[1]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38-45.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(01):38-45.[doi:10.3969/j.issn.1000-9841.2013.01.009]
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大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年01期 页码: 38-45 栏目: 出版日期: 2013-02-25
Title:
Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment
文章编号:
1000-9841(2013)01-0038-05
作者:
盖江南;张彬彬;吴瑶;李文滨
东北农业大学大豆生物学教育部重点实验室东北农业大学农业部东北大豆生物学与遗传育种重点实验室;
Author(s):
GAI Jiang-nan; ZHANG Bin-bin; WU Yao; LI Wen-bin
Key Laboratory of Soybean Biology in Chinese Ministry of Education,Key Laboratory of Biology and Breeding/ Genetics of Chinese Agriculture Ministry,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
关键词:
大豆; 不定胚悬浮培养; 基因枪; 基因型筛选
Keywords:
Soybean; Adventitious embryos suspension system; Particle bombardment; Genotype screening
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.009
摘要:
对32个不同基因型大豆的幼胚进行不定胚诱导,30 d时统计分析初生胚诱导率、次生胚诱导率和诱导效率,经综合比较得出适合体细胞诱导不定胚的4种基因型为L155、垦丰16、绥农25和吉育91。然后以不定胚诱导率较高的L155为材料建立大豆悬浮培养不定胚繁殖体系,并用基因枪轰击的方法将GUS表达盒片段转入不定胚细胞团。X-gluc染色后显微观察到基因蓝色产物,说明GUS基因通过微弹轰击法导入大豆不定胚细胞并表达。
Abstract:
In this work,we used the immature cotyledons of 32 soybean genotypes to induce adventitious embryos and compared the percentage of primary embryo induction,percentage of secondary embryo induction and induction efficiency after 30 days inducing culture.The results showed that L155,Kenfeng 16,Suinong 25 and Jiyu 91 had higher frequency of adventitious embryos induction.Furthermore,L155 was selected to further establish the soybean suspension culture system.The secondary embryogenesis systems were used for GUS gene transformation by particle gun bombardment method.The GUS staining results showed that the GUS gene was imported into soybean adventitious embryos cells and successfully expressed.

参考文献/References:

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备注/Memo

基金项目：抗逆转基因大豆培育专项（2011ZX08004-002）。

第一作者简介：盖江南（1987-），女，在读硕士，研究方向为大豆遗传育种。E-mail：little35friend@163.com。
通讯作者：李文滨（1958-），男，教授，博士生导师，主要从事大豆遗传育种研究。E-mail:wenbinli@neau.edu.cn。

更新日期/Last Update: 2013-02-20