[1]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8-17.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(01):8-17.[doi:10.3969/j.issn.1000-9841.2013.01.003]
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大豆脂质转运蛋白基因GmLTP3的特征分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年01期 页码: 8-17 栏目: 出版日期: 2013-02-25
Title:
Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max
文章编号:
1000-9841-2013-01-0008-04
作者:
李彩云1; 2; 余永亮2; 杨红旗2; 董薇2; 田保明1; 梁慧珍2
1.郑州大学 生物工程系，河南 郑州 450001；
2.河南省农业科学院 芝麻研究中心，河南 郑州 450002
Author(s):
LI Cai-yun1; 2;  YU Yong-liang2;  YANG Hong-qi2;  DONG Wei2;  TIAN Bao-ming1;  LIANG Hui-zhen2
1.Department of Bioengineering,Zhengzhou University,Zhengzhou 450001,Henan;
2.Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China
关键词:
大豆; 脂质转运蛋白; 非生物胁迫; 表达分析
Keywords:
Glycine max; Lipid-transfer proteins; Abiotic stress; Expression analysis
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.003
摘要:
将芝麻的SiLTP3序列与大豆的核苷酸序列进行blast比对,发现大豆含有与芝麻该基因同源性高达94%的序列,克隆该基因并命名为GmLTP3。通过GmLTP3的核苷酸序列推测出其氨基酸序列,利用软件进行多重序列比对和系统发育树构建,发现该氨基酸序列与已确定功能的大豆脂质转运蛋白同源性高达99%,与蒺藜苜蓿和巴旦杏的进化距离最近。采用实时荧光定量PCR法对GmLTP3基因进行定量检测,组织表达分析表明,GmLTP3属于组成性表达基因,在大豆的根、茎、叶、花、萌发的种子中均有表达,并且在花蕾、茎和萌发的种子中表达量较高,在根、叶中表达量较低。非生物胁迫诱导表达分析表明,GmLTP3基因能够对IAA、ABA、PEG、NaCl和冷害作出应答。胁迫处理2 h后,GmLTP3表达量均有下调趋势;胁迫处理12 h后,IAA、ABA、PEG、NaCl诱导GmLTP3的表达量均有不同程度的上调,冷害处理则继续下调表达量。
Abstract:
In this study,a gene which have 94% identity with the SiLTP3 was cloned and named as GmLTP3. GmLTP3 encodes a lipid-transfer protein(LTP)that have 99% identity with the known Glycine max lipid transfer protein.The transcriptional level of GmLTP3 was quantitatively determined using realtime-PCR,it was expressed in almost all the tissues,such as root,stem,leave,flower and seed.It was highly expressed in alabastrum,7-days old stem and 3-days germinating seed,while it was lowly expressed in root and leave.When treated by abiotic stress,such as IAA,ABA,PEG and NaCl,the expression level was down-regulated after treated 2 hours,but was up-regulated after treated 12 hours,while it was downregulated consistently after treated by cold stress.

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备注/Memo

基金项目：河南省科技创新人才计划（114200510002）；转基因生物新品种培育科技重大专项（2011ZX08004-005）；引进国际先进农业科学技术计划“948计划”项目（2011-G1-13）。

第一作者简介：李彩云(1987-)，女，在读硕士，研究方向为作物育种。E-mail：503600139@qq.com。
通讯作者：梁慧珍(1968-)，女，研究员，博士，主要从事大豆遗传育种工作。E-mail：lhzh66666@163.com。

更新日期/Last Update: 2013-02-20