[1]李维娜,李永光,盖江南,等.甲基紫精胁迫下转GLP7基因大豆抗氧化性的初步分析[J].大豆科学,2013,32(02):149-153.[doi:10.3969/j.issn.1000-9841.2013.02.003]
　LI Wei-na,LI Yong-guang,GAI Jiang-nan,et al.Analysis of Antioxidant System Activities in Transgenic Soybean Transformed with GLP7 under Methyl Viologen Stress[J].Soybean Science,2013,32(02):149-153.[doi:10.3969/j.issn.1000-9841.2013.02.003]
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甲基紫精胁迫下转GLP7基因大豆抗氧化性的初步分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年02期 页码: 149-153 栏目: 出版日期: 2013-04-25
Title:
Analysis of Antioxidant System Activities in Transgenic Soybean Transformed with GLP7 under Methyl Viologen Stress
作者:
李维娜; 李永光; 盖江南; 王朋朋; 王 涛; 李文滨
（大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室/东北农业大学 大豆研究所,黑龙江 哈尔滨 150030）
Author(s):
LI Wei-na; LI Yong-guang; GAI Jiang-nan; WANG Peng-peng; WANG Tao; LI Wen-bin
关键词:
GLP7基因; 大豆; MV; 氧化胁迫; 叶绿素; 抗氧化酶
Keywords:
GLP7 gene; Soybean; MV; Oxidative stress; Chlorophyll; Antioxidant enzymes
DOI:
10.3969/j.issn.1000-9841.2013.02.003
文献标志码:
A
摘要:
为探讨GLP7基因参与植物抗氧化胁迫的作用机制及其生物学功能，以转GLP7基因大豆T2代株系及其受体品种垦丰16为材料，在氧化剂甲基紫精（MV）胁迫条件下，测定植株叶绿素、丙二醛(MDA)含量及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性的变化情况。结果表明：相对于非转基因大豆，转基因大豆在MV氧化胁迫下MDA含量降低，总叶绿素含量增加，SOD、CAT活性显著提高。初步证明GLP7的过量表达提高了大豆的抗氧化能力，降低了氧化胁迫对大豆幼苗的损伤。
Abstract:
To discuss the function mechanism of GLP7 gene in antioxidation stress and its biological function,GLP7 transgenic lines of soybean and its acceptor cultivar Kenfeng 16(WT)were used to measure activities of superoxide dismutase(SOD),catalase(CAT),chlorophyll and(MDA)contents under oxidant methyl viologen(MV)stress.The results showed that,compared with nontransgenic soybeans,transgenic soybeans reduced MDA content,increased total chlorophyll content,SOD and CAT activity in MV stress treatment.These results indicated that the over expression of GLP7could effectively enhance the activities of antioxidant ability and reduce the damage on the soybean seedlings under oxidative stress.

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备注/Memo

?抗逆转基因大豆新品种培育重大专项（2011ZX08004-002）。

更新日期/Last Update: 2014-01-23