FANG Biao,WANG Pi-wu,YU Lei,et al.Cloning and Transformation of Soybean Lectin Gene Le2 into Tobacco[J].Soybean Science,2011,30(04):706-709.[doi:10.11861/j.issn.1000-9841.2011.04.0706]
大豆凝集素基因Le2克隆及转化
- Title:
- Cloning and Transformation of Soybean Lectin Gene Le2 into Tobacco
- 文章编号:
- 1000-9841（2011）04-0706-04
- Keywords:
- Soybean lectin; Genetic engineering; Insect resistance; Agrobacterium; Tobacco
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 大豆凝集素对提高植物的抗虫性有重要作用。应用基因工程手段，克隆大豆凝集素基因Le2，构建植株表达载体pSy-Le2，将大豆凝集素Le2基因通过农杆菌介导法导入烟草中，经过筛选获得转基因植株，为进一步鉴定Le2的基因功能提供了试验基础。
- Abstract:
- Soybean lectin(Soybean agglutinin) is a major protein in soybean，which plays an important role in plant insects resistance as a main anti-nutritional factors.In this study,the soybean lectin gene Le2 had been cloned by genetic engineering technology,then constructed the recombinant plant expression vector pSy-Le2 and transformed it into tobacco by Agrobacterium mediated way.Transgenic plant had been selected,which supplied basic materials for further experiments.
参考文献/References:
[1]高燕会，李润植，毛雪,等.植物凝集素的防卫功能及其研究进展[J].世界农业，2000(2)：33-35.(Gao Y H,Li R Z,Mao X,et al.Plant lectin defence function and its research progress[J].World Agriculture,2000(2):33-35.) [2]潘洪彬，秦贵信，孙泽威.大豆凝集素抗营养的研究进展[J].大豆科学，2005，24(3)：210-215.(Pan H B,Qin G X,Sun Z W.Soybean lectin antioxidant nutrients research progress[J].Soybean Science,2005,24(3):210-215.) [3]李凤玲，徐培洲，肖祎,等.植物凝集素及其在抗虫基因工程中的应用[J].安徽农业科学，2006，34(3)：430-432，439.(Li F L,Xu P Z,Xiao W.Plant lectin and insect-resistant genes engineering application[J].Journal of Anhui Agricultural Sciences,2006,34(3):430-432,439.) [4]张柏林，秦贵信，刘宁,等.大豆凝集素结构及其活性测定方法的研究进展[J].大豆科学，2009，28(1)：160-163.(Zhang B L,Qin G X,Liu N.Advances of research on structure and activity detecting method of soybean agglutinin[J].Soybean Science,2009,28(1):160-163.) [5]Lotan R,Siegelman H W,Lis H,et al.Subunit structure of soybean agglutinin [J].Journal of Biological Chemisty,1974,249:1219-1224. [6]王关林,方宏筠.植物基因工程[M]//方宏筠.抗植物虫害基因及其应用.北京：科学出版社，2005：532.(Wang G L,Fang H J.Plant gene engineering[M]// Fang H J.Resistance to plant pest gene and its application.Beijing:Science Press,2005：532.) [7]Hofen R,Willmitzer L.Storage of competent cells for Agrobacterium?transformation[J].Nucleic Acids Research,1988,16:9877. [8]刘辰，谢柳，张文飞.新型Bt杀虫蛋白：VIP杀虫的机理与植物转基因应用[J].分子植物育种，2008，6(6)：1031-1037.(Liu C,Xie L,Zhang W F.Insecticidal mechanism of Bt vegetative insecticidal proteins and their application in GM crops[J].Molecular Plant Breeding,2008,6(6):1031-1037.) [9]武小霞，李文滨.大豆抗虫基因工程研究进展及发展趋势[J].东北农业大学学报，2005，36(4)：502-506.(Wu X X,Li W B.Research progress and development direction of soybean pest-resistance gene engineering[J].Journal of Northeast Agricultural University,2005,36(4):502-506.) [10]郭红.抗病、抗虫转基因植物的研究与应用[J].现代农业科技，2010（10）：85-87.(Guo H.Disease resistance,resistance to insects the research and application of transgenic plants[J].Modern Agricultural Sciences and Technology,2010（10）:85-87.)
相似文献/References:
[1]魏 强,李鲁华,王春艳,等.大豆凝集素le2基因RNA干扰表达载体构建及转化的研究[J].大豆科学,2013,32(03):306.[doi:10.11861/j.issn.1000-9841.2013.03.0306]
WEI Qiang,LI Lu-hua,WANG Chun-yan,et al.Construction of RNAi Expressed Vector of Soybean Agglutinin le2 Gene and Transform Research[J].Soybean Science,2013,32(04):306.[doi:10.11861/j.issn.1000-9841.2013.03.0306]
[2]杨 巧,刘 倩,简 恒.RNAi技术在抗大豆胞囊线虫基因工程研究中的应用[J].大豆科学,2013,32(04):548.[doi:10.11861/j.issn.1000-9841.2013.04.0548]
YANG Qiao,LIU Qian,JIAN Heng.Technology of RNAi and Its Application in Genetic Engineering against Soybean Cyst Nematode of Soybean[J].Soybean Science,2013,32(04):548.[doi:10.11861/j.issn.1000-9841.2013.04.0548]
[3]韩阳,曹永强,赖冰冰.植物抗病基因工程育种策略及其在大豆上的应用[J].大豆科学,2009,28(06):1103.[doi:10.11861/j.issn.1000-9841.2009.06.1103]
HAN Yang,CAO Yong-qiang,LAI Bing-bing.Strategies of Genetic Engineering Breeding for Plant Disease Resistance and Its Application in Soybean[J].Soybean Science,2009,28(04):1103.[doi:10.11861/j.issn.1000-9841.2009.06.1103]
[4]张柏林,秦贵信,孙泽威,等.大豆凝集素处理对肉仔鸡生长性能及小肠发育的影响[J].大豆科学,2010,29(02):292.[doi:10.11861/j.issn.1000-9841.2010.02.0292]
ZHANG Bo-lin,QIN Gui-xin,SUN Ze-wei,et al.Effects of Soybean Agglutinin on Broiler Growth Performance and Small Intestine Development[J].Soybean Science,2010,29(04):292.[doi:10.11861/j.issn.1000-9841.2010.02.0292]
[5]张柏林,秦贵信,刘宁,等.大豆凝集素结构及其活性测定方法的研究进展[J].大豆科学,2009,28(01):160.[doi:10.11861/j.issn.1000-9841.2009.01.0160]
ZHANG Bo-lin,QIN Gui-xin,LIU Ning,et al.Advances of Research on Structure and Activity Detecting Method of Soybean Agglutinin[J].Soybean Science,2009,28(04):160.[doi:10.11861/j.issn.1000-9841.2009.01.0160]
[6]董志敏,王曙明,刘玉芝,等.大豆抗菌核病研究进展[J].大豆科学,2008,27(06):1053.[doi:10.11861/j.issn.1000-9841.2008.06.1053]
DONG Zhi-min,WANG Shu-ming,LIU Yu-zhi,et al.Progress on Resistance to Sclerotinia Sclerotiorum in Soybean[J].Soybean Science,2008,27(04):1053.[doi:10.11861/j.issn.1000-9841.2008.06.1053]
[7]王涛,秦贵信,赵元,等.大豆主要抗营养因子对猪的影响[J].大豆科学,2008,27(02):326.[doi:10.11861/j.issn.1000-9841.2008.02.0326]
WANG Tao,QIN Gui-xin,ZHAO Yuan,et al.Effects of Main Soybean Antinutritional Factors on Swine[J].Soybean Science,2008,27(04):326.[doi:10.11861/j.issn.1000-9841.2008.02.0326]
[8]王利民,秦贵信,刘林娜,等.三种亲和层析体系纯化大豆凝集素的比较研究[J].大豆科学,2007,26(01):51.[doi:10.3969/j.issn.1000-9841.2007.01.012]
WANG Li-min,QIN Gui-xin,LIU Lin-na,et al.COMPARISON STUDY OF THREE AFFINITY CHROMATOGRAPHY SYSTEMS ON SOYBEAN AGGLUTININ PURIFICATION[J].Soybean Science,2007,26(04):51.[doi:10.3969/j.issn.1000-9841.2007.01.012]
[9]吴莉芳,秦贵信,孙玲,等.大豆凝集素及其对动物健康的影响[J].大豆科学,2007,26(02):259.[doi:10.3969/j.issn.1000-9841.2007.02.029]
WU Li-fang,QIN Gui-xin,SUN Ling,et al.SOYBEAN AGGLUTININ AND THE EFFECTS ON ANIMAL HEALTH[J].Soybean Science,2007,26(04):259.[doi:10.3969/j.issn.1000-9841.2007.02.029]
[10]梁慧珍,李卫东,许阳,等.大豆雄性不育遗传及基因工程创造途径[J].大豆科学,2004,23(04):296.[doi:10.11861/j.issn.1000-9841.2004.04.0296]
[J].Soybean Science,2004,23(04):296.[doi:10.11861/j.issn.1000-9841.2004.04.0296]
备注/Memo
基金项目： 国家转基因专项资助项目（2011ZX08004-004）；大学生科技创新基金资助项目。