SONG Jie,XU Yan-li,YAO Qin,et al.Suppression of Urea on Soybean Cyst Nematode[J].Soybean Science,2012,31(05):784-788.[doi:10.3969/j.issn.1000-9841.2012.05.020]
尿素对大豆胞囊线虫的抑制作用
- Title:
- Suppression of Urea on Soybean Cyst Nematode
- 文章编号:
- 1000-9841（2012）05-0784-05
- Keywords:
- Soybean Cyst Nematode; Urea; Egg hatch; J2
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 采用实验室生测和温室盆栽的方法，研究了尿素对大豆胞囊线虫卵孵化、二龄幼虫活性的抑制作用以及对大豆胞囊线虫的控制效果。结果显示，尿素的浓度在0.03～1.00 mol·L-1范围内，对大豆胞囊线虫的卵孵化和二龄幼虫（J2）均具有抑制作用，且随着浓度的升高抑制作用增强，当尿素浓度到达1.0 mol·L-1时，卵孵化的抑制率最高达到96%，J2的致死率可达到100%。温室盆栽结果显示，施用尿素后，土中和大豆根部的大豆胞囊线虫种群密度显著降低，且大豆株高、地上鲜重和地下鲜重等均有所增加，其中鲜重总增重率可达42.8%。研究结果证明尿素对大豆胞囊线虫具有明显的致死作用，且在一定程度上能控制该病害的发生。
- Abstract:
- A study was conducted in lab and greenhouse to determine the effect of the urea on eggs,second-stage juveniles(J2)of Heterodera glycines and control efficiency of Soybean Cyst Nematode(SCN).The results showed that the urea inhibited the nematode egg hatch and the activity of J2 at the concentration of urea from 0.03 to 1.0 mol·L-1,and with the increase of concentration,the suppressive effect rose accordingly.The suppressive rate of egg hatching and mortality of J2 reached 96% and 100% when urea concentration was at 1.0 mol·L-1,respectively.In greenhouse,application of urea significantly reduced the population of SCN and the population of SCN in soil and soybean root.The application of urea also enhanced height,fresh and dry weight of shoots and roots of soybean,and the increase rate of fresh weight was 42.8%.The urea has lethal effect on SCN,and the population of SCN is suppressed by urea in a certain extent.
参考文献/References:
[1]刘维志.植物病原线虫学[M].中国农业出版社，2000：28l-294.（Liu W Z.Plant pathogenic nematology[M].Beijing:China Agricultural Press,2000:28l-294.) [2]许艳丽.土壤环境对大豆胞囊线虫卵孵化影响及线虫分子诊断研究[D].哈尔滨：东北农业大学，2004.(Xu Y L.The effects of soil environment on Soybean Cyst Nematode egg hatch and nematode molecular diagnosis[D].Harbin:Northeast Agricultural University,2004.) [3]司兆胜.土壤环境对大豆胞囊线虫繁殖影响及大豆抗性机制研究[D].哈尔滨：东北农业大学，2004.(Si Z S.The effect of environment situation on soybean cyst nematode hatch and the mechanism of soybean resisting Soybean Cyst Nematode[D].Harbin:Northeast Agricultural University,2004.) [4]邢丽娟，刘维志，段玉玺，等.某些阳离子在大豆胞囊线虫生防系统中的作用[J].中国生物防治，2002:18(1):1-5.(Xing L J,Liu W Z,Duan Y X,et al.Influence of some ions on biocontrol system of Soybean Cyst Nematode[J].Chinese Journal of Biological Control,2002:18(1):1-5.) [5]Young L D.Soybeans resistant to Heterodera glycines populations attacking Hartwig soybean[J].Journal of Nematology,1999:31∶583. [6]郑雅楠，段玉玺，陈立杰.大豆胞囊线虫二龄幼虫对含氮离子及化合物适应性的初步研究[C].中国植物病理学会学术年会论文集，2007:289-292.(Zheng Y N,Duan Y X,Chen L J.The effect of nitrogen ion and compound on Soybean Cyst Nematode J2[C].Proceedings of the Annual Meeting of Chinese Society for Plant Pathoiogy,2007:289-292.) [7]Barker K R, Koenning S R. Developing sustainable systems for nematode management[J].Annual Review of Phytopathology,1998:36:165-205. [8]孙漫红，刘杏忠，晋治波.淡紫拟青霉对大豆胞囊线虫卵及2龄幼虫的影响[J].植物保护学报，2002:29(1):58.（Sun M H,Liu X Z,Jin Z B.Effect of Paecilomyces lilacinue?on egg hatching and juvenile morality of Heterodera glycines[J].Acta Phytophylacica Sinica,2002:29(1):58.) [9]Behm J E,Tylka G L,Niblack T L.Effects of fertilization of corn on hatching of Heterodera glycines in soil[J].Journal of Nematology,1995:27:164-171. [10]靳学慧，辛惠普，郑雯，等.长期轮作和连作对土壤中大豆胞囊线虫数量的影响[J].中国油料作物学报，2006，28（2）：189-193.(Jin X H,Xin H P,Zheng W,et al.The influence of soil on the long-term rotation and continuous cultivation on Soybean Cyst Nematode[J].Chinese Journal of Oil Crop Sciences,2006，28（2）：189-193.) [11]甘银波，涂学文，田任久.大豆的最佳氮肥施用时期研究[J].大豆科学，1998，17(4):287.(Gan J B,Xu X W,Tian J R.Study on optimum timing of nitrogen application on soybean[J].Soybean Science,1998,17(4):287.) [12]Nakasathien S,Israel D W,Wilson R F,et al.Regulation of seed protein concentration in soybean by supra-optimal nitrogen supply[J].Crop Science,2000,40:1277-1284. [13]Kronzucker H J,Britto D T,Davenport R J，et al.Ammonium toxicity and the real cost of transport[J].Trends in Plant Science,2001,6:335-337.
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备注/Memo
基金项目：国家自然科学基金项目（30971900）；黑龙江省自然科学基金项目（C200630）。