YU Li-li,TAN Xiao-li,HOU Wen-sheng.Enzyme Activity Analysis of GmLACS in Saccharomyces Cerevisiae[J].Soybean Science,2011,30(05):719-722.[doi:10.11861/j.issn.1000-9841.2011.05.0719]
大豆长链脂酰辅酶A合成酶基因GmLACS在酵母中的表达
- Title:
- Enzyme Activity Analysis of GmLACS in Saccharomyces Cerevisiae
- 文章编号:
- 1000-9841（2011）05-0719-04
- 关键词:
- 大豆; 长链脂酰辅酶A合成酶; 进化树分析; 酵母互补
- Keywords:
- Soybean; LACS; Phylogenic analysis; Yeast complementation
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 在LACS缺陷型酵母YB525中对已经克隆的大豆长链脂酰辅酶A合成酶基因（GmLACS）进行表达，并对其功能进行了预测。进化树比对分析显示大豆长链脂酰辅酶A合成酶（GmLACS）与其它植物的LACSs具有高度的同源性；酵母互补测试显示GmLACS蛋白能够互补LACS缺陷型酵母YB525，具有长链脂酰辅酶A合成酶活性；底物偏好性分析显示GmLACS偏好活化大豆中的重要的长链脂肪酸。以上研究结果表明：大豆长链脂酰辅酶A合成酶基因GmLACS编码的蛋白属于LACS家族,能够活化外源脂肪酸并参与了脂肪酸相关的脂类代谢。
- Abstract:
- In this paper,GmLACS was transformed into LACS-deficient Saccharomyces cerevisiae YB525 to analyze its enzymatic activity.Phylogenic analysis indicated GmLACS showed a high degree of homology with other LACSs of plants.GmLACS could complement the YB525 mutant which was deficient in LACS showing that GmLACS belonged to the LACS family.The transformants preferred to use long chain fatty acids indicated GmLACS could activate the free fatty acids and involve the metabolism of fatty acids and lipids.
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备注/Memo
基金项目：国家科技重大专项资助项目（2008ZX08010-004）。