LI Bo,FU Yong-ping,WANG Pi-wu,et al.Construction of Plant Expression Vector Involving HrpZpsta Gene and IIts Transformation into Soybean[J].Soybean Science,2011,30(03):393-396.[doi:10.11861/j.issn.1000-9841.2011.03.0393]
HrpZpsta基因植物表达载体的构建及其在大豆中的转化
- Title:
- Construction of Plant Expression Vector Involving HrpZpsta Gene and IIts Transformation into Soybean
- 文章编号:
- 1000-9841(2011)03-0393-04
- 关键词:
- 大豆; hrpZpsta基因; 表达载体构建; 转基因植株
- Keywords:
- Soybean; hrpZpsta gene; Vector construction; Transgenic plants
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 利用PCR技术扩增含有hrpZpsta基因的克隆载体pMD18-T-hrpZpsta，以植物表达载体pBI121为基础，将PCR产物插入到此植物表达载体中。利用农杆菌介导的大豆子叶节转化法将构建好的表达载体导入大豆品种吉农28中,选用筛选条件为100 mg·L－1的卡那霉素选择培养基培养，对获得的转基因植株进行PCR检测，结果从部分抗性植株中扩增出hrpZpsta基因，初步证明hrpZpsta基因成功转入到受体大豆中，获得了转基因阳性植株。
- Abstract:
- The hrpZpsta gene has broad disease-resistance and can improve the antiviral ability of plants. In this study, the hrpZpsta gene had been cloned from the cloning vector pMD18-T-hrpZpsta, and inserted into pBI121 to construct the vector pBI121-hrpZpsta. pBI121-hrpZpsta was transformed into cotyledon nodes of soybean Jinong 28 by ?Agrobacterium-mediated method. Kanamycin (100 mg·L－1) was applied to select the transformed tissue in selecting medium. Extracted DNA among the transgenic plants and analyzed it by PCR. The positive results indicated that hrpZpsta gene was successfully transformed into the genome of transgenic soybean.
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备注/Memo
基金项目：国家转基因专项资助项目（2011ZX08004-004）。