[1]杨 喆,唐晓飞,张 颖,等.农杆菌介导不同基因型大豆品种子叶节遗传转化条件的研究[J].大豆科学,2012,31(02):188-192.[doi:10.3969/j.issn.1000-9841.2012.02.006]
　YANG Zhe,TANG Xiao-fei,ZHANG Ying,et al.Optimization on Genetic Transformation Conditions of Agrobacterium-mediated Cotyledonary-nodes from Different Genotype Soybeans[J].Soybean Science,2012,31(02):188-192.[doi:10.3969/j.issn.1000-9841.2012.02.006]
点击复制

农杆菌介导不同基因型大豆品种子叶节遗传转化条件的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年02期 页码: 188-192 栏目: 出版日期: 2012-04-25
Title:
Optimization on Genetic Transformation Conditions of Agrobacterium-mediated Cotyledonary-nodes from Different Genotype Soybeans
文章编号:
1000-9841（2012）02-0188-05
作者:
杨 喆1; 2; 唐晓飞1; 张 颖1; 董兴月1; 王凤义2; 刘丽君1
1.黑龙江省农业科学院 大豆研究所，黑龙江 哈尔滨 150086；
2.东北农业大学 农学院，黑龙江 哈尔滨150030
Author(s):
YANG Zhe1; TANG Xiao-fei1; ZHANG Ying1; DONG Xing-yue1; WANG Feng-yi2; LIU Li-jun1
1.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086;
2.College of Agriculture，Northeast Agricultural University,Harbin 150030,Heilongjiang,China
关键词:
大豆; 不同基因型; 农杆菌介导; 遗传转化条件
Keywords:
Soybean;  Different genotype; Agrobacterium-mediated; Conditions for genetic transformation
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.02.006
文献标志码:
A
摘要:
以黑农46、黑农53和黑农56的子叶节为转化受体，对农杆菌介导大豆子叶节遗传转化中的6-BA浓度、草丁膦浓度、侵染时间、共培养时间以及伸长培养基进行筛选，确定适合不同基因型的最佳转化条件。结果表明：黑农46、黑农53和黑农56的最佳6-BA诱导浓度分别为1.7、1.6 和1.7 mg·L-1；最佳草丁膦筛选浓度分别为2.0、3.0和2.0 mg·L-1；同时确定了侵染时间、共培养时间和伸长培养基类型的最佳组合是侵染时间25 min、共培养时间3 d和Ⅲ型伸长培养基。
Abstract:
Cotyledonary-nodes of Heinong 46,Heinong 53 and Heinong 56 were used as transformation receptors.Culture conditions including 6-BA concentration,glufosinate concentration,infection time,co-culture time and type of elongation medium were screened,in order to find the optimal conditions for Agrobacterium-mediated genetic transformation from different soybean genotypes.Result showed the optimal 6-BA induction concentrations for Heinong 46,Heinong 53 and Heinong 56 were 1.7,1.6 and 1.7 mg·L-1,respectively;the optimal concentrations of glufosinate for Heinong 46,Heinong 53 and Heinong 56 were 2.0,3.0,and 2.0 mg·L-1,respectively;the optimal combination of infection time,co-culture time and type of elongation medium was infection time 25 min,co-culture time 3 d and type Ⅲ of elongation medium.

参考文献/References:

[1]Paz M M,Shou H,Guo Z,et al.Assessment of conditions affecting Agrobacterium -mediated soybean transformation using the cotyledonary node explant[J].Euphytica，2004，136:167-179.

[2]韩雪，韩岚岚，宋雯雯，等.大豆子叶节再生体系的优化与农杆菌转化的研究[J].东北农业大学学报，2010，41(2):1-5.(Han X,Han L L,Song W W,et al.Optimization of regeneration system from cotyledonary nodes of soybean and Agrobacterium-mediated transformation[J].Journal of Northeast Agricultural University,2010，41(2):1-5.)

[3]Liu S J，Wei Z M，Huang J Q.The effect of co-cultivation and selection parameters on Agrobacterium-mediated transformation of Chinese soybean varieties[J].Plant Cell Reports，2008，27:489-498.

[4]李文霞，李文滨，吕文河，等.农杆菌介导大豆子叶节系统的两个问题突破[J].大豆科学，2008，27(1):173-175.(Li W X,Li W B,LüW H,et al.Breakthrough of two questions on the?Agrobacterium-mediated soybean cotyledonary node systems[J].Soybean Science,2008，27(1):173-175.)

[5]武小霞，李静，刘伟婷，等.大豆农杆菌子叶节转化菌株适宜生长时期及浸染浓度的研究[J].东北农业大学学报，2010，41(1):1-6.(Wu X X,Li J,Liu W T,et al.Optimization study on strain cultivation period and infectious concentration on soybean cotyledonary node via Agrobacterium-mediated transformation system[J].Journal of Northeast Agricultural University,2010，41(1):1-6.)

[6]段莹莹,赵琳,陈李淼,等.农杆菌介导的大豆子叶节和下胚轴转化方法的比较及优化[J].大豆科学，2010,29(4):590-593.（Duan Y Y,Zhao L,Chen L M,et al.Comparison and optimization of the Agrobacterium-mediated transformation of soybean by using cotyledonary node and hypocotyl explants[J].Soybean Science,2010,29(4):590-593.）

[7]赵晓雯，吴芳芳，狄少康，等.农杆菌介导的大豆子叶节遗传转化技术流程及操作要点[J].大豆科学，2011，30(3):363-368.(Zhao X W,Wu F F,Di S K,et al.Technique flow and key operation points of Aagrobacterium-mediated genetic transformation of soybean cotyledonaryi-node[J].Soybean Science,2011,30(3):363-368.)

[8]王凤敏，李涛，王云杰，等.影响农杆菌介导大豆子叶节遗传转化因素的研究[J].大豆科学，2011，30(4)：557-562.(Wang F M,Li T,Wang Y J,et al.Assessment of factors affecting soybean cotyledonary-node Agrobacterium-mediated genetic transformation[J].Soybean Science,2011,30(4):557-562.)

[9]Zhang Z Y,Xing A Q,Staswick P,et al.The use of glufosinate as a selective agent in Agrobacterium-mediated transformation of soybean[J].Plant Cell,Tissue and Organ Culture,1999,56:37-46.

[10]唐晓飞，刘丽君，张小明，等.高产大豆新品系哈交5337和哈交5489再生条件的优化[J].大豆科学，2008，27(2):203-207.(Tang X F,Liu L J,Zhang X M,et al.Improvement of regeneration system in high-yield soybean lines Hajiao 5337 and Hajiao 5489[J].Soybean Science, 2008，27(2):203-207.)

[11]卜云萍，李明春，胡国武，等.大豆子叶节组培再生系统与农杆菌介导的基因转化系统的比较研究[J].南开大学学报(自然科学版)，2003，36(1):103-108.(Bu Y P,Li M C,Hu G W,et al.The study of comparing the transformation system of Agrobacterium-mediated and regeneration system of cotyledon nod of soybean culture[J].Journal of Nankai University（Natural Sctence Editior),2003,36（1）:103-108.)

[12]姬月梅，陈受宜，李英慧，等.农杆菌介导大豆子叶节遗传转化体系的优化研究[J].大豆科学，2008,27(1):26-32.(Ji Y M,Chen S Y,Li Y H,et al.Optimization of genetic transformation system from soybean cotyledon mediated by Agrobacterium[J].Soybean Science,2008,27(1):26-32.)

[13]李文霞，宁海龙，吕文河，等.农杆菌介导大豆子叶节转化系统的优化[J].中国农业科学，2008，41(4):971-977.(Li W X,Ning H L,LüW H,et al.Optimization of theAgrobacterium-mediated transformation systems of soybean cotyledonary node[J].Scientia Agricultura Sinica,2008，41(4):971-977.)

[14]范红军，李敏，周延清，等.农杆菌介导的大豆反义f ad2-1基因转化烟草研究[J].河南师范大学学报(自然科学版)，2010,38(2):152-155.(Fan H J,Li M,Zhou Y Q,et al.Transfer of antisense fatty acid desaturase gene from soybean to tobacco plantlets(Nicotiana tabaccu)via Agrobacterium-mediated transformation[J].Journal of Henan Normal University(Natural Science),2010,38(2):152-155.)

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(02):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(02):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(02):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(02):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(02):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(02):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(02):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(02):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(02):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(02):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

基金项目：转基因生物新品种培育重大专项资助项目（2008ZX08004003-005）。

第一作者简介：杨喆（1978-），男，在读博士，研究方向为大豆遗传育种与植物生物技术。E-mail:yz78100@163.com。
通讯作者：刘丽君（1958-），女，研究员，从事大豆遗传育种工作。E-mail:nkyssbd@126.com。

更新日期/Last Update: 2014-08-15