YAN Fan,SUN Xin,ZHAI Ying,et al.Optimization on the Regeneration System of Soybean Embryonic Tips[J].Soybean Science,2011,30(05):757-759,763.[doi:10.11861/j.issn.1000-9841.2011.05.0757]
大豆胚尖再生体系的研究
- Title:
- Optimization on the Regeneration System of Soybean Embryonic Tips
- 文章编号:
- 1000-9841(2011)05-0757-03
- Keywords:
- Soybean; Embryonic tip; genotype; Browning rate; Rooting
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以吉大豆1号、2号等5个大豆品种为材料,胚尖为外植体,研究不同浓度6-BA对不同基因型胚尖丛生芽诱芽率和再生率的影响,筛选得到适合于胚尖再生系统的基因型吉大豆2号,并以吉大豆2号为材料,研究侵染阶段加入脯氨酸和硝酸银对胚尖褐化率的影响,将获得的再生植株进行生根试验。结果表明:共培养基中添加3.0 mg·L-1脯氨酸和3.0 mg·L-1硝酸银能显著抑制褐化率,蔗糖配合较高浓度的IBA能更好地促进生根。
- Abstract:
- In order to screen the optimal genotypes for embryonic tip regeneration system in soybean(Glycine max L.Merr.),the embryonic tips of five soybean cultivars including Jidadou 1 and Jidadou 2,were used as explants to study the effects of 6-benzyl aminopurine(6-BA)concentration on induction and regeneration rate of adventitious bud.The screened soybean cultivar Jidadou 2,fitting for soybean embryonic tip regeneration system,were used as explants to study the influence of Indole-3-butytric acid(IBA)on browning rate of embryonic tips when adding proline and silver nitrate.The browning rate was effectivly inhibited when the concentration of 3.0 mg·L-1 proline and 3.0 mg·L-1silver nitrate were as the additives in co-cultivation medium.Sucrose with a high concentration of IBA could increase the rooting ratio.
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备注/Memo
基金项目:转基因生物新品种培育重大专项子课题(2008ZX08004-003);国家自然科学基金面上项目(30971808);吉林省科技发展计划重点项目(20080204);长春市科技局国际科技合作项目(08GH10);“211工程”三期重点学科建设资助项目。