化技术将其转入冀豆12，获得GmPHR1高效表达转基因新材料，并分析基因在耐低磷和低磷敏感品种中的表达差异。结果表明：成功构建了目的基因超表达载体pBI121-GmPHR1，获得了经PCR验证为阳性的T4转基因新材料；荧光实时定量PCR检测发现，其中4个株系的GmPHR1表达量达到野生型对照的2倍以上，说明GmPHR1能够在转基因新材料中高效表达；进一步分析低磷胁迫下不同耐低磷特性品种的基因表达量，发现GmPHR1在耐低磷品种中呈现快速诱导、持续表达与高表达量的模式，而在低磷敏感品种中则表现缓慢诱导、迅速下降和低表达量的模式。
Abstract:
?To develop new transgenic soybean materials with MYB transcription factor GmPHR1, and to study the expression patterns of GmPHR1 in different low P tolerant soybean varieties,a over expression vector pBI121 GmPHR1 was constructed and introduced into Jidou12 by Agrobacterium mediated cotyledonarynode transformation method.PCR and realtime quantitative PCR analysis results of the transformed T4 plants showed that the GmPHR1 was successfully incorporated into the soybean genome and expressed at a higher level than the wild-type.Expression patterns assayed by real-time quantitative PCR revealed that the expression level of GmPHR1 in low P tolerant variety NF37 was induced quickly for a longer period at a higher level than that in sensitive variety Niumaohuang under phosphate starvation condition.

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更新日期/Last Update: 2014-03-04