XING Cheng-hua,ZHENG Zai-sheng,ZHANG Shang-fa,et al.Protective Effect of Root Border Cells on Salt Stress in Black Soybean[J].Soybean Science,2012,31(03):416-419.[doi:10.3969/j.issn.1000-9841.2012.03.016]
黑豆根尖边缘细胞对盐胁迫的防护效应
- Title:
- Protective Effect of Root Border Cells on Salt Stress in Black Soybean
- 文章编号:
- 1000-9841（2012）03-0416-04
- 分类号:
- Q945.78
- 文献标志码:
- A
- 摘要:
- 采用振荡培养(移除根尖边缘细胞)和静置培养(保持边缘细胞附着在根尖)方法，对比研究盐胁迫对黑豆根系生长和根尖边缘细胞发育、根系Na+、K+含量的影响以及根系生理特性的变化。结果显示：100 和200 mmol·L-1 NaCl处理抑制边缘细胞发育，引起根系相对电导率和MDA含量增加。振荡培养去除根尖边缘细胞处理36 h，黑豆根相对伸长率、根尖K+含量明显低于对应NaCl浓度的静置培养处理，同时根尖Na+含量、相对电导率和MDA含量在去除边缘细胞后显著增加。说明包裹于根尖的边缘细胞通过调节Na+和K+的吸收和维持较高的细胞膜完整性，以适应盐害环境。
- Abstract:
- The effects of salt stress on root growth,root border cells(RBCs)development,Na+ and K+ content and physiological characteristic in black soybean\[Glycine max (L.)Merrill\]were investigated,with RBCs adhered to root tips using a static cultivation method,or with RBCs removed from root tips using a shaking cultivation method.Salt stress treated with 100 and 200 mmol·L-1NaCl inhibited RBCs development and increased relative electric conductivity and MDA content in roots.Physical removal of RBCs from root tips for 36 h resulted in a more severe inhibition of root elongation and a higher Na+ accumulation in the root tips,the relative electric conductivity and MDA content in roots were also significantly increased when RBCs were removed.The results revealed that RBCs adhered to the root tips tended to regulate the uptake of Na+ and K+,and maintain cell membrane integrity,thereby increased salt tolerance in black soybean seedlings.
参考文献/References:
[1] 陈莎莎 , 兰海燕 . 植物对盐胁迫响应的信号转导途径 [J]. 植物生理学报 ,2011,47(2):119-128.(Chen S S,Lan H Y.Signal transduction pathways in response to salt stress in plants[J].Plant Physiology Journal,2011,47(2):119-128.) [2]孙洪刚,陈益泰.沿海防护林四个树种根系分布对盐胁迫的响应[J].生态学杂志,2010,29(12):2365-2372.(Sun H G,Chen Y T.Root growth patterns of four coastal shelter forest tree species in response to salt stress[J].Chinese Journal of Ecology,2010,29(12):2365-2372.) [3]Sekmen A H,Turkan I,Tanyolac Z O,et al.Different antioxidant defense responses to salt stress during germination and vegetative stages of endemic halophyte Gypsophila oblanceolataBark[J].Environmental and Experimental Botany,2012,77:63-76. [4]Gunawardena U,Rodriguez M,Straney D,et al.Tissue-specific localization of pea root infection by?Nectria haematococca.Mechanisms and consequences[J].Plant Physiology,2005,137(4):1363-1374. [5]Jaroszuk-scise1 J,Kurek E,Rodzik B,et al.Interactions between rye(Secale cereale)root border cells(RBCs)and pathogenic and nonpathogenic rhizosphere strains of Fusarium culmorum[J].Mycological Research,2009,113(10):1053-1061. [6]Wuyts N,Maung Z T Z,Swennen R,et al.Banana rhizodeposition:characterization of root border cell production and effects on chemotaxis and motility of the parasitic nematodeRadopholus similes[J].Plant and Soil,2006,283(1-2):217-228. [7]Cai M Z,Wang F M,Li R F,et al.Response and tolerance of root border cells to aluminum toxicity in soybean seedlings[J].Journal of Inorganic Biochemistry,2011,105(7):966-971. [8]Yu M,Shen R F,Liu J Y,et al.The role of root border cells in aluminum resistance of pea(Pisum sativum)grown in mist culture[J].Journal of Plant Nutrition and Soil Science,2009,172(4):528-534. [9]邢承华，张淑娜，吴坤，等.边缘细胞对水稻生长和细胞壁组分的影响及其与耐铝性的关系[J].作物学报,2012,38(4):1-7.(Xing C H,Zhang S N,Wu K,et al.Effects of root border cells on root growth and cell wall polysaccharide contents in rice seedlings and their relation to aluminum tolerance[J].Acta Agronomica Sinica,2012,38(4):1-7.) [10]林植芳,李双顺,林桂珠,等.水稻叶片的衰老与超氧物歧化酶活性及脂质过氧化作用的关系[J].植物学报,1984,26(6):605-615.(Lin Z F，Li S S，Lin G Z,et al.Superoxide dismutase activity and lipid peroxidation in relation to senescence of rice leaves[J].Acta Botanica Sinica,1984,26(6):605-615.) [11]贺莉,徐晓腾,刘菲,等.NaCl胁迫下野生大豆生理生态反应的研究[J].大豆科学,2011,30(2):242-245.(He L,Xu X T,Liu F,et al.Effects of NaCl stress on physio-ecological responses of?Glycine soja[J].Soybean Science,2011,30(2):242-245.) [12]Miyasaka S C,Hawes M C.Possible role of root border cells in detection and avoidance of aluminum toxicity[J].Plant Physiology,2001,125(4):1978-1987. [13]Xing C H,Zhu M H,Cai M Z,et al.Developmental characteristics and response to iron toxicity of root border cells in rice seedlings[J].Journal of Zhejiang University-Science B,2008,9(3):261-264. [14]Llugany M,Lombini A,Poschenrieder C,et al.Different mechanisms account for enhanced copper resistance in?Silene armeriaecotypes from mine spoil and serpentine sites[J].Plant Soil,2003,251(1):55-63. [15]Kong X,Luo Z,Dong H,et al.Effects of non-uniform root zone salinity on water use,Na+ recirculation,and Na+ and H+ flux in cotton[J].Journal of Experimental Botany,2012,63(5):2105-2116. [16]Asensi-Fabado M A,Cela J,Müller M,et al.Enhanced oxidative stress in the ethylene-insensitive(ein3-1)mutant of Arabidopsis thalianaex?posed to salt stress[J].Journal of Plant Physiology,2012,169(4):360-368.
相似文献/References:
[1]张宽朝,阮 飞,金 青,等.刀豆、黑豆脲酶的分离与基本性质研究[J].大豆科学,2013,32(03):361.[doi:10.11861/j.issn.1000-9841.2013.03.0361]
ZHANG Kuan-chao,RUAN Fei,JIN Qing,et al.Separation and Properties of Urease in Sword Bean and Black Soybean[J].Soybean Science,2013,32(03):361.[doi:10.11861/j.issn.1000-9841.2013.03.0361]
[2]陈振武,钱朗,王海英,等.花期追施氮肥对不同株型黑豆品种生殖生长期叶片生理生化特性的影响[J].大豆科学,2010,29(04):623.[doi:10.11861/j.issn.1000-9841.2010.04.0623]
CHEN Zhen-wu,QIAN Lang,WANG Hai-ying,et al.Effect of Nitrogen Fertilizer on Leaf Physiological and Biochemical Traits of Black Soybean during Reproductive Stage[J].Soybean Science,2010,29(03):623.[doi:10.11861/j.issn.1000-9841.2010.04.0623]
[3]杨卫民,刘保琪,李团霞,等.黑豆种皮中红色素的提取及稳定性研究[J].大豆科学,2010,29(04):688.[doi:10.11861/j.issn.1000-9841.2010.04.0688]
YANG Wei-min,LIU Bao-qi,LI Tuan-xia,et al.以黑豆种皮为原料提取红色素，对红色素的提取工艺和稳定性进行了研究。结果表明：以酸性乙醇为提取剂，在60℃条件下提取2 h，方法简便、提取率高达17.36%；黑豆红色素对热、亚硫酸钠、蔗糖、苯甲酸钠以及部分金属离子较稳定，可用于功能食品添加剂、保健药物和化妆品等。[J].Soybean Science,2010,29(03):688.[doi:10.11861/j.issn.1000-9841.2010.04.0688]
[4]钱朗,陈振武,刘柏林,等.氮肥对黑豆产量及氮磷钾积累分配的影响[J].大豆科学,2009,28(01):85.[doi:10.11861/j.issn.1000-9841.2009.01.0085]
QIAN Lang,CHEN Zhen-wu,LIU Bai-lin,et al.Effect of Nitrogen Fertilizer on Yield and Nitrogen，Phosphorous，Potsssium Accumulation and Distrubution of Black Soybean[J].Soybean Science,2009,28(03):85.[doi:10.11861/j.issn.1000-9841.2009.01.0085]
[5]周三,关崎春雄,岳旺,等.野生大豆、黑豆和大豆的异黄酮类成分比较[J].大豆科学,2008,27(02):315.[doi:10.11861/j.issn.1000-9841.2008.02.0315]
ZHOU San,SEKIZAKI Haruo,YUE Wang,et al.Comparison of Isoflavones among Wild，Black and Yellow Soybeans[J].Soybean Science,2008,27(03):315.[doi:10.11861/j.issn.1000-9841.2008.02.0315]
[6]郭婕,胡利宗,李俐俐,等.黑豆豆芽中大豆异黄酮微波提取工艺研究[J].大豆科学,2015,34(03):493.[doi:10.11861/j.issn.1000-9841.2015.03.0485]
GUO Jie,HU Li-zong,LI Li-li,et al.Extraction Technology of Soybean Isoflavones from Black Soybean Sprouts Using Microwave-assisted Extraction[J].Soybean Science,2015,34(03):493.[doi:10.11861/j.issn.1000-9841.2015.03.0485]
[7]郝欣先,蒋惠兰,高建伟,等.大豆抗孢囊线虫病品种选育研究[J].大豆科学,1996,15(02):103.[doi:10.11861/j.issn.1000-9841.1996.02.0103]
[J].Soybean Science,1996,15(03):103.[doi:10.11861/j.issn.1000-9841.1996.02.0103]
[8]张月圆,陈佩瑶,刘露,等.醋制对黑豆蛋白含量及多肽ACE抑制活性的影响[J].大豆科学,2017,36(06):932.[doi:10.11861/j.issn.1000-9841.2017.06.0932]
ZHANG Yue-yuan,CHEN Pei-yao,LIU Lu,et al.Effect of Vinegar Soaking on Black Soybean Protein Fractions Content and Their Corresponding Peptides′ ACE Inhibitory Activity[J].Soybean Science,2017,36(03):932.[doi:10.11861/j.issn.1000-9841.2017.06.0932]
[9]康永锋,薛永刚,韩巧英.响应面优化黑豆总黄酮微波辅助提取工艺及其抗氧化性能评价[J].大豆科学,2017,36(06):958.[doi:10.11861/j.issn.1000-9841.2017.06.0958]
KANG Yong-feng,XUE Yong-gang,HAN Qiao-ying.Optimization of Technology Using Response Surface Analysis for Extracting Flavonoids from Black Bean Assisted by Microwave and Its Antioxidant Activity Evaluation[J].Soybean Science,2017,36(03):958.[doi:10.11861/j.issn.1000-9841.2017.06.0958]
[10]杨晶,肖春萍,胡冬华,等.电磁裂解法提取的黑豆和黄豆异黄酮含量研究[J].大豆科学,2018,37(05):781.[doi:10.11861/j.issn.1000-9841.2018.05.0781]
YANG Jing,XIAO Chun-ping,HU Dong-hua,et al.Study on the Contents of Isoflavones of Black Beans and Soybean Extracted by Electromagnetic Cracking Method[J].Soybean Science,2018,37(03):781.[doi:10.11861/j.issn.1000-9841.2018.05.0781]
备注/Memo
基金项目：国家自然科学基金项目(31101599, 30800705)；浙江省自然科学基金项目(Y3110561)。