[1]陈 珣,杨 镇,肖 军,等.植物内生菌醇提取物对大豆凝集素的作用[J].大豆科学,2012,31(03):501-503.[doi:10.3969/j.issn.1000-9841.2012.03.036]
　CHEN Xun,YANG Zhen,XIAO Jun,et al.Effects of Endophytes Alcohol Extraction on Soybean Agglutinin Contents[J].Soybean Science,2012,31(03):501-503.[doi:10.3969/j.issn.1000-9841.2012.03.036]
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植物内生菌醇提取物对大豆凝集素的作用

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年03期 页码: 501-503 栏目: 出版日期: 2012-03-25
Title:
Effects of Endophytes Alcohol Extraction on Soybean Agglutinin Contents
文章编号:
1000-9841（2012）03-0501-03
作者:
陈 珣; 杨 镇; 肖 军; 龚 娜; 王 娜; 王 红; 肇 莹; 杨 涛
辽宁省农业科学院 微生物工程中心，辽宁 沈阳 110161
Author(s):
CHEN Xun; YANG Zhen; XIAO Jun; GONG Na; WANG Na; WANG Hong; ZHAO Ying; YANG Tao
Research Center of Microbiology Engineering,Liaoning Academy of Agricultural Sciences,Shenyang 110161,Liaoning,China
关键词:
大豆; 凝集素含量; 植物内生菌; 血凝法
Keywords:
Soybean; Agglutinin content; Endophytes; Hemagglutination method
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.03.036
文献标志码:
A
摘要:
豆科植物凝集素在豆科植物和根瘤菌的相互识别和共生关系的建立上起着重要作用。利用血凝法快速检测R（人参）、S（沙棘）、D（越橘）3种植物内生菌的S0.25D50、S0.25、R25D25和R50D50共4种处理对大豆根部凝集素含量的影响。结果表明：经过S0.25D50、S0.25、R50D50 3种处理的大豆根部凝集素含量均高于对照，而经过S0.25处理的大豆根部凝集素的含量最高，为0.288 mg·g-1。说明沙棘内生菌醇提取物有利于大豆根瘤菌的形成。
Abstract:
Legume agglutinins play important roles in mutual recognition and establishment of the symbiotic relationship between legumes and rhizobium.In this study,soybean agglutinin contents under four treatments(S0.25D50,S0.25,R25D25 and R50D50)were rapid detected using hemagglutination method.R,S and D were three endophytes from ginseng,sea-buckthorn and cowberry,respectively.The results showed that agglutinin contents in soybean roots under S0.25D50,S0.25 and R50D50 treatments were higher than the control.The highest root agglutinin content was 0.288 mg·g-1 under S0.25 treatment which indicated that alcohol extract of endophyte from sea-buckthorn contributed to the formation of soybean Rhizobium.

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备注/Memo

基金项目：辽宁省科技攻关项目（2011215005）。

第一作者简介：陈珣（1979-），女，硕士，助理研究员，主要从事生物技术研究。E-mail：chenxun_82_ren@126.com。
通讯作者：杨涛（1964-），男，博士，研究员，主要从事生物技术研究。E-mail：smkxzx@sina.com。

更新日期/Last Update: 2014-08-17