ZHANG Da-wei,DU Xiang-yu,LIU Chun-yan,et al.Effect of Low-temperature Stress on Physiological Indexes of Soybean at Germination Stage[J].Soybean Science,2010,29(02):228-232.[doi:10.11861/j.issn.1000-9841.2010.02.0228]
低温胁迫对大豆萌发期生理指标的影响
- Title:
- Effect of Low-temperature Stress on Physiological Indexes of Soybean at Germination Stage
- 文章编号:
- 1000-9841(2010)02-0228-05
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 在4℃和20℃条件下,对12个大豆品种萌发期的相对电导率、丙二醛、脯氨酸和可溶性糖等生理指标进行测定,并对其萌发期耐低温性进行综合性评价。结果表明:随着4℃处理时间的延长,电导率、丙二醛、脯氨酸和可溶性糖的含量都升高;4℃与20℃电导率的差值略有升高,其它都降低;只有相对电导率差值的均值变化趋势与4℃变化动态一致。按照上述指标将供试品种萌发期的耐低温性分为3类:绥农14、垦丰7号、黑农48、合丰25和垦农18的耐低温性强,红丰11、铁6915和辽豆21耐低温性次之,垦鉴豆35、海南2号、合丰50和NOVA耐低温性差。结果为进一步完善防御大豆低温冷害措施和进行耐低温性生理生化育种提供理论依据。
- Abstract:
- Soybean is sensitive to low temperature, especially in initial period of germination. Relative conductivity, malondialchehyche(MDA), proline, and soluble sugar of twelve soybean varieties at stage of germination were measured in 4℃ and 20℃. The comprehensive evaluation and cluster analysis were conducted for them. The results show that relative conductivity, MDA, proline, and soluble sugar increased with the time at 4℃, difference value between 4℃ and 20℃in soluble sugar is increased slightly and the others are decreased, mean trend of difference value in relative conductivity is consistent with change dynamics at 4℃. The most tolerance varieties are Suinong 14, Kenfeng 7, Heinong 48, Hefeng 25, and Kennong 18. On the contrary, Kenjiandou 35, Hainan 2, Hefeng 50, and NOVA are the least. It is provided a theoretical basis for protecting of chilling damage and breeding varieties with low temperature tolerance.
参考文献/References:
[1]宋剑陶,顾增辉.大豆抗冷性生理生化指标的筛选[J]. 中国农业科学,1992, 25(4): 15-23.(Song J T, Gu Z. Studies on screening for physiological and biochemical indicators of chilling resistance of soybean (Glycine maxL.Merr.)[J]. Scientia Agricultura Sinica, 1992, 25(4):15-23.) [2]郝晶,张立军,谢甫绨.低温对大豆不同耐冷性中萌发期保护酶活性的影响[J]. 大豆科学,2007, 26(2): 171-175. (Hao J, Zhang L J, Xie P D. Effects of the low temperature on defense enzyme activities of different chilling-tolerant soybean cultivars during the germination[J]. Soybean Science, 2007, 26(2): 171-175.) [3]单彩云.大豆耐低温资源筛选及蛋白质组学研究[D].哈尔滨:东北农业大学,2008.(Shan C Y. Screening and proteome research of soybean low temperature tolerance germplasm[D]. Harbin: Northeast Agricultural University, 2008.) [4]郝再彬,苍晶,徐仲.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社, 2004.(Hao Z R, Cang J, Xu Z. Plant physiology experiment[M].Harbin:Harbin Institute of Technology Press, 2004.) [5]时连辉.土壤水分胁迫对不同品种桑树的影响[D].济南:山东农业大学,2004.(Shi L H. Effect of soil water stress on different mulberry varieties [D].Jinan: Shandong Agricultural University, 2004.) [6]刘宁,高玉葆,贾彩霞. 渗透胁迫下多花黑麦草叶内过氧化物酶活性和脯氨酸含量以及质膜相对透性的变化[J]. 植物生理学通讯,2000, 36(1): 11-14.(Liu N, Gao Y B, Jia C X. Changes in POD activity, free proline content and cytomembrane permeability of lolium multiflorum leaves under different levels of osmotic stress[J].Plant Physiology Communications,2000, 36(1): 11-14.) [7]刘祖棋,张石城.植物抗性生理学[M].北京:中国农业出版社,1994.(Liu Z Q, Zhang S C. Physiology of plant resistance [M].Beijing: Agricultural Press,1994.) [8]Dhindsa R S. Drought stress, enzymes of glutathione metabolism, oxidation injury, and protein synthesis in tortulia turalis [J]. Plant Physiology, 1991, 95: 648-651. [9]Delauney A J, Verma D P S. Proline biosynthesis and osmoregulation in plants [J]. Plant Journal, 1993, 4(2): 215-223. [10]Ana Santa-Cruz, Manuel A costa, Ana Rus, et al. Short-term salt tolerance mechanisms in differentially salt tolerant tomato species [J]. Plant physiology,1999, 37(1): 65-71. [11]Smirnoff N, Cumber Q J. Hydroxyl radical scavenging activity of compatible solutes [J]. Phytochemistry, 1989, 28: 1057-1060. [12]Solomon A, Beer S, Waisel Y,et al. Effects of NaCl on the carboxylating activity of Rubisco from Tamarix jordanis in the presence and absence of proline related compatible solutes [J]. Plant Physiology, 1994, 90: 198-204. [13]张美云,钱吉,郑师章.渗透胁迫下野生大豆游离脯氨酸和可溶性糖的变化[J]. 复旦学报(自然科学版), 2001, 40(5): 558-561.(Zhang M Y, Qian J, Zheng S Z. Studies on free proline and soluble sugar of wild soybeans (Glycine soja) under osmotic stress [J]. Journal of Fudan University(Nature Science),2001,40(5):558-561.) [14]Cohn M A, Obendorf R L. Occurrence of a stelar lesion during imbibitional chilling of Zea maysL[J]. American Journal of Botany, 1978, 65:50-56. [15]Deswal D P, Sheoran I S. A simple method for seed leakage measurement: applicable to single seeds of any size[J]. Seed Science and Technology, 1993, 21: 179-185. [16]Lyons J M. Chilling injury in plants[J]. Plant Physiology, 1973, 20: 423-446. [17]宰学明,吴国荣,陆长梅,等.低温预处理对大豆萌芽活力及其活性氧代谢的影响[J]. 大豆科学,2001, 20(3): 163-166.(Zai X M, Wu G R, Lu C M. The effects of prechilling on vigour index and active oxygen metabolism of soybean seeds[J]. Soybean Science, 2001, 20(3): 163-166.) [18]陈少裕.膜脂过氧化与植物逆境胁迫[J].植物学通报, 1989, 6(4): 211-217.(Chen S Y. Membrane-lipid preoxidation and plant stress [J].1989,6(4):211-217.)
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备注/Memo
基金项目:国家高技术研究发展计划资助项目(2006AA100104-3);引进国际先进农业科学技术计划资助项目(2006-G1(A));国家支撑计划资助项目(2006BAD21B01);国家转基因专项资助项目(2009ZX08004-009B)。