[1]武小霞,刘伟婷,刘琦,等.利用花粉管通道法将抗虫基因（cry V）转入大豆的研究[J].大豆科学,2010,29(04):565-568,574.[doi:10.11861/j.issn.1000-9841.2010.04.0565]
　WU Xiao-xia,LIU Wei-ting,LIU Qi,et al.Introduction of Anti-pest Gene (cry V) to Soybean via Pollen-Tube Pathway[J].Soybean Science,2010,29(04):565-568,574.[doi:10.11861/j.issn.1000-9841.2010.04.0565]
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利用花粉管通道法将抗虫基因（cry V）转入大豆的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第29卷 期数: 2010年04期 页码: 565-568,574 栏目: 出版日期: 2010-08-25
Title:
Introduction of Anti-pest Gene (cry V) to Soybean via Pollen-Tube Pathway
文章编号:
1000-9841（2010）04-0565-04
作者:
武小霞1; 刘伟婷2; 刘琦3; 李静1; 马永4; 李文滨1
1．东北农业大学 农学院，大豆生物学省部共建教育部重点实验室，黑龙江 哈尔滨150030；
2．黑龙江省农业科学院 植物脱毒苗木研究所,黑龙江 哈尔滨 150086；
3.黑龙江省农业科学院 园艺分院，黑龙江 哈尔滨 150086；
4.七台河市新兴区长兴乡人民政府农业综合服务中心，黑龙江 七台河 154532
Author(s):
WU Xiao-xia1;  LIU Wei-ting2;  LIU Qi3;  LI Jing1;  MA Yong4;  LI Wen-bin1
1.Agricultural College of Northeast Agricultural University,Chinese Education Ministry’s and Provincial Key Laboratory of Soybean Biology, Harbin 150030；
2.Plant Virus-free Nursery Stock Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086;
3.Horticulture Branch of Heilongjiang Academy of Agricultural Sciences, Harbin 150086;
关键词:
大豆; 抗虫基因（cry V）; 花粉管通道法
Keywords:
Soybean; Anti-pest gene（cry V）; Pollen-tube pathway
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.04.0565
文献标志码:
A
摘要:
利用花粉管通道法，在大豆的盛花期将外源抗虫基因（cry V）转入到大豆中，转化成活率达43.88%，成荚率达33.6%。将收获的的种子种植在大豆所温室中，通过卡那霉素筛选、PCR鉴定，Southern-blot检测和RT-PCR检测，共检测出5株阳性植株，确定外源抗虫基因（cry V）已转入到大豆中，转化率达1.8‰。
Abstract:
Anti-pest gene (cry V) was introduced to soybean via pollen-tube pathway during blossom period. 1211 flowers out of 2760 ones survived 3 days after the operation, the survival rate and podding rate was 43.88%and 33.6%, respectively. The harvested seeds were planted in greenhouse and 5 plants with positive result were obtained after Km screening, PCR identification, southern-blot testing and RT-PCR testing. Results suggestted that the anti-pest gene (cry V) were successfully introduced into those 5 plants and the transformation rate was 1.8‰.

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备注/Memo

基金项目：转基因生物新品种培育重大专项资助项目（2008ZX08004-002，2009ZX08009-089B）；哈尔滨市科技创新人才研究专项资金资助项目（2008RFQXN013）；东北农业大学博士后基金与博士启动基金资助项目（2009RC47）。

第一作者简介：武小霞（1971-），女，博士，副研究员，研究方向为大豆遗传育种及生物技术应用。E-mail: dadousuo@yahoo.com.cn。

更新日期/Last Update: 2014-09-14