LUAN Bai,GAO Tong-guo,JIANG Feng,et al.Effect of Fulvic Acid (FA) from Lignite Degraded on Germination Rate and Activities of Main Antioxidases in Soybean Seedlings[J].Soybean Science,2010,29(04):607-610.[doi:10.11861/j.issn.1000-9841.2010.04.0607]
微生物降解褐煤产生的黄腐酸对大豆种子萌发及主要抗氧化酶活性的影响
- Title:
- Effect of Fulvic Acid (FA) from Lignite Degraded on Germination Rate and Activities of Main Antioxidases in Soybean Seedlings
- 文章编号:
- 1000-9841（2010）04-0607-04
- Keywords:
- Soybean; Fulvic acid; Biodegradation; Antioxidase
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 研究了微生物降解褐煤产生的黄腐酸（FA）对大豆萌发过程中过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)的影响，以期揭示FA促进种子萌发的机理。结果表明：适宜浓度FA可以提高大豆萌发率，显著增加种子POD、CAT及SOD的活性。FA浓度为100 mg·kg-1时，CAT、SOD活性分别提高32.15%、24.5%；FA浓度为200 mg·kg-1时，大豆萌发率比对照提高34.6%，并且POD活性增加19.92%。而高浓度的黄腐酸对大豆萌发及抗氧化酶活性产生抑制作用。FA表现出类似生长调节剂的性质，适宜浓度的黄腐酸能提高大豆种子的萌发率，显著提高其抗氧化酶的活性，对种子萌发起到促进作用。
- Abstract:
- The antioxidase system can remove reactive oxygen and play an important role in the process of soybean germination. To study how fulvic acid (FA),produced by microbial degraded lignite, can improve the germination, Spectrophotometry and nitroblue tetrazolium test (NBT) ilumination method were taken to detect the activities of peroxidase (POD) , catalase (CAT) and superoxide dismutase (SOD). The results showed when the concentration of FA was 100 mg·kg-1, the activities of CAT and SOD were improved by 32.15% and 24.5%,respectively. The germination ratio increased by 34.6% under a condition where the concentration of FA was 200 mg·kg-1,and the activity of POD was also increased by 19.92% under this FA concentration. In contrast with the promotion effect, solution with high FA concentration can restrain the activities of antioxidase. The study has shown that appropriate concentration of FA from lignite degraded by microorganism can improve the germination of soybean and increase the activities of POD, CAT and SOD significantly. FA is something like plant growth regulator, the appropriate concentration of FA can improve the germination ratio of soybean seed and improve its activities of antioxidase apparently.
参考文献/References:
[1]张立军，宋广州，白霜，等. H2O2对不同大豆品种低温萌发及主要抗氧化酶活性的影响[J]. 大豆科学，2008，27(1):97-105.(Zhang L J, Song G Z, Bai S, et al. Effect of exogenous H2O2 on germination rate and activities of main antioxidase in soybean (Glycine maxL.) seedlings under low temperature[J]. Soybean Science, 2008,27(1):97-105.) [2]郝晶，张立军，谢甫绨. 低温对大豆不同耐冷性中萌发期保护酶活性的影响[J]. 大豆科学, 2007,26(2):171-175.( Hao J, Zhang L J, Xie F T. Effects of the low temperature on defense enzyme activities of different chilling-tolerant soybean cultivars during the germination [J]. Soybean Science, 2007,26(2):171-175.) [3]Adani F, Genevini P, Zaccheo P, et al. The effect of commercial humic acid on tomato plant growth and mineral nutrition[J]. Journal Plant Nutrure,1998,21(3):561-575. [4]程渡，彭玉梅，崔鲜一，等.黄腐酸旱地龙对农作物、牧草应用效果试验[J].腐植酸,1996(3):33-34.(Cheng D, Peng Y M, Cui X Y, et al. The effect test of applying handilong and fulvic acid to crops and Herd’s grass[J]. Humic Acid, 1996(3):33-34.) [5]袁红莉,姜峰,高同国.微生物降解褐煤产生黄腐酸的研究[J]. 腐植酸,2009(5):1-4.( Yuan H L, Jiang F, Gao T G. Study on biotechnology application in producing fulvic acid by degraded lignite [J]. Humic Acid, 2009(5):1-4.) [6]袁红莉，王凤芹，李宝珍，等.微生物降解褐煤产生的腐殖酸生物活性研究[J].土壤学报，2002,39（增刊）:129-134. (Yuan H L, Wang F Q, Li B Z, et al. The biological active research of humic acid from lignite degraded [J]. Acta Pedologica Sinica, 2002,39（Suppl）:129-134.) [7]袁红莉，周希贵，陈文新，等.微生物降解褐煤产生的腐植酸化学特性研究[J].环境化学，2000，19(3):240-243.( Yuan H L, Cai Y Q, Zhou X G , et al.Study on chemical properties of humic acid of microboal degraded lignite[J]. Environmental Chemistry, 2000, 19(3):240-243.) [8]朱京涛，曹霞,王学东.生物黄腐酸对番茄幼苗发育及产量品质的影响[J]. 安徽农业科学,2009, 37(3)：964-965，968.(Zhu J T，Cao X，Wang X D. Study on effects of the biological humic acid on seedlings growth and quality of tomato [J]. Journal of Anhui Agricultural Sciences,2009,37(3):964-965,968.) [9]苏桂华，王云忠，藩世华.黄腐酸药浴治疗良性关节肿痛328例[J].腐植酸，1996 (2):35-45. (Su G H, Wang Y Z, Pan S H. The 328th case of fulvic acid dipping treatment to cure the swelling and pain of benign joint[J].Humic Acid, 1996(2):35-45.) [10]李绪行，邵莉楣，殷蔚薏，等.黄腐酸和汪平酸对小麦种子萌发与幼苗生长及绿豆下胚轴生根的影响[J].植物学通报，1991，8(3):51-55.( Li X H, Shao L M, Yin W Y,et al. The influencences of fulvic acid and wangping acid on seed germination and seedling growth of wheat and rhizogenic action of mung bean [J]. Chinese Bulletin of Botany，1991，8（3）：51-55.) [11]李绪行，殷蔚薏，邵莉楣，等.黄腐酸增强小麦抗旱能力的生理生化机制初探[J].植物学通报，1992，9(2):44-46.(Li X H,Yin W Y,Shao L M,et al. Preliminary investigation of physiological and biochemical mechanisms on drought-resistance of wheat enhanced by leaf-spraying fulvic acid [J]. Chinese Bulletin of Botany,1992,9(2):44-46.) [12]王高伟，胡光洲，孔倩，等.煤炭腐植酸的基本性能及其工农业应用[J].煤炭技术，2007，26(11):111-114.( Wang G W，Hu G Z，Kong Q, et al .Properties of humic acid in coal and its applications in industry and agriculture[J]. Coal Technology, 2007, 26(11):111-114.) [13]袁红莉，陈文新. 微生物降解生产黄腐酸方法：中国,02158189.4[P].2004-07-07. (Yuan H L, Chen W X. Methods of manufacturing fulvic acid by micro-biological degradation：PR China，02158189.4[P].2004-07-07.) [14]张德和．风化煤黄腐酸的结构表征[J]．化学学报，1981，39(5):401-408. （Zhang D H. Structure characterizations of weathered coal fulvic acid[J]. Chemistry Academic Journal, 1981,39(5):401-408.） [15]张志良.植物生理学实验指导[M]. 北京：高等教育出版社, 1990:154-156.(Zhang Z L. Guidance of plant physiology experiment [M]. Beijing: Higher Education Press, 1990:154-156.) [16]Kraus T E, Fletcher R A. Wheat seedlings from heat and paraquat injury is detoxification of active oxygen involved[J]. Plant Cell Physiology, 1994, 35(1):45-52. [17]朱广廉，钟海文，张爱琴，等. 植物生理学实验指导[M]. 北京：北京大学出版社,1990：242-245.（Zhu G L, Zhong H W, Zhang A Q,et al. Guidance of plant physiology experiment [M]. Beijing:Peking University Press,1990:242-245.） [18]程扶玖,杨道麒,吴庆生. 黄腐酸对油菜种子萌发、氮素吸收及抗寒性的生理效应[J]. 安徽农业大学学报，1995（2）:123-128.(Cheng F J, Yang D Q, Wu Q S. Physiological effects of fulvic acid (FA) on seed germination, nitrogen uptake and cold resistance of rape [J]. Journal of Anhui Agricultural University, 1995（2）:123-128.) [19]陈玉玲,曹敏,李云萌.干旱条件下黄腐酸对冬小麦幼苗中内源ABA和IAA以及SOD和POD活性的影响[J].植物生理学通讯, 2000,36(4):311-314.( Chen Y L, Cao M, Li Y Y. Effect of fulvic acid on aba, iaa and activities of superoxide dismu-tase and peroxydase in winter wheat seedling under drought condition [J]. Plant Physiology Communications, 2000, 36(4):311-314.) [20]Muscolo A, Felici M, Concheri G,et al. Effect of earthworm humic substances on esterase and peroxidase activity during growth of leaf explants of Nicotiana plumbaginifolia[J]. Biology and Fertility of Soils, 1993,15：127-131. [21]高同国，姜峰，李召虎，等.微生物降解褐煤产生的黄腐酸对玉米幼苗生长的影响[J].腐植酸,2009（1）:14-18.( Gao T G,Yang J S,Yuan H L.Effects of fulvic acid from lignite degraded by microbial on growth of maize seedling[J]. Humic Acid, 2009（1）:14-18.)
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备注/Memo
基金项目：国家高技术研究发展计划资助项目（2006AA10A213）；国家基础科学人才培养基金资助项目（J0730639）；科技部成果转化资助项目（2008360075）。