ZHAO Yan,QI Hong-ying,FAN Zhen-yu,et al.Preliminary Analysis of the Expression Pattern of Soybean acyl-ACP-Thioesterase Gene and Promoter[J].Soybean Science,2012,31(05):714-717.[doi:10.3969/j.issn.1000-9841.2012.05.005]
大豆酰基载体蛋白硫酯酶基因及其启动子表达方式的初步分析
- Title:
- Preliminary Analysis of the Expression Pattern of Soybean acyl-ACP-Thioesterase Gene and Promoter
- 文章编号:
- 1000-9841(2012)05-0714-04
- 关键词:
- 大豆; 酰基载体蛋白硫酯酶基因; 启动子; 克隆
- Keywords:
- Soybean; acyl-ACP thioesterase; Promoter; Cloning
- 分类号:
- Q756
- 文献标志码:
- A
- 摘要:
- 利用实时定量PCR方法,检测大豆酰基载体蛋白硫酯酶(acyl-ACP thioesterase)基因在大豆各组织中的表达方式,结果显示该基因在大豆根、茎、叶、花中的表达活性低,而种子中的表达活性较高。利用PCR方法,克隆大豆acyl-ACP thioesterase基因5′端上游2 057 bp序列,命名为AP。在线启动子预测软件分析结果表明AP序列中含有多种典型的种子特异表达元件,如RY repeat、SEF1 motif、SEF3 motif、SEF4 motif、E-box、ACGT等顺式作用元件,推测大豆acyl-ACP thioesterase基因启动子具有种子特异表达活性。
- Abstract:
- The expression pattern of soybean acyl-ACP thioesterase gene in soybean tissues was detected by real-time quantitative PCR(RTQ-PCR).The result showed that there were a little activity in roots,stems,leaves and flowers,but there was higher activity in seeds.The 5′-flanking upstream sequence of soybean acyl-ACP thioesterase gene,named AP,was isolated from soybean genomic DNA by PCR method,and the length was 2 057 bp.Sequence analysis by PLACE revealed that this fragment contained a series of motifs related to seed-specific promoters,such as RY repeat,SEF1 motif,SEF3 motif,SEF4 motif,E-box and ACGT.It can be inferred that AP promoter possess the function driven downstream gene expression exclusively in soybean seeds.
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备注/Memo
基金项目:齐齐哈尔大学青年教师科研启动支持计划项目(2010K-M09);黑龙江省教育厅科学技术研究项目(12521611);作物生物学国家重点实验室开放课题(2012KF13);国家自然科学基金(40971053)。