YANG Zhe,WANG Fang-mei,WANG Han,et al.Hydrogen Peroxide Involved in Regulation Aluminum Toxicity in Root Tips of Soybean[J].Soybean Science,2011,30(01):105-108.[doi:10.11861/j.issn.1000-9841.2011.01.0105]
参与大豆根尖铝毒调节的研究
- Title:
- Hydrogen Peroxide Involved in Regulation Aluminum Toxicity in Root Tips of Soybean
- 文章编号:
- 1000-9841（2011）01-0105-04
- Keywords:
- Soybean; Aluminum toxicity; H2O2; Alleviating effect
- 分类号:
- X503.231
- 文献标志码:
- A
- 摘要:
- 以浙春3号大豆为材料，研究100 μmol·L-1 Al3+处理下，外源H2O2对大豆铝毒的缓解效应以及可能的生理生化调节机理。结果表明：0.1 mmol·L-1 H2O2显著降低根尖Al含量，减轻了根尖活性氧(ROS)的积累及质膜通透性，增强了SOD和CAT活性，并且该缓解作用可以被150 U·mL-1 过氧化氢酶(CAT)消除。表明H2O2作为信号分子可以通过诱导保护酶活性的提高来缓解铝毒害。
- Abstract:
- The alleviating effect of exogenous H2O2 on aluminum (Al) toxicity and its possible mechanisms of physiological and biochemical regulation were studied in soybean. Pretreatment with 0.1 mmol·L-1 H2O2 during soybean exposure to Al significantly reduced aluminum content, active oxygen species accumulation and membrane permeability in the root tip. Pretreatment with H2O2 also increased the protective enzyme activity of SOD and CAT. Moreover, the alleviating effect was reversed by the addition of H2O2 scavenger CAT. These findings indicated that H2O2 act as a signal molecule involved in the protection of plants against Al toxicity by enhancing protective enzyme activity.
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备注/Memo
基金项目：国家自然科学基金资助项目(30800705)；浙江省自然科学基金资助项目(Y307535, Y304185)。