SHI Feng-mei,MENG Qing-lin,MA Li-gong,et al.Biological Characters of Sclerotinia Sclerotiorum in Soybean[J].Soybean Science,2013,32(02):224-228.[doi:10.3969/j.issn.1000-9841.2013.02.019]
大豆菌核病菌生物学特性的研究
- Title:
- Biological Characters of Sclerotinia Sclerotiorum in Soybean
- Keywords:
- Soybean; Sclerotinia sclerotiorum; Biological characteristic
- 文献标志码:
- A
- 摘要:
- 为有效控制大豆菌核病的发生、蔓延，从采自黑龙江省不同地区大豆菌核病菌中选取5个代表性菌株进行生物学特性的研究。结果表明：大豆菌核病菌菌丝生长的最适温度为20～25℃，而菌核在15～20℃时产生量最多；菌丝适于在中性和偏酸性条件下生长。病菌在PDA和大豆叶片汁培养基上生长较好，菌核的产生则在向日葵叶片汁培养基上最多；有无光照对菌丝生长影响不大，但光照有利于菌核的产生。子囊孢子萌发最适温度为25℃；萌发最适pH为7~8；大豆汁、向日葵汁和油菜汁可促进子囊孢子的萌发；有无光照对子囊孢子萌发无显著影响。不同地区大豆菌核病菌由于生态条件的不同，菌核萌发形成子囊盘能力和病原菌的致病力也存在差异。
- Abstract:
- Five typical isolates of Sclerotinia sclerotiorum in soybean collected from different locations of Heilongjiang province were tested about biological characteristics to effectively control the occurrence and extension of Sclerotinia sclerotiorum.The results showed that optimal temperature of mycelial growth and sclerotia production was at 2025℃and 1520℃，respectively.The suitable condition of mycelial growth was neutral and acidic.The optimal medium of mycelial growth and sclerotia production was PDA，soybean leaves juice and sunflower leaves juice，respectively.Light had no significant effect on mycelial growth，but was beneficial to sclerotia production.The optimal temperature of ascospore germination was 25℃，the optimum pH was 78.Soybean，sunflower and rape juice could promote ascospore germination.Light had no significant effect on the germination of ascospores.Due to different ecological conditions，S.sclerotiorum isolated from various locations were differed in apothecia production and pathogenicity.
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备注/Memo
?国家公益性行业（农业）科研专项（201103016）。