LI Xiao-ming,DU Chun-mei,ZHENG Nan,et al.Inhibitory Effect of Bacillus BL-21,HNDF2 on Soybean Cyst Nematode[J].Soybean Science,2011,30(04):710-712.[doi:10.11861/j.issn.1000-9841.2011.04.0710]
芽孢杆菌BL-21、HNDF2对大豆胞囊线虫抑制效果的研究
- Title:
- Inhibitory Effect of Bacillus BL-21,HNDF2 on Soybean Cyst Nematode
- 文章编号:
- 1000-9841（2011）04-0710-03
- Keywords:
- Bacillus; Soybean cyst nematode; Inhibition
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 从黑龙江大豆胞囊线虫病已发生衰退的地块中分离得到的2株生防菌株BL-21和HNDF2，试验考察了BL-21和HNDF2菌液及菌株发酵液处理对大豆胞囊线虫胞囊孵化和二龄幼虫产生的影响。结果表明：菌液处理的效果好于除菌发酵液处理， BL-21与HNDF2的混合菌液抑制作用最明显，对胞囊孵化的相对抑制率达到80.93%，对二龄幼虫的校正死亡率达到91.08%，孵化出的线虫数和二龄幼虫死亡率均与对照呈极显著差异。
- Abstract:
- Two Bacillus BL-21and HNDF2 were isolated from recessive land of soybean cyst nematode in Heilongjiang province,the effect of their bacteria liquid and fermentation filtrate on cyst hatching and J2 were observed.There were 6 treatments including the bacteria liquids and fermentation filtrates of BL-21, HNDF2 and their mixture, respectively. The effect of bacteria liquid was better than fermentation filtrate.The mixed bacteria liquid of two Bacillus strains had best inhibition effect on cyst hatching and lethality of J2, its relative inhibition rate of cyst hatching were 80.93%,the corrected mortality rate of J2 reached 91.08%,which were significantly different with water control.Results suggest that the mixed bacteria liquid of BL-21 and HNDF2 had the best inhibition effect on soybean cyst nematode.
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备注/Memo
基金项目：国家转基因作物新品种培育重大专项资助项目 (2008ZX08004-002)。