LIU Yang,ZHANG Hui,XIN Da-wei,et al.Domain Analysis and Function Prediction of TCP Transcription Factors Family in Soybean[J].Soybean Science,2012,31(05):707-713,717.[doi:10.3969/j.issn.1000-9841.2012.05.004]
大豆TCP转录因子家族结构域分析及功能预测
- Title:
- Domain Analysis and Function Prediction of TCP Transcription Factors Family in Soybean
- 文章编号:
- 1000-9841(2012)05-0707-07
- Keywords:
- Soybean; TCP-domain; Structure domain analysis; Function prediction
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 利用已知的拟南芥TCP基因和大豆基因组数据库,通过生物信息学方法,鉴定并获得了大豆TCP家族基因序列,基因定位信息。共鉴定大豆TCP基因54个,分布在大豆除14号染色体外的其他19条染色体上。对54个基因进行进化和聚类分析及功能结构域的分析,发现全部具有高度保守的TCP结构域。根据结构域差异和系统发育分析的结果,将大豆TCP转录因子家族分成2个TCP亚家族。以生物信息学手段和已有的其他物种的TCP基因进行了比较分析,大豆TCP基因占总数的3.5%,与拟南芥同源基因比较,序列长度存在相似性。对启动子元件分析显示,TCP基因含有大量与在子叶、根、茎处大量表达有关的元件及对分生组织有作用的元件。
- Abstract:
- TCP transcription factors gene sequences were identified based on soybean genome database and Arabidopsis TCP genes by the bioinformatics method.Fifty-four soybean TCP genes all contained highly conservative TCP structure domain,were found and distributed in 19 chromosomes except chromosome No.14.They could be classified into 2 sub-families according to their structure and phylogeny relationship.By compare to TCP genes of other species,the evolution structure was also be analyzed.Soybean TCP genes account for 3.5% of the total amount,and compared with Arabidopsis homologous genes,the sequence length exist similarities.Analysis of promoter elements showed that the TCP gene contains elements,they refer to expression in the cotyledons,roots,stems and has the function of meristem tissue.All results provide a basis for the further study of the soybean TCP transcription factor.
参考文献/References:
[1]Cubas P,Lauter N,Doebley J,et al.The TCP domain:a motif found in proteins regulating plant growth and development[J].Plant Journal,1999,18(2):215-222. [2]Doebley J,Stec A,Gustus C.Teoslnte branched1 and the origin of maize:evidence for epistasis and the evolution of dominance[J].Genetics,1995,141(1):333-346. [3]Luo D,Carpenter R,Vincent C,et al.Origin of floral asymmetry in Antirrhinum[J].Nature,1996,383:794-799. [4]Kosugi S,Ohashi Y.PCF1?and?PCF2?specifically bind to cis elements in the rice proliferating cell nuclear antigen gene[J].Plant Cell,1997,9:1607-1619. [5]雷娟,魏刚,朱玉贤.拟南芥TCP家族转录因子At2g31070的转录激活活性鉴定与表达谱分析[J].分子植物育种,2005(1):31-35.(Lei J,Wei G,Zhu Y X.Arabidopsis?TCP family At2g31070 transcription factors of transcription activate active appraisal and express spectrum analysis[J].Molecular Plant Breeding,2005(1):31-35.) [6]Ababakeli S,Wei G,Lei J,et al.Cloning and trans-activation identification of At1g30210,a new member of TCP family high expressed in flower in Arabidopsis[J].Molecular Plant Breeding,2005,1(6):26-30. [7]刘伟娜,韩建民,董金皋,等.棉花TCP家族转录因子基因GhTCP1的克隆与表达分析[J].棉花学报,2010,22(5):409-414.(Liu W N,Han J M,Dong J G,et al.Cloning and expression analysis cotton TCP family transcription factors GhTCP1 gene[J].Cotton Science,2010,22(5):409-414.) [8]Jiang C J,Imamoto N,Matsuki R,et al.Functional characterization of a plant importin alpha homologue.Nuclear localization signal(NLS)-selective binding and mediation of nuclear import of nls proteins in vitro[J].Journal of Biological Chemistry,1998,273:24083-24087.
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备注/Memo
基金项目:抗除草剂转基因大豆新品种培育项目(2011ZX08004-001-06);现代农业产业体系资助项目(CARS-04-02A);黑龙江省普通高等学校新世纪优秀人才培养计划(1252-NCET-004)。